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The map label for this gene is cfiB [H]

Identifier: 77461860

GI number: 77461860

Start: 6318783

End: 6320228

Strand: Reverse

Name: cfiB [H]

Synonym: Pfl01_5640

Alternate gene names: 77461860

Gene position: 6320228-6318783 (Counterclockwise)

Preceding gene: 77461862

Following gene: 77461859

Centisome position: 98.16

GC content: 62.52

Gene sequence:

>1446_bases
GTGGTGCCACCGGCTCAAGGGAACCTCCAAGTGATAACAAAGATCCTGATCGCCAACCGTGGTGAGATTGCCGTACGAAT
CGTGCGTGCCTGCGCCGAGATGGGCATCCGCTCGGTCGCGATCTACTCCGACGCCGACCGGCATGCCTTGCATGTGAAGC
GTGCGGACGAGGCCCACAGCATTGGTGCCGAGCCGCTGGCCGGTTACCTGAACCCGCGCAAGCTGGTGAACCTGGCCGTG
GAAACCGGTTGCGATGCGCTGCACCCCGGCTACGGTTTCCTCTCGGAAAACGCCGAGCTGGCAGACATCTGCGCCGAACG
CGGGATCAAATTCATCGGCCCGTCGGCGGAAGTCATCCGCCGCATGGGCGACAAGACCGAAGCGCGCCGCAGCATGATCA
AGGCCGGCGTGCCGGTCACGCCGGGCACCGAAGGCAACGTCTCCGGCATCGAGGAAGCATTGAGCGAGGGCGACCGCATC
GGTTACCCGGTGATGCTCAAGGCCACTTCCGGTGGTGGCGGTCGCGGTATCCGTCGCTGCAACAGCCGCGAAGAACTCGA
ACAGAACTTCCCTCGCGTCATTTCCGAAGCCACCAAGGCCTTCGGTTCGGCGGAAGTGTTCCTGGAAAAATGCATCGTCA
ATCCCAAGCACATCGAAGCGCAGATACTCGGCGACAGCTTCGGCAACGTCGTGCATCTGTTCGAACGTGACTGCTCGATC
CAGCGCCGTAACCAGAAGCTGATCGAAATCGCCCCGAGCCCGCAGCTGACCCCGGAACAGCGCGCCTACATCGGCGACCT
GTCGGTGCGTGCGGCCAAGGCCGTGGGTTACGAGAACGCCGGCACCGTGGAGTTTTTGCTCGCCGAGGGCGAGGTGTACT
TCATGGAGATGAATACCCGGGTGCAGGTGGAACACACCATCACCGAAGAAATCACCGGGATCGACATCGTCCGCGAACAG
ATCCGCATCGCCTCCGGGCTGCCGCTGTCGGTGAAGCAGGAGGACATCCAGCATCGCGGTTTTGCCCTGCAATTCCGGAT
CAACGCCGAAGACCCGAAGAACAACTTCCTGCCGAGCTTCGGCAAGATCACCCGTTACTACGCCCCCGGCGGACCTGGCG
TGCGTACCGACACGGCGATCTACACCGGCTACACCATTCCGCCGTTCTACGACTCGATGTGCCTGAAACTGGTGGTCTGG
GCACTGACCTGGGAAGAGGCGATGGACCGTGGTCTGCGCGCCCTCGACGACATGCGCCTGCAAGGCGTGAAGACCACCGC
CGCGTACTACCAGGAAATCCTGCGCAACCCGGAATTCCGTAGCGGCCAGTTCAATACCAGCTTCGTCGAAAGCCACCCCG
AACTGACCAACTACTCGATCAAGCGCAAACCCGAAGAGCTGGCCCTGGCCATCGCCGCCGCCATCGCCGCCCACGCAGGC
CTGTGA

Upstream 100 bases:

>100_bases
CCAATTTTTATTCATTTTTGTTAATCCCTTGGCAGCGCTAGTGTGGAGCCGAGACCAGATTGAAACATTTGGTCACGTGC
CGACCTGGGTCTAGCAGGTC

Downstream 100 bases:

