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The map label for this gene is pycB [H]

Identifier: 77461859

GI number: 77461859

Start: 6316963

End: 6318771

Strand: Reverse

Name: pycB [H]

Synonym: Pfl01_5639

Alternate gene names: 77461859

Gene position: 6318771-6316963 (Counterclockwise)

Preceding gene: 77461860

Following gene: 77461853

Centisome position: 98.14

GC content: 62.35

Gene sequence:

>1809_bases
ATGACTAAAAAGATTTTCGTAACCGACACCATCCTGCGCGACGCCCACCAATCGCTGCTCGCCACCCGCATGCGCACCGA
AGACATGCTGCCGATCTGCGACAAGCTCGACAAAGTCGGCTACTGGTCGCTGGAATGCTGGGGCGGCGCGACCTTCGACG
CCTGCGTGCGTTTCCTGAAAGAAGACCCGTGGGAGCGTCTGCGCCAACTGCGCGCCGCGCTGCCCAACACCCGTCTACAA
ATGCTCCTGCGCGGGCAGAACCTGCTGGGCTACