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The map label for this gene is rnjA [H]

Identifier: 78043054

GI number: 78043054

Start: 1029386

End: 1031050

Strand: Direct

Name: rnjA [H]

Synonym: CHY_1157

Alternate gene names: 78043054

Gene position: 1029386-1031050 (Clockwise)

Preceding gene: 78044485

Following gene: 78045214

Centisome position: 42.86

GC content: 43.54

Gene sequence:

>1665_bases
TTGCCAATTAAAGATGGAAGACTTCAAATTATACCATTAGGCGGCCTTGGAGAAATCGGGAAAAACATGATGGTCATCAA
ATATAACGATGCCATTATAGTGATCGATGCCGGACTGATGTTTCCCGAAGAAGAACTGTTAGGTATTGACATGGTTATTC
CCGACATGTCTTATCTCATAGAAAACAAAGAAAAAGTTAAAGCGGTGCTTTTAACCCATGGTCACGAGGACCACATTGGT
GGGATGCCTTATTTTTTGAAACAATTTGATGTTCCGGTTTACGGAACAAGGCTGACTTTGGGGCTTTTAAGTGCCAAGCT
TAAAGAAGCGGGGATTCCCAGAGCCAGCTTAAATGTTGTGGCTCCGCGGGATGTTTTAAATATTGGGCCGTTTAAGATTG
AATTTATCAAAGTTTCTCACAGTATACCGGATACTGTAGGAATTGCGGTACATACTCCTGTGGGGACAGTAGTCCACACC
GGCGATTTTAAACTGGATCCTACTCCAGTTGATGGGAAGGTTACGGATTTTTATAAGTTAGCGGAACTTGGAGAAAAAGG
CGTTTTAGTTTTAATGTCGGACAGCACCAATGCCGAAAGGCCCGGCTTTACCTTGTCGGAAAAAACCGTGGGTAATACCT
TTGAAGAAACGTTCAGGGTGGCGGAAGGTAGAATTATCATTGCAACTTTTGCCTCAAATGTTCATCGTTTGCAGCAAGCA
ATTTATACCGCCCATAAATTTAAAAGAAAAGTGGCCATTGTTGGCCGAAGCATGTTGAATGTGGTAGAAATTGCCAACGA
GCTGGGCTATCTGGATATTCCCGATGGACTGTTAATTGATGTGGATGAAATAAACCGCTACCCTCATAAACAAGTCGTTA
TACTAACTACGGGAAGCCAGGGAGAACCAATGTCAGCGCTTACCCGCATGGCCATGTCTGAGCACCGTCAGGTGGAAATT
GTAGCAGGAGATACGGTTATCATTTCTGCAATGCCGATTCCGGGAAATGAGAAGTTGGTCTCCAGAATTATTGACCAGTT
ATTTAAATTGGGTGCCAAAGTTATTTACGAGGCGGTTTCAGGGATTCATGTCTCTGGACACCCCAGCCAGGAAGAATTAA
AATTAATGATCAACCTTTTAAAACCAAAATACTTTGTTCCCATTCACGGAGAATACCGCCATTTAATTAAGCATGCGGAA
ATTGCGCGGGAGTTAGGCATAAAACCCCAAAACATCTTTGTGGTGGAGAACGGTCAGGTTTTGGAATTCACGAAAAAATC
CGGACGTCTTGCCGGAAAAGTACCGGCCGGCCGGGTATTGGTTGATGGACTCGGAGTGGGGGATGTCGGGAATATTGTGT
TAAGGGACCGTAAACAACTGGCCCAGGATGGTATTATTATTGTCGTGCTTACTTTAAACCGGGAGTCCGGAGAGATTGTT
GCCGGTCCAGACATTGTAACCCGGGGATTTGTCTACGTCCGGGAGTCGGAACAGTTAATCGATGAGGCCAAACAGAAGGT
TACCGAAGCTTTAAATAAATGTTTTAATCACGGCTTGTTAGAGTGGTCTGCCATAAAAAGTCAGGTTAGAGATGCGTTAG
GAAAATATCTATTTGAAAAGACCCGCCGTCGTCCAATGATTTTACCGATTATAATGGAAATTTAG

Upstream 100 bases:

>100_bases
CACTTGTCCTTCCTTTTTTTATTTTTTATAATTACAATAGATTGTAAAAAGCTAACAAATGGGCGGTTTAATATAAATCA
AAAATTAAGCAGGAGGTGTA

Downstream 100 bases:

>100_bases
AAAGGGAGAGGACATTGAACTCATTTCAAGCTTTAATTTTAGGACTTGTTCAGGGTTTAACCGAGTATTTACCCGTTTCC
AGTTCCGGGCATTTAGTTTT

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MPIKDGRLQIIPLGGLGEIGKNMMVIKYNDAIIVIDAGLMFPEEELLGIDMVIPDMSYLIENKEKVKAVLLTHGHEDHIG
GMPYFLKQFDVPVYGTRLTLGLLSAKLKEAGIPRASLNVVAPRDVLNIGPFKIEFIKVSHSIPDTVGIAVHTPVGTVVHT
GDFKLDPTPVDGKVTDFYKLAELGEKGVLVLMSDSTNAERPGFTLSEKTVGNTFEETFRVAEGRIIIATFASNVHRLQQA
IYTAHKFKRKVAIVGRSMLNVVEIANELGYLDIPDGLLIDVDEINRYPHKQVVILTTGSQGEPMSALTRMAMSEHRQVEI
VAGDTVIISAMPIPGNEKLVSRIIDQLFKLGAKVIYEAVSGIHVSGHPSQEELKLMINLLKPKYFVPIHGEYRHLIKHAE
IARELGIKPQNIFVVENGQVLEFTKKSGRLAGKVPAGRVLVDGLGVGDVGNIVLRDRKQLAQDGIIIVVLTLNRESGEIV
AGPDIVTRGFVYVRESEQLIDEAKQKVTEALNKCFNHGLLEWSAIKSQVRDALGKYLFEKTRRRPMILPIIMEI

Sequences:

>Translated_554_residues
MPIKDGRLQIIPLGGLGEIGKNMMVIKYNDAIIVIDAGLMFPEEELLGIDMVIPDMSYLIENKEKVKAVLLTHGHEDHIG
GMPYFLKQFDVPVYGTRLTLGLLSAKLKEAGIPRASLNVVAPRDVLNIGPFKIEFIKVSHSIPDTVGIAVHTPVGTVVHT
GDFKLDPTPVDGKVTDFYKLAELGEKGVLVLMSDSTNAERPGFTLSEKTVGNTFEETFRVAEGRIIIATFASNVHRLQQA
IYTAHKFKRKVAIVGRSMLNVVEIANELGYLDIPDGLLIDVDEINRYPHKQVVILTTGSQGEPMSALTRMAMSEHRQVEI
VAGDTVIISAMPIPGNEKLVSRIIDQLFKLGAKVIYEAVSGIHVSGHPSQEELKLMINLLKPKYFVPIHGEYRHLIKHAE
IARELGIKPQNIFVVENGQVLEFTKKSGRLAGKVPAGRVLVDGLGVGDVGNIVLRDRKQLAQDGIIIVVLTLNRESGEIV
AGPDIVTRGFVYVRESEQLIDEAKQKVTEALNKCFNHGLLEWSAIKSQVRDALGKYLFEKTRRRPMILPIIMEI
>Mature_553_residues
PIKDGRLQIIPLGGLGEIGKNMMVIKYNDAIIVIDAGLMFPEEELLGIDMVIPDMSYLIENKEKVKAVLLTHGHEDHIGG
MPYFLKQFDVPVYGTRLTLGLLSAKLKEAGIPRASLNVVAPRDVLNIGPFKIEFIKVSHSIPDTVGIAVHTPVGTVVHTG
DFKLDPTPVDGKVTDFYKLAELGEKGVLVLMSDSTNAERPGFTLSEKTVGNTFEETFRVAEGRIIIATFASNVHRLQQAI
YTAHKFKRKVAIVGRSMLNVVEIANELGYLDIPDGLLIDVDEINRYPHKQVVILTTGSQGEPMSALTRMAMSEHRQVEIV
AGDTVIISAMPIPGNEKLVSRIIDQLFKLGAKVIYEAVSGIHVSGHPSQEELKLMINLLKPKYFVPIHGEYRHLIKHAEI
ARELGIKPQNIFVVENGQVLEFTKKSGRLAGKVPAGRVLVDGLGVGDVGNIVLRDRKQLAQDGIIIVVLTLNRESGEIVA
GPDIVTRGFVYVRESEQLIDEAKQKVTEALNKCFNHGLLEWSAIKSQVRDALGKYLFEKTRRRPMILPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61178; Mature: 61046