>100_bases
GGAATAGAACAATGACTAAAAAGATTTTCGTAACCGACACCATCCTGCGCGACGCCCACCAATCGCTGCTCGCCACCCGC
ATGCGCACCGAAGACATGCT

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: 2-oxoglutarate carboxylase beta subunit [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MVPPAQGNLQVITKILIANRGEIAVRIVRACAEMGIRSVAIYSDADRHALHVKRADEAHSIGAEPLAGYLNPRKLVNLAV
ETGCDALHPGYGFLSENAELADICAERGIKFIGPSAEVIRRMGDKTEARRSMIKAGVPVTPGTEGNVSGIEEALSEGDRI
GYPVMLKATSGGGGRGIRRCNSREELEQNFPRVISEATKAFGSAEVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSI
QRRNQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFLLAEGEVYFMEMNTRVQVEHTITEEITGIDIVREQ
IRIASGLPLSVKQEDIQHRGFALQFRINAEDPKNNFLPSFGKITRYYAPGGPGVRTDTAIYTGYTIPPFYDSMCLKLVVW
ALTWEEAMDRGLRALDDMRLQGVKTTAAYYQEILRNPEFRSGQFNTSFVESHPELTNYSIKRKPEELALAIAAAIAAHAG
L

Sequences:

>Translated_481_residues
MVPPAQGNLQVITKILIANRGEIAVRIVRACAEMGIRSVAIYSDADRHALHVKRADEAHSIGAEPLAGYLNPRKLVNLAV
ETGCDALHPGYGFLSENAELADICAERGIKFIGPSAEVIRRMGDKTEARRSMIKAGVPVTPGTEGNVSGIEEALSEGDRI
GYPVMLKATSGGGGRGIRRCNSREELEQNFPRVISEATKAFGSAEVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSI
QRRNQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFLLAEGEVYFMEMNTRVQVEHTITEEITGIDIVREQ
IRIASGLPLSVKQEDIQHRGFALQFRINAEDPKNNFLPSFGKITRYYAPGGPGVRTDTAIYTGYTIPPFYDSMCLKLVVW
ALTWEEAMDRGLRALDDMRLQGVKTTAAYYQEILRNPEFRSGQFNTSFVESHPELTNYSIKRKPEELALAIAAAIAAHAG
L
>Mature_481_residues
MVPPAQGNLQVITKILIANRGEIAVRIVRACAEMGIRSVAIYSDADRHALHVKRADEAHSIGAEPLAGYLNPRKLVNLAV
ETGCDALHPGYGFLSENAELADICAERGIKFIGPSAEVIRRMGDKTEARRSMIKAGVPVTPGTEGNVSGIEEALSEGDRI
GYPVMLKATSGGGGRGIRRCNSREELEQNFPRVISEATKAFGSAEVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSI
QRRNQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFLLAEGEVYFMEMNTRVQVEHTITEEITGIDIVREQ
IRIASGLPLSVKQEDIQHRGFALQFRINAEDPKNNFLPSFGKITRYYAPGGPGVRTDTAIYTGYTIPPFYDSMCLKLVVW
ALTWEEAMDRGLRALDDMRLQGVKTTAAYYQEILRNPEFRSGQFNTSFVESHPELTNYSIKRKPEELALAIAAAIAAHAG
L

