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The map label for this gene is serA [H]

Identifier: 77465409

GI number: 77465409

Start: 473195

End: 474145

Strand: Reverse

Name: serA [H]

Synonym: RSP_3407

Alternate gene names: 77465409

Gene position: 474145-473195 (Counterclockwise)

Preceding gene: 77465410

Following gene: 77465408

Centisome position: 50.28

GC content: 72.34

Gene sequence:

>951_bases
ATGACCGTCGTCCTGACCACCTCGCCGGGCTTCGGCCGCCACGGACGGGTGCCCGCCCGGATCGAGGCCGAGGGCTGGAC
CTTCCTGCGTGCCGCCGACGCGGCCGAGATGGAGGCGCATCTGCCCGAGGCGGATTACCTCGTGGTGGGCCTCGTGCCGG
TGACGGCCGAGGTGCTGGCGAAGGGGCCGCGTCTGAAGGGCGTGCTGAAGCACGGCGTGGGCGTCGACAATATCGACATT
CCGGCCTGCACCGCTGCGGGCCTGCCGGTCACGAACACGCCCGCCGCCAATGCCGATGCCGTGGCGGAACTCGCCATGGG
CCTGATGTTCGCCATGGCGCGCTTCATCCCGCAGGGCCATGCCTCCGTGACCTCGGGCGGCTGGGACCGCCGGATCGGCA
CCCAGCTCGGCGGCAAGGTGCTGGGGATCGTGGGCCTCGGCAACATCGGCAAGCGCCTCGCCCGCCTCGCGCGCGGCCTC
GGCATGGAGGTGCTGGCCACCGACCGGGTCGAGGATCCGGCCTTCGCGCGCGACTGCGGCGTGACCTACCTGCCGCTCGA
GGAGCTTCTGGCCCGCGCCGACTATGTCTCGCTCCATGTCTTCGGCGGGGCGGGGAATGCAGCGCTGATCGACGACCGGG
CGCTGGCGCGGCTGAAGCCCGGCGCGCGGCTCGTCAATCTGGCGCGCGGCGAGGTCGTCGACCTCGACGCGGTGGCCCGG
GCGCTCGAGAGCGGGCAGCTCGGCGGCGTGGCCATCGACGCCTATGTCACCGAGCCGCCGGACGTGTCGCATCCCGTCTT
CGCCCATCCCAACGCCGTCTTCACGCCGCATTCCGGCGCCGACACGCTCGAGGCGCTCGAGAATGTCGGGCTGATGGTGA
TCGAGGACATCCGCACCCTCATCGCCGGCGGACGCCCGGCGCGCTGCCTGAACGCGGCGGAGCTGGGCTGA

Upstream 100 bases:

>100_bases
ACGATCCTTCGGTCGGCCTCTCCCGGCAGCCCGCGCTGCTGCCAGACGCCGCGCAGGACGCGCAGATCCTCGCCATCCTC
ACCGCGCGGGGCCTGAAGGA

Downstream 100 bases:

>100_bases
GCCCGGCCGAACAGGAGAAAGACCCATGTCCAGTTCCCCCCAACCCATCGTCATCACGATGGGCGATCCGTCCGGGGTCG
GCGCCGAAGTGACGGTGAAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding subunit

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTVVLTTSPGFGRHGRVPARIEAEGWTFLRAADAAEMEAHLPEADYLVVGLVPVTAEVLAKGPRLKGVLKHGVGVDNIDI
PACTAAGLPVTNTPAANADAVAELAMGLMFAMARFIPQGHASVTSGGWDRRIGTQLGGKVLGIVGLGNIGKRLARLARGL
GMEVLATDRVEDPAFARDCGVTYLPLEELLARADYVSLHVFGGAGNAALIDDRALARLKPGARLVNLARGEVVDLDAVAR
ALESGQLGGVAIDAYVTEPPDVSHPVFAHPNAVFTPHSGADTLEALENVGLMVIEDIRTLIAGGRPARCLNAAELG

Sequences:

>Translated_316_residues
MTVVLTTSPGFGRHGRVPARIEAEGWTFLRAADAAEMEAHLPEADYLVVGLVPVTAEVLAKGPRLKGVLKHGVGVDNIDI
PACTAAGLPVTNTPAANADAVAELAMGLMFAMARFIPQGHASVTSGGWDRRIGTQLGGKVLGIVGLGNIGKRLARLARGL
GMEVLATDRVEDPAFARDCGVTYLPLEELLARADYVSLHVFGGAGNAALIDDRALARLKPGARLVNLARGEVVDLDAVAR
ALESGQLGGVAIDAYVTEPPDVSHPVFAHPNAVFTPHSGADTLEALENVGLMVIEDIRTLIAGGRPARCLNAAELG
>Mature_315_residues
TVVLTTSPGFGRHGRVPARIEAEGWTFLRAADAAEMEAHLPEADYLVVGLVPVTAEVLAKGPRLKGVLKHGVGVDNIDIP
ACTAAGLPVTNTPAANADAVAELAMGLMFAMARFIPQGHASVTSGGWDRRIGTQLGGKVLGIVGLGNIGKRLARLARGLG
MEVLATDRVEDPAFARDCGVTYLPLEELLARADYVSLHVFGGAGNAALIDDRALARLKPGARLVNLARGEVVDLDAVARA
LESGQLGGVAIDAYVTEPPDVSHPVFAHPNAVFTPHSGADTLEALENVGLMVIEDIRTLIAGGRPARCLNAAELG

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=283, Percent_Identity=37.4558303886926, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI61743967, Length=283, Percent_Identity=30.3886925795053, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI4557497, Length=283, Percent_Identity=30.3886925795053, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI6912396, Length=295, Percent_Identity=28.8135593220339, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI145580578, Length=279, Percent_Identity=28.673835125448, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI4557499, Length=279, Percent_Identity=28.673835125448, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI145580575, Length=279, Percent_Identity=28.673835125448, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=294, Percent_Identity=33.6734693877551, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI87082289, Length=273, Percent_Identity=30.03663003663, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787645, Length=264, Percent_Identity=30.3030303030303, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788660, Length=225, Percent_Identity=32.4444444444444, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=35.3383458646617, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25147481, Length=281, Percent_Identity=27.7580071174377, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=304, Percent_Identity=28.9473684210526, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=276, Percent_Identity=29.3478260869565, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=246, Percent_Identity=28.0487804878049, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=272, Percent_Identity=27.2058823529412, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321253, Length=175, Percent_Identity=30.8571428571429, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921140, Length=253, Percent_Identity=41.1067193675889, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24646446, Length=275, Percent_Identity=30.5454545454545, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24646448, Length=275, Percent_Identity=30.5454545454545, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24646452, Length=275, Percent_Identity=30.5454545454545, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24646450, Length=275, Percent_Identity=30.5454545454545, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI62472511, Length=273, Percent_Identity=30.7692307692308, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574286, Length=238, Percent_Identity=29.4117647058824, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585514, Length=241, Percent_Identity=26.9709543568465, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574282, Length=241, Percent_Identity=26.9709543568465, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45552429, Length=241, Percent_Identity=26.9709543568465, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585516, Length=264, Percent_Identity=26.5151515151515, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45551003, Length=241, Percent_Identity=26.9709543568465, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574284, Length=241, Percent_Identity=26.9709543568465, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571528, Length=259, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 32844; Mature: 32713

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVLTTSPGFGRHGRVPARIEAEGWTFLRAADAAEMEAHLPEADYLVVGLVPVTAEVLA
CEEEEECCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHCCCCCCEEEEEEHHHHHHHHH
KGPRLKGVLKHGVGVDNIDIPACTAAGLPVTNTPAANADAVAELAMGLMFAMARFIPQGH
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
ASVTSGGWDRRIGTQLGGKVLGIVGLGNIGKRLARLARGLGMEVLATDRVEDPAFARDCG
CCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCC
VTYLPLEELLARADYVSLHVFGGAGNAALIDDRALARLKPGARLVNLARGEVVDLDAVAR
CEEECHHHHHHHCCEEEEEEECCCCCEEEECCHHHHHCCCCHHEEHHCCCCEECHHHHHH
ALESGQLGGVAIDAYVTEPPDVSHPVFAHPNAVFTPHSGADTLEALENVGLMVIEDIRTL
HHHCCCCCCEEEEEEECCCCCCCCCEEECCCEEECCCCCHHHHHHHHHCCCHHHHHHHHH
IAGGRPARCLNAAELG
HCCCCCHHHCCHHCCC
>Mature Secondary Structure 
TVVLTTSPGFGRHGRVPARIEAEGWTFLRAADAAEMEAHLPEADYLVVGLVPVTAEVLA
EEEEECCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHCCCCCCEEEEEEHHHHHHHHH
KGPRLKGVLKHGVGVDNIDIPACTAAGLPVTNTPAANADAVAELAMGLMFAMARFIPQGH
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
ASVTSGGWDRRIGTQLGGKVLGIVGLGNIGKRLARLARGLGMEVLATDRVEDPAFARDCG
CCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCC
VTYLPLEELLARADYVSLHVFGGAGNAALIDDRALARLKPGARLVNLARGEVVDLDAVAR
CEEECHHHHHHHCCEEEEEEECCCCCEEEECCHHHHHCCCCHHEEHHCCCCEECHHHHHH
ALESGQLGGVAIDAYVTEPPDVSHPVFAHPNAVFTPHSGADTLEALENVGLMVIEDIRTL
HHHCCCCCCEEEEEEECCCCCCCCCEEECCCEEECCCCCHHHHHHHHHCCCHHHHHHHHH
IAGGRPARCLNAAELG
HCCCCCHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]