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The map label for this gene is gltR [H]

Identifier: 77465401

GI number: 77465401

Start: 463741

End: 464631

Strand: Reverse

Name: gltR [H]

Synonym: RSP_3400

Alternate gene names: 77465401

Gene position: 464631-463741 (Counterclockwise)

Preceding gene: 77465405

Following gene: 77465400

Centisome position: 49.27

GC content: 70.03

Gene sequence:

>891_bases
ATGAATCTTCGCTTCATCGAAACCTTCCTCTGGGTGGCGCGTCTGGGCAGCTTCCGCGCCGCGGCCGAGCGGCTGAACAC
AACGCAGGCCGCCGTCTCGAACCGCATCGCCACGCTCGAGGCGGCGCTGGGGCAGGACCTCTTCGAGCGGATGCCGGGCG
GCGTGCGGCTCTCGCCCATCGGGCAGCGCGCCATCGCACCGGCCGAGGATCTGATGCGCAGCGCCATGGAATTCCGCATC
TCTGTCGGCCGCCCCGAACAGCTCCGCTCGACGGTGCGGATCGGCACCATCGACAGCATCGTGCAGGCCTGGCTCCCCCG
CTTCATCGAGCGGGTGCGCGAGCTCTATCCCGGCCTCACCCTCGATCTGAACGTCGATACATCGCGCGGGATCGCGCGCG
AGATCGTCGAGCGCAAGATCGACCTCGCGCTCCTGATGGGGCCGGTGCTCGAGGCGGGCCTGCGCAACGTGGATCTGGGC
AGCATGGACTGCGCCTGGGTCGCGGCGCCGGGCTTCGGGCTCGGCGGGCGCAGGATCGGCCTCGCCGATCTCGTCGACCT
GCCGATCCTCACCTTCTCGCGCAACAGCGACCCGCATGTCTGGCTGATGCGCCAGTTCGAGGAGCTGCGCCTGCCGCAGC
CCGCGATCTCGAACTCGAATTCGCTCTCGGCCATGCTGCGGCTGGCGCTCGAAGGGGTAGGGGTGGCGCTGATGACACGG
CCGGTCGTCGAGGCGGCGCTGGCCTCCGGGGCGCTCGACCTTCTCGACGTGGATCCGCCCTTTCCGCCGCTCGCGCTCCA
TGCGGTCTTCGCCGACCACGGCGACAACCTGCTGCCCGTGGCCCTCTCGGAGGTCGCGGCGCAGGTCGCGCTGGAGGAGC
GGGGGCGATAA

Upstream 100 bases:

>100_bases
GCCGTCGCAAGATCCTCATCTTGAGCCCGGGGCCTGCGCGTGGGACTTCTGGGCGACATGCGGGGCAGGAGAGCGACATG
CAGGGCAGGAGATGGGCACC

Downstream 100 bases:

>100_bases
GTTTTTCTGTTCGGACCCCACAAAAAAGAGTCGTTTGCCACGGAGCGGCCCGACAGGGAGCCTTGAACGGAGAAAAGCAG
GAGGCTCGCATGAGAGTTAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNLRFIETFLWVARLGSFRAAAERLNTTQAAVSNRIATLEAALGQDLFERMPGGVRLSPIGQRAIAPAEDLMRSAMEFRI
SVGRPEQLRSTVRIGTIDSIVQAWLPRFIERVRELYPGLTLDLNVDTSRGIAREIVERKIDLALLMGPVLEAGLRNVDLG
SMDCAWVAAPGFGLGGRRIGLADLVDLPILTFSRNSDPHVWLMRQFEELRLPQPAISNSNSLSAMLRLALEGVGVALMTR
PVVEAALASGALDLLDVDPPFPPLALHAVFADHGDNLLPVALSEVAAQVALEERGR

Sequences:

>Translated_296_residues
MNLRFIETFLWVARLGSFRAAAERLNTTQAAVSNRIATLEAALGQDLFERMPGGVRLSPIGQRAIAPAEDLMRSAMEFRI
SVGRPEQLRSTVRIGTIDSIVQAWLPRFIERVRELYPGLTLDLNVDTSRGIAREIVERKIDLALLMGPVLEAGLRNVDLG
SMDCAWVAAPGFGLGGRRIGLADLVDLPILTFSRNSDPHVWLMRQFEELRLPQPAISNSNSLSAMLRLALEGVGVALMTR
PVVEAALASGALDLLDVDPPFPPLALHAVFADHGDNLLPVALSEVAAQVALEERGR
>Mature_296_residues
MNLRFIETFLWVARLGSFRAAAERLNTTQAAVSNRIATLEAALGQDLFERMPGGVRLSPIGQRAIAPAEDLMRSAMEFRI
SVGRPEQLRSTVRIGTIDSIVQAWLPRFIERVRELYPGLTLDLNVDTSRGIAREIVERKIDLALLMGPVLEAGLRNVDLG
SMDCAWVAAPGFGLGGRRIGLADLVDLPILTFSRNSDPHVWLMRQFEELRLPQPAISNSNSLSAMLRLALEGVGVALMTR
PVVEAALASGALDLLDVDPPFPPLALHAVFADHGDNLLPVALSEVAAQVALEERGR

Specific function: Positive regulator of glutamate biosynthesis (gltAB genes). Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=258, Percent_Identity=28.2945736434109, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=243, Percent_Identity=27.1604938271605, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI48994962, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788481, Length=272, Percent_Identity=25.7352941176471, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=24.0963855421687, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32255; Mature: 32255

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRFIETFLWVARLGSFRAAAERLNTTQAAVSNRIATLEAALGQDLFERMPGGVRLSPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GQRAIAPAEDLMRSAMEFRISVGRPEQLRSTVRIGTIDSIVQAWLPRFIERVRELYPGLT
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
LDLNVDTSRGIAREIVERKIDLALLMGPVLEAGLRNVDLGSMDCAWVAAPGFGLGGRRIG
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
LADLVDLPILTFSRNSDPHVWLMRQFEELRLPQPAISNSNSLSAMLRLALEGVGVALMTR
HHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
PVVEAALASGALDLLDVDPPFPPLALHAVFADHGDNLLPVALSEVAAQVALEERGR
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLRFIETFLWVARLGSFRAAAERLNTTQAAVSNRIATLEAALGQDLFERMPGGVRLSPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GQRAIAPAEDLMRSAMEFRISVGRPEQLRSTVRIGTIDSIVQAWLPRFIERVRELYPGLT
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
LDLNVDTSRGIAREIVERKIDLALLMGPVLEAGLRNVDLGSMDCAWVAAPGFGLGGRRIG
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
LADLVDLPILTFSRNSDPHVWLMRQFEELRLPQPAISNSNSLSAMLRLALEGVGVALMTR
HHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
PVVEAALASGALDLLDVDPPFPPLALHAVFADHGDNLLPVALSEVAAQVALEERGR
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]