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=451, Percent_Identity=49.2239467849224, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI106049295, Length=451, Percent_Identity=49.2239467849224, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI106049292, Length=451, Percent_Identity=49.2239467849224, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI116805327, Length=444, Percent_Identity=44.5945945945946, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI189095269, Length=481, Percent_Identity=44.6985446985447, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI65506442, Length=474, Percent_Identity=44.9367088607595, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI295821183, Length=474, Percent_Identity=44.9367088607595, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI38679977, Length=506, Percent_Identity=29.6442687747036, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI38679967, Length=506, Percent_Identity=29.6442687747036, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI38679960, Length=506, Percent_Identity=29.6442687747036, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI38679971, Length=506, Percent_Identity=29.6442687747036, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI38679974, Length=506, Percent_Identity=29.6442687747036, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI134142062, Length=508, Percent_Identity=29.9212598425197, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI18105007, Length=238, Percent_Identity=24.7899159663866, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI170295797, Length=232, Percent_Identity=25.4310344827586, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI169790915, Length=232, Percent_Identity=25.4310344827586, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI21361331, Length=232, Percent_Identity=25.4310344827586, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789654, Length=442, Percent_Identity=50.2262443438914, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786216, Length=245, Percent_Identity=24.8979591836735, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562816, Length=454, Percent_Identity=46.9162995594714, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71987519, Length=473, Percent_Identity=44.6088794926004, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17567343, Length=470, Percent_Identity=43.6170212765958, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71997168, Length=464, Percent_Identity=30.3879310344828, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI71997163, Length=464, Percent_Identity=30.3879310344828, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI133931226, Length=516, Percent_Identity=29.2635658914729, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319695, Length=452, Percent_Identity=47.5663716814159, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321376, Length=452, Percent_Identity=47.787610619469, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319685, Length=441, Percent_Identity=40.3628117913832, Blast_Score=333, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6323863, Length=498, Percent_Identity=30.1204819277108, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6324343, Length=547, Percent_Identity=27.7879341864717, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24652212, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652210, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652214, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19921944, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652216, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI281363050, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652224, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652222, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652220, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652218, Length=454, Percent_Identity=48.0176211453744, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651757, Length=469, Percent_Identity=41.5778251599147, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651759, Length=431, Percent_Identity=40.6032482598608, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI161076409, Length=503, Percent_Identity=29.8210735586481, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI161076407, Length=503, Percent_Identity=29.8210735586481, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24586460, Length=503, Percent_Identity=29.8210735586481, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24586458, Length=503, Percent_Identity=29.8210735586481, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.7 [H]

Molecular weight: Translated: 52916; Mature: 52916

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPPAQGNLQVITKILIANRGEIAVRIVRACAEMGIRSVAIYSDADRHALHVKRADEAHS
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHC
IGAEPLAGYLNPRKLVNLAVETGCDALHPGYGFLSENAELADICAERGIKFIGPSAEVIR
CCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHH
RMGDKTEARRSMIKAGVPVTPGTEGNVSGIEEALSEGDRIGYPVMLKATSGGGGRGIRRC
HHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHC
NSREELEQNFPRVISEATKAFGSAEVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSI
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHEEEEEECCCCCHHHHHHHHCCHH
QRRNQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFLLAEGEVYFMEMNTR
HHCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCE
VQVEHTITEEITGIDIVREQIRIASGLPLSVKQEDIQHRGFALQFRINAEDPKNNFLPSF
EEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCH
GKITRYYAPGGPGVRTDTAIYTGYTIPPFYDSMCLKLVVWALTWEEAMDRGLRALDDMRL
HHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGVKTTAAYYQEILRNPEFRSGQFNTSFVESHPELTNYSIKRKPEELALAIAAAIAAHAG
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHCC
L
C
>Mature Secondary Structure
MVPPAQGNLQVITKILIANRGEIAVRIVRACAEMGIRSVAIYSDADRHALHVKRADEAHS
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHC
IGAEPLAGYLNPRKLVNLAVETGCDALHPGYGFLSENAELADICAERGIKFIGPSAEVIR
CCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHH
RMGDKTEARRSMIKAGVPVTPGTEGNVSGIEEALSEGDRIGYPVMLKATSGGGGRGIRRC
HHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHC
NSREELEQNFPRVISEATKAFGSAEVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSI
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHEEEEEECCCCCHHHHHHHHCCHH
QRRNQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFLLAEGEVYFMEMNTR
HHCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCE
VQVEHTITEEITGIDIVREQIRIASGLPLSVKQEDIQHRGFALQFRINAEDPKNNFLPSF
EEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCH
GKITRYYAPGGPGVRTDTAIYTGYTIPPFYDSMCLKLVVWALTWEEAMDRGLRALDDMRL
HHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGVKTTAAYYQEILRNPEFRSGQFNTSFVESHPELTNYSIKRKPEELALAIAAAIAAHAG
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA