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The map label for this gene is rstB [H]

Identifier: 77461923

GI number: 77461923

Start: 6393049

End: 6394356

Strand: Reverse

Name: rstB [H]

Synonym: Pfl01_5703

Alternate gene names: 77461923

Gene position: 6394356-6393049 (Counterclockwise)

Preceding gene: 77461924

Following gene: 77461922

Centisome position: 99.32

GC content: 61.93

Gene sequence:

>1308_bases
ATGAATCTCGCTCTGCGCTGGCCGCGTACCCTTGCCTCCAGATTATCGCTGATATTCCTGATCGGCCTGATCCTCGCTCA
GGCGCTGTCATTTAGCGCGCAATATTACGAGCGCTATGAAAGCGCGAAGGACGCGATGCTAGGCAACCTTGAAACCGACG
TTTCCACCTCGATTGCCATCCTCGACCGACTGCCGGCCGAAGAGCGCCCGATGTGGCTCAAACGCCTGGCTCGCAAGAAT
TACGGCTATCTGCTGAGCGGAGGCGAACCGGGCACACCACTGGATCCGCAGGACGTGCCGATTGCCGTCACCTCGATTAC
CGACGCAATTGGCGAGGCCTATCCGCTGACGTTCACCGATATTCCCGGCCCGAAAAAACACTTTCAGGGGCACCTGCGCC
TGAGCGACGGCAGCCCGGTGACCATCGATGTGCGCCCGTCGATGGTGCCGCTGTCGCCGTGGCTGCCGGTGGTATTGCTC
GGGCAACTGGCGCTGATGCTCGCCTGCACCTGGCTGGCGGTGCGTATCGCCATCCGCCCGCTCACCCGCCTCGCCAACGC
CGTGGAAACCCTCGACCCCAACACCAACGCCATACAGCTCGACGAAAAAGGTCCGAACGAGGTGGTCTACGCCGTTCGTG
CGTTCAACGCGATGCAGGCGCGCATCACCACTTATCTGAAGGAGCGCATGCAATTGCTGGCGGCGATCTCCCACGACCTG
CAAACCCCGATCACCCGCATGAAGCTGCGGGCGGAGTTCATGGACGATTCGGTCGAGAAAGACAAACTGTGGAACGACCT
CGGCGAGATGGAACATCTGGTGCGCGAAGGTGTGGCCTATGCCCGCAGCATCCACGGCTCGACCGAGGAAAGCCGACGCA
CCAATCTGGATTCATTCCTCGACAGCCTGGTGTTCGACTATCAGGACATGGGTAAGGAAGTACAGTTGAACGGCAAGAGT
GACGCGGTCATCGACACCCGTCCCCACGCGTTGCGCCGGGTGCTGGTCAACCTGACCGACAACGCGCTGAAGTTCGCCGG
CGCTGCCGAGCTGCTGATTCAACGGGACAGGAACGGCCTCTCGATCAAGGTCATGGATCGCGGCCCGGGCATCGCCGAAG
AAGAGCTGGCCCAGGTGATGGAGCCCTTCTACCGCGTGGAAAACTCGCGCAACCGCAGCACCGGCGGCACCGGGCTGGGG
CTGGCGATCGCCCAGCAACTGGCGCTGGCGCTGGGGGGTTCACTGACGCTGAGCAATCGCGACGGTGGTGGGTTGTGTGC
AGAACTGAGGCTGCCGCTGGAAAGTTAA

Upstream 100 bases:

>100_bases
AGCGCCTGCTGGATGACGCCCGTGAACCGGCCTACATCAAGACCGTACGCAGCGAAGGCTATGTGTTCTCGCTACCGGTC
GAAATCCTTGGAGCTCCCGC

Downstream 100 bases:

>100_bases
ACTAGGGATGAGTCTGTAGGCGTAGGTCTGCTTGGGACAGCGTTGAACCTCCCGGTGAATGCTGAAGCTGGTTGTGCAGA
CTTCAACGTATTGTCGGGCA

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNLALRWPRTLASRLSLIFLIGLILAQALSFSAQYYERYESAKDAMLGNLETDVSTSIAILDRLPAEERPMWLKRLARKN
YGYLLSGGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTDIPGPKKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLL
GQLALMLACTWLAVRIAIRPLTRLANAVETLDPNTNAIQLDEKGPNEVVYAVRAFNAMQARITTYLKERMQLLAAISHDL
QTPITRMKLRAEFMDDSVEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFLDSLVFDYQDMGKEVQLNGKS
DAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDRNGLSIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLG
LAIAQQLALALGGSLTLSNRDGGGLCAELRLPLES

Sequences:

>Translated_435_residues
MNLALRWPRTLASRLSLIFLIGLILAQALSFSAQYYERYESAKDAMLGNLETDVSTSIAILDRLPAEERPMWLKRLARKN
YGYLLSGGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTDIPGPKKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLL
GQLALMLACTWLAVRIAIRPLTRLANAVETLDPNTNAIQLDEKGPNEVVYAVRAFNAMQARITTYLKERMQLLAAISHDL
QTPITRMKLRAEFMDDSVEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFLDSLVFDYQDMGKEVQLNGKS
DAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDRNGLSIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLG
LAIAQQLALALGGSLTLSNRDGGGLCAELRLPLES
>Mature_435_residues
MNLALRWPRTLASRLSLIFLIGLILAQALSFSAQYYERYESAKDAMLGNLETDVSTSIAILDRLPAEERPMWLKRLARKN
YGYLLSGGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTDIPGPKKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLL
GQLALMLACTWLAVRIAIRPLTRLANAVETLDPNTNAIQLDEKGPNEVVYAVRAFNAMQARITTYLKERMQLLAAISHDL
QTPITRMKLRAEFMDDSVEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFLDSLVFDYQDMGKEVQLNGKS
DAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDRNGLSIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLG
LAIAQQLALALGGSLTLSNRDGGGLCAELRLPLES

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=296, Percent_Identity=32.4324324324324, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789808, Length=278, Percent_Identity=29.4964028776978, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790346, Length=266, Percent_Identity=29.3233082706767, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786783, Length=286, Percent_Identity=27.6223776223776, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=270, Percent_Identity=24.8148148148148, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790861, Length=300, Percent_Identity=26.3333333333333, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789149, Length=222, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87082128, Length=301, Percent_Identity=24.9169435215947, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=108, Percent_Identity=38.8888888888889, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=37.037037037037, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=30.0847457627119, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789403, Length=298, Percent_Identity=25.503355704698, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=285, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786600, Length=234, Percent_Identity=26.0683760683761, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=231, Percent_Identity=25.1082251082251, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48059; Mature: 48059

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLALRWPRTLASRLSLIFLIGLILAQALSFSAQYYERYESAKDAMLGNLETDVSTSIAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LDRLPAEERPMWLKRLARKNYGYLLSGGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTD
HHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHCCCCCEEECC
IPGPKKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLLGQLALMLACTWLAVRIAIRP
CCCCHHHCCCEEEECCCCEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRLANAVETLDPNTNAIQLDEKGPNEVVYAVRAFNAMQARITTYLKERMQLLAAISHDL
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTPITRMKLRAEFMDDSVEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFL
HCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
DSLVFDYQDMGKEVQLNGKSDAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDRNGL
HHHHHHHHHCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLGLAIAQQLALALGGSLTLSNR
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
DGGGLCAELRLPLES
CCCCEEEEEECCCCC
>Mature Secondary Structure
MNLALRWPRTLASRLSLIFLIGLILAQALSFSAQYYERYESAKDAMLGNLETDVSTSIAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LDRLPAEERPMWLKRLARKNYGYLLSGGEPGTPLDPQDVPIAVTSITDAIGEAYPLTFTD
HHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHCCCCCEEECC
IPGPKKHFQGHLRLSDGSPVTIDVRPSMVPLSPWLPVVLLGQLALMLACTWLAVRIAIRP
CCCCHHHCCCEEEECCCCEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRLANAVETLDPNTNAIQLDEKGPNEVVYAVRAFNAMQARITTYLKERMQLLAAISHDL
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTPITRMKLRAEFMDDSVEKDKLWNDLGEMEHLVREGVAYARSIHGSTEESRRTNLDSFL
HCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
DSLVFDYQDMGKEVQLNGKSDAVIDTRPHALRRVLVNLTDNALKFAGAAELLIQRDRNGL
HHHHHHHHHCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SIKVMDRGPGIAEEELAQVMEPFYRVENSRNRSTGGTGLGLAIAQQLALALGGSLTLSNR
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
DGGGLCAELRLPLES
CCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]