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKDGRLQIIPLGGLGEIGKNMMVIKYNDAIIVIDAGLMFPEEELLGIDMVIPDMSYLI
CCCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCHHHHCEEEEECCCHHHHH
ENKEKVKAVLLTHGHEDHIGGMPYFLKQFDVPVYGTRLTLGLLSAKLKEAGIPRASLNVV
CCHHHEEEEEEECCCCCCCCCCCHHHHHCCCCEECHHHHHHHHHHHHHHCCCCCCCEEEE
APRDVLNIGPFKIEFIKVSHSIPDTVGIAVHTPVGTVVHTGDFKLDPTPVDGKVTDFYKL
CCHHHCCCCCEEEEEEEEECCCCCCCCEEEECCCCEEEECCCEEECCCCCCCCHHHHHHH
AELGEKGVLVLMSDSTNAERPGFTLSEKTVGNTFEETFRVAEGRIIIATFASNVHRLQQA
HHCCCCCEEEEEECCCCCCCCCCEECCHHCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHH
IYTAHKFKRKVAIVGRSMLNVVEIANELGYLDIPDGLLIDVDEINRYPHKQVVILTTGSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHHCCCCCCEEEEEEECCC
GEPMSALTRMAMSEHRQVEIVAGDTVIISAMPIPGNEKLVSRIIDQLFKLGAKVIYEAVS
CCCHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIHVSGHPSQEELKLMINLLKPKYFVPIHGEYRHLIKHAEIARELGIKPQNIFVVENGQV
CCEECCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LEFTKKSGRLAGKVPAGRVLVDGLGVGDVGNIVLRDRKQLAQDGIIIVVLTLNRESGEIV
EEEECCCCCEEECCCCCEEEEECCCCCCCCHHEEHHHHHHHCCCEEEEEEEECCCCCCEE
AGPDIVTRGFVYVRESEQLIDEAKQKVTEALNKCFNHGLLEWSAIKSQVRDALGKYLFEK
ECCCHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TRRRPMILPIIMEI
HCCCCEEEEEEECC
>Mature Secondary Structure 
PIKDGRLQIIPLGGLGEIGKNMMVIKYNDAIIVIDAGLMFPEEELLGIDMVIPDMSYLI
CCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCHHHHCEEEEECCCHHHHH
ENKEKVKAVLLTHGHEDHIGGMPYFLKQFDVPVYGTRLTLGLLSAKLKEAGIPRASLNVV
CCHHHEEEEEEECCCCCCCCCCCHHHHHCCCCEECHHHHHHHHHHHHHHCCCCCCCEEEE
APRDVLNIGPFKIEFIKVSHSIPDTVGIAVHTPVGTVVHTGDFKLDPTPVDGKVTDFYKL
CCHHHCCCCCEEEEEEEEECCCCCCCCEEEECCCCEEEECCCEEECCCCCCCCHHHHHHH
AELGEKGVLVLMSDSTNAERPGFTLSEKTVGNTFEETFRVAEGRIIIATFASNVHRLQQA
HHCCCCCEEEEEECCCCCCCCCCEECCHHCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHH
IYTAHKFKRKVAIVGRSMLNVVEIANELGYLDIPDGLLIDVDEINRYPHKQVVILTTGSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHHCCCCCCEEEEEEECCC
GEPMSALTRMAMSEHRQVEIVAGDTVIISAMPIPGNEKLVSRIIDQLFKLGAKVIYEAVS
CCCHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIHVSGHPSQEELKLMINLLKPKYFVPIHGEYRHLIKHAEIARELGIKPQNIFVVENGQV
CCEECCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LEFTKKSGRLAGKVPAGRVLVDGLGVGDVGNIVLRDRKQLAQDGIIIVVLTLNRESGEIV
EEEECCCCCEEECCCCCEEEEECCCCCCCCHHEEHHHHHHHCCCEEEEEEEECCCCCCEE
AGPDIVTRGFVYVRESEQLIDEAKQKVTEALNKCFNHGLLEWSAIKSQVRDALGKYLFEK
ECCCHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TRRRPMILPIIMEI
HCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]