Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 78043065

GI number: 78043065

Start: 995456

End: 996088

Strand: Direct

Name: nth [H]

Synonym: CHY_1121

Alternate gene names: 78043065

Gene position: 995456-996088 (Clockwise)

Preceding gene: 78045103

Following gene: 78044914

Centisome position: 41.45

GC content: 41.07

Gene sequence:

>633_bases
GTGACCGGAAAAAAAGCACATAAAATTGCTGCAGAGTTAGAAAAACTTTTTCCCGTGGCAAAAACGGAGTTAAATTTCCA
AAATATTTTTCAGCTGCTTGTGGCAGTGGTGTTATCGGCGCAAAGTACCGACCGGCAGGTAAATAAAGTAACGGAAAAAC
TTTTTTTATTTGTAAAGGAGCCCAGGGATTTATTAGATATGGGAGAAGAGGAGTTATCCCGGCAAATTCGTTCTTTGGGT
TTGTACCGGAATAAAGCGAGAAATTTAATTAAAATTGCTGAAATCCTGGACAGGGAGTATCATGGTCAAGTACCTGATAG
TTTTGCTGAACTTCTTAAACTTCCGGGGGTTGGACCGAAAACTGCGGAGGTTATTGTGGGAGTTGGTTTTAATAAACCTT
CCTTTCCGGTGGATACCCATGTTTTTAGGGTAGCCCGGCGTCTTGGGTTATCGAAAGCCAGAACGCCGGAAGGCGTATCT
TTTGATTTGAAAAAAATTTTTCCTCCAAATAGCTGGATAGACTTACACCATCGCTTAATTTTTTTCGGAAGAAGAATATG
TAAGGCCCAAAAGCCAAGCTGCAATATCTGTCCTTTTCCGGAATTTTGCCAAAAGGAGGAGTCCGATGTTTAA

Upstream 100 bases:

>100_bases
TATTGTTATGATTGTTTGGATTTGTTTCCTAACGGCATAAAGTTTTGTCAAACCTGCGGTGAATTTATTTGCGATGAATG
TTATGAGGGAATGGTGGAGT

Downstream 100 bases:

>100_bases
CATTGTTTTGGTGGAACCGGAAATACCTTATAATACCGGAAATATTGCTCGCACCTGTGCTGCTACCGGTTCAACCCTCC
ATCTGGTTAAACCCCTGGGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTGKKAHKIAAELEKLFPVAKTELNFQNIFQLLVAVVLSAQSTDRQVNKVTEKLFLFVKEPRDLLDMGEEELSRQIRSLG
LYRNKARNLIKIAEILDREYHGQVPDSFAELLKLPGVGPKTAEVIVGVGFNKPSFPVDTHVFRVARRLGLSKARTPEGVS
FDLKKIFPPNSWIDLHHRLIFFGRRICKAQKPSCNICPFPEFCQKEESDV

Sequences:

>Translated_210_residues
MTGKKAHKIAAELEKLFPVAKTELNFQNIFQLLVAVVLSAQSTDRQVNKVTEKLFLFVKEPRDLLDMGEEELSRQIRSLG
LYRNKARNLIKIAEILDREYHGQVPDSFAELLKLPGVGPKTAEVIVGVGFNKPSFPVDTHVFRVARRLGLSKARTPEGVS
FDLKKIFPPNSWIDLHHRLIFFGRRICKAQKPSCNICPFPEFCQKEESDV
>Mature_209_residues
TGKKAHKIAAELEKLFPVAKTELNFQNIFQLLVAVVLSAQSTDRQVNKVTEKLFLFVKEPRDLLDMGEEELSRQIRSLGL
YRNKARNLIKIAEILDREYHGQVPDSFAELLKLPGVGPKTAEVIVGVGFNKPSFPVDTHVFRVARRLGLSKARTPEGVSF
DLKKIFPPNSWIDLHHRLIFFGRRICKAQKPSCNICPFPEFCQKEESDV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=101, Evalue=6e-22,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=41.7061611374408, Blast_Score=167, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=34.3195266272189, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=193, Percent_Identity=27.4611398963731, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=27.0114942528736, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=34.4632768361582, Blast_Score=110, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23886; Mature: 23755

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKKAHKIAAELEKLFPVAKTELNFQNIFQLLVAVVLSAQSTDRQVNKVTEKLFLFVKE
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
PRDLLDMGEEELSRQIRSLGLYRNKARNLIKIAEILDREYHGQVPDSFAELLKLPGVGPK
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TAEVIVGVGFNKPSFPVDTHVFRVARRLGLSKARTPEGVSFDLKKIFPPNSWIDLHHRLI
CHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
FFGRRICKAQKPSCNICPFPEFCQKEESDV
HHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
TGKKAHKIAAELEKLFPVAKTELNFQNIFQLLVAVVLSAQSTDRQVNKVTEKLFLFVKE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
PRDLLDMGEEELSRQIRSLGLYRNKARNLIKIAEILDREYHGQVPDSFAELLKLPGVGPK
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TAEVIVGVGFNKPSFPVDTHVFRVARRLGLSKARTPEGVSFDLKKIFPPNSWIDLHHRLI
CHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
FFGRRICKAQKPSCNICPFPEFCQKEESDV
HHHHHHHHCCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA