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The map label for this gene is xthA1 [H]

Identifier: 77465425

GI number: 77465425

Start: 491472

End: 492239

Strand: Reverse

Name: xthA1 [H]

Synonym: RSP_3423

Alternate gene names: 77465425

Gene position: 492239-491472 (Counterclockwise)

Preceding gene: 77465427

Following gene: 77465420

Centisome position: 52.2

GC content: 67.32

Gene sequence:

>768_bases
ATGAGGATCGCGACCTTCAACGTGAACGGGGTGAACGGCCGGCTTCCGGTGCTGCTGCGCTGGCTCGAGGAGGCGCGCCC
CGACGTCGTCTGCCTGCAGGAGTTGAAGGCGGCCTCCGCGCGCTTTCCGGCCGCCGCGCTGGAAGCTGCGGGTTACGGAG
CGGTGTGGCACGGGCAGAAGAGCTGGAACGGCGTGGCGATCCTCGCGCGCGGCGCCGAGCCGGTGCAGACGCGGCAGGAT
CTGCCGGGCGTGCAGGACGGTGAAAGCCGCTATATCGAGGCGGCGGTGGAGGGCGTTCTCGTGGGCTGCCTCTATCTGCC
GAACGGCAATCCGGCCCCGGGCCCGAAGTTTGCCGCCAAGCTCGCGTGGTTCGACCATCTTCTCAATCATGCCGAGGAGG
TTCTGCGCCACGACGTGCCGGTCGTTCTCGCCGGAGACTTCAACGTGATGCCGACCGATCTCGACGTCCATCGTCCCGAG
CGCTGGCGCGAGGACGCGCTGTGCCGCCCTGAGGTGCGGGCAGCCTTTGCCCGGCTTCTCGCGCAGGGTTGGACAGATAG
CATCCGGCATCTGTATCCCGAGGATCGGATCTATACGTTCTGGGACTATCTCCGAAATGCGTGGGAGCGGGACGCCGGTC
TGCGGCTCGACCATCTGCTGCTGAGCCCGTCCCTTCTGACCCGTCTGAAGGGGGCGGGGGTCGACCGGCATGTCCGCGGC
TGGGAGAAGGCGAGCGATCACGCTCCGGTCTGGATCGCGATTGACTGA

Upstream 100 bases:

>100_bases
CGCGCACAGCCCGCCCCGCCACATCCTGCCGCGATGCGGATGCCCGGACTGGAGCGCATTTTCCTGCGGACTCCCGTCCG
GCGGAAGCTACATCCGGAAC

Downstream 100 bases:

>100_bases
CGGCCCTCTCTCGCGGGCGCTGGCTCTGTCCCGGTCGCTTGGAGCGGCAGGCGGGAGCGGCAAGCGCTGCGCTGATGCGC
AGGACCTTGATCCCCGGACG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRIATFNVNGVNGRLPVLLRWLEEARPDVVCLQELKAASARFPAAALEAAGYGAVWHGQKSWNGVAILARGAEPVQTRQD
LPGVQDGESRYIEAAVEGVLVGCLYLPNGNPAPGPKFAAKLAWFDHLLNHAEEVLRHDVPVVLAGDFNVMPTDLDVHRPE
RWREDALCRPEVRAAFARLLAQGWTDSIRHLYPEDRIYTFWDYLRNAWERDAGLRLDHLLLSPSLLTRLKGAGVDRHVRG
WEKASDHAPVWIAID

Sequences:

>Translated_255_residues
MRIATFNVNGVNGRLPVLLRWLEEARPDVVCLQELKAASARFPAAALEAAGYGAVWHGQKSWNGVAILARGAEPVQTRQD
LPGVQDGESRYIEAAVEGVLVGCLYLPNGNPAPGPKFAAKLAWFDHLLNHAEEVLRHDVPVVLAGDFNVMPTDLDVHRPE
RWREDALCRPEVRAAFARLLAQGWTDSIRHLYPEDRIYTFWDYLRNAWERDAGLRLDHLLLSPSLLTRLKGAGVDRHVRG
WEKASDHAPVWIAID
>Mature_255_residues
MRIATFNVNGVNGRLPVLLRWLEEARPDVVCLQELKAASARFPAAALEAAGYGAVWHGQKSWNGVAILARGAEPVQTRQD
LPGVQDGESRYIEAAVEGVLVGCLYLPNGNPAPGPKFAAKLAWFDHLLNHAEEVLRHDVPVVLAGDFNVMPTDLDVHRPE
RWREDALCRPEVRAAFARLLAQGWTDSIRHLYPEDRIYTFWDYLRNAWERDAGLRLDHLLLSPSLLTRLKGAGVDRHVRG
WEKASDHAPVWIAID

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=265, Percent_Identity=30.9433962264151, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI18375503, Length=265, Percent_Identity=30.9433962264151, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI18375501, Length=265, Percent_Identity=30.9433962264151, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=36.2264150943396, Blast_Score=154, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=198, Percent_Identity=29.7979797979798, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI221330655, Length=259, Percent_Identity=30.1158301158301, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=30.1158301158301, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28556; Mature: 28556

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATFNVNGVNGRLPVLLRWLEEARPDVVCLQELKAASARFPAAALEAAGYGAVWHGQK
CEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCC
SWNGVAILARGAEPVQTRQDLPGVQDGESRYIEAAVEGVLVGCLYLPNGNPAPGPKFAAK
CCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHH
LAWFDHLLNHAEEVLRHDVPVVLAGDFNVMPTDLDVHRPERWREDALCRPEVRAAFARLL
HHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHH
AQGWTDSIRHLYPEDRIYTFWDYLRNAWERDAGLRLDHLLLSPSLLTRLKGAGVDRHVRG
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCCCHHHCC
WEKASDHAPVWIAID
CCCCCCCCCEEEEEC
>Mature Secondary Structure
MRIATFNVNGVNGRLPVLLRWLEEARPDVVCLQELKAASARFPAAALEAAGYGAVWHGQK
CEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCC
SWNGVAILARGAEPVQTRQDLPGVQDGESRYIEAAVEGVLVGCLYLPNGNPAPGPKFAAK
CCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHH
LAWFDHLLNHAEEVLRHDVPVVLAGDFNVMPTDLDVHRPERWREDALCRPEVRAAFARLL
HHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHH
AQGWTDSIRHLYPEDRIYTFWDYLRNAWERDAGLRLDHLLLSPSLLTRLKGAGVDRHVRG
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCCCHHHCC
WEKASDHAPVWIAID
CCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]