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The map label for this gene is mltB [H]

Identifier: 77465241

GI number: 77465241

Start: 287499

End: 288887

Strand: Reverse

Name: mltB [H]

Synonym: RSP_3229

Alternate gene names: 77465241

Gene position: 288887-287499 (Counterclockwise)

Preceding gene: 77465242

Following gene: 77465233

Centisome position: 30.63

GC content: 72.79

Gene sequence:

>1389_bases
ATGGGCCGACAGGATCGGCGGCAGTTCAGGCCGCGGGGCCTCGCTCAGGCGCCGCCTCGGCAGGGCGCCGCCGAGCCAAT
TGCGCCTGCGCCCGTTTCCGTTAGGCTCCGGTTCGAGGCAGGATCACAAGAACCCGGAGGAAACGGAATGCAGGCGACGC
GGCGCGCGGTGATGCTCGGACTGTTGGCGACGGGGCTCGCCGGCTGCGGCGGCGGCTTCGGGATCGGTTCCGGCGCGGCG
CCGGAGCCCGCGATGCGCGCCGTGCCGAACCCGGCCTGGGACGCCTGGGTGGCGGGCTTCCGGCCGCGTGCGCTCGGCGC
GGGCATCGACCCCGGCACCTTCGACCGCGCCTTTCGCTCGGCGGGCTTCCTGCCCGACGTGATCGAGCGCGACCGCAACC
AGACCGAATTCACCCGCACGACCGAGGATTACCTCTCGATCGCCGCCTCCGAGGAGCGGGTGTCGCTGGGTCGCGCGGCC
CTCGCGCGTCATGCGGGCGTGCTCTCGCAGATCGAATCCCGCTACGGGGTCGAGGCCGAGGTCGTGACCGCCGTCTGGGG
CGTCGAGAGCCGCTATGGCGAGCGGCGGGGCGACGTGCCGATCGTCTCGGCCCTCTCCACCCTCGCCTTCGAGGGCCGCC
GCGGCGCCTTCTTCGAAAGCCAGCTGATCGCCGCGCTGAAGATCCTGCAGTCGGGCGACACCGATCCCGCCCATCTGACC
GGCAGCTGGGCGGGCGCGATGGGGCACACCCAGTTCATCCCGACCTCGTATCTGGCCTATGCGGTCGATTTCAACGGCGA
CGGCCGCCGCGACATCTGGTCGGACGATCCGACGGACGCGCTTGCCTCGGCCGCGGCCTATCTGTCGCGCTCGGGCTGGC
GGCGCGGTCAGCCGTGGGGTGTGGAGGTGCAGCTTCCGGCCGGGTTCAACAGCGGCCTCGCGGGGCGCGGCACCACGCGC
AGCCCGGCGGCCTGGACGGCCATGGGCGTGCGCGACATGGACGGGCGCGCGGTGCCCGACCACGGCGCGGCTTCCATCCT
CATCCCGATGGGGCTCGCCGGACCCGCCTTCATGGTCTTCCGCAACTTCACCGTCATCACCCGCTACAACAATGCCGAGA
ACTACGTCATCGGCGTGGGGCACCTGTCGGACCGTCTCCGCGGCGGGCCGCCGATCCGGGGCCGCTTCCCGCCGGACGCC
CGCGGCATGACCAAGGCCGACCGGCAGGAGCTGCAGCGCCGCCTGACCTCGGCGGGCTACGACACCGGCGGAACCGACGG
GGTGGTGGGAAGCAAGACCGAGGCCGCGATCTCGGCTTACGAGGCGGCGAACGGCCTGCCGGTCACCGGCCAGCCCTCGG
TTCCGCTCCTGCGCCGGCTGGGCGGCTGA

Upstream 100 bases:

>100_bases
GCAGAAGCTCAACCGCGCGCTTTGATCCGATTGCGCCCCTCGGGCAGCCGTGCAGGCTGGGCCTTCGCTTGCTCTTGTCC
CGCGAAAGGCGCTCGCGTCC

Downstream 100 bases:

>100_bases
GGGCGGGCCTCCCCGCCCCGCAAAGCCGCGCGTCTCAGGGCACGAGAGCCTCGAGCTCGGGCAGGAGCGTCACGCTCTCC
TGCTCGTTCGGATCGGTGCG

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MGRQDRRQFRPRGLAQAPPRQGAAEPIAPAPVSVRLRFEAGSQEPGGNGMQATRRAVMLGLLATGLAGCGGGFGIGSGAA
PEPAMRAVPNPAWDAWVAGFRPRALGAGIDPGTFDRAFRSAGFLPDVIERDRNQTEFTRTTEDYLSIAASEERVSLGRAA
LARHAGVLSQIESRYGVEAEVVTAVWGVESRYGERRGDVPIVSALSTLAFEGRRGAFFESQLIAALKILQSGDTDPAHLT
GSWAGAMGHTQFIPTSYLAYAVDFNGDGRRDIWSDDPTDALASAAAYLSRSGWRRGQPWGVEVQLPAGFNSGLAGRGTTR
SPAAWTAMGVRDMDGRAVPDHGAASILIPMGLAGPAFMVFRNFTVITRYNNAENYVIGVGHLSDRLRGGPPIRGRFPPDA
RGMTKADRQELQRRLTSAGYDTGGTDGVVGSKTEAAISAYEAANGLPVTGQPSVPLLRRLGG

Sequences:

>Translated_462_residues
MGRQDRRQFRPRGLAQAPPRQGAAEPIAPAPVSVRLRFEAGSQEPGGNGMQATRRAVMLGLLATGLAGCGGGFGIGSGAA
PEPAMRAVPNPAWDAWVAGFRPRALGAGIDPGTFDRAFRSAGFLPDVIERDRNQTEFTRTTEDYLSIAASEERVSLGRAA
LARHAGVLSQIESRYGVEAEVVTAVWGVESRYGERRGDVPIVSALSTLAFEGRRGAFFESQLIAALKILQSGDTDPAHLT
GSWAGAMGHTQFIPTSYLAYAVDFNGDGRRDIWSDDPTDALASAAAYLSRSGWRRGQPWGVEVQLPAGFNSGLAGRGTTR
SPAAWTAMGVRDMDGRAVPDHGAASILIPMGLAGPAFMVFRNFTVITRYNNAENYVIGVGHLSDRLRGGPPIRGRFPPDA
RGMTKADRQELQRRLTSAGYDTGGTDGVVGSKTEAAISAYEAANGLPVTGQPSVPLLRRLGG
>Mature_461_residues
GRQDRRQFRPRGLAQAPPRQGAAEPIAPAPVSVRLRFEAGSQEPGGNGMQATRRAVMLGLLATGLAGCGGGFGIGSGAAP
EPAMRAVPNPAWDAWVAGFRPRALGAGIDPGTFDRAFRSAGFLPDVIERDRNQTEFTRTTEDYLSIAASEERVSLGRAAL
ARHAGVLSQIESRYGVEAEVVTAVWGVESRYGERRGDVPIVSALSTLAFEGRRGAFFESQLIAALKILQSGDTDPAHLTG
SWAGAMGHTQFIPTSYLAYAVDFNGDGRRDIWSDDPTDALASAAAYLSRSGWRRGQPWGVEVQLPAGFNSGLAGRGTTRS
PAAWTAMGVRDMDGRAVPDHGAASILIPMGLAGPAFMVFRNFTVITRYNNAENYVIGVGHLSDRLRGGPPIRGRFPPDAR
GMTKADRQELQRRLTSAGYDTGGTDGVVGSKTEAAISAYEAANGLPVTGQPSVPLLRRLGG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=238, Percent_Identity=34.453781512605, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 48933; Mature: 48801

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRQDRRQFRPRGLAQAPPRQGAAEPIAPAPVSVRLRFEAGSQEPGGNGMQATRRAVMLG
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHH
LLATGLAGCGGGFGIGSGAAPEPAMRAVPNPAWDAWVAGFRPRALGAGIDPGTFDRAFRS
HHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCHHCCCCCCCCHHHHHHHH
AGFLPDVIERDRNQTEFTRTTEDYLSIAASEERVSLGRAALARHAGVLSQIESRYGVEAE
CCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VVTAVWGVESRYGERRGDVPIVSALSTLAFEGRRGAFFESQLIAALKILQSGDTDPAHLT
EHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEC
GSWAGAMGHTQFIPTSYLAYAVDFNGDGRRDIWSDDPTDALASAAAYLSRSGWRRGQPWG
CCHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
VEVQLPAGFNSGLAGRGTTRSPAAWTAMGVRDMDGRAVPDHGAASILIPMGLAGPAFMVF
EEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
RNFTVITRYNNAENYVIGVGHLSDRLRGGPPIRGRFPPDARGMTKADRQELQRRLTSAGY
HCEEEEEEECCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DTGGTDGVVGSKTEAAISAYEAANGLPVTGQPSVPLLRRLGG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
GRQDRRQFRPRGLAQAPPRQGAAEPIAPAPVSVRLRFEAGSQEPGGNGMQATRRAVMLG
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHH
LLATGLAGCGGGFGIGSGAAPEPAMRAVPNPAWDAWVAGFRPRALGAGIDPGTFDRAFRS
HHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCHHCCCCCCCCHHHHHHHH
AGFLPDVIERDRNQTEFTRTTEDYLSIAASEERVSLGRAALARHAGVLSQIESRYGVEAE
CCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VVTAVWGVESRYGERRGDVPIVSALSTLAFEGRRGAFFESQLIAALKILQSGDTDPAHLT
EHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEC
GSWAGAMGHTQFIPTSYLAYAVDFNGDGRRDIWSDDPTDALASAAAYLSRSGWRRGQPWG
CCHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
VEVQLPAGFNSGLAGRGTTRSPAAWTAMGVRDMDGRAVPDHGAASILIPMGLAGPAFMVF
EEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
RNFTVITRYNNAENYVIGVGHLSDRLRGGPPIRGRFPPDARGMTKADRQELQRRLTSAGY
HCEEEEEEECCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DTGGTDGVVGSKTEAAISAYEAANGLPVTGQPSVPLLRRLGG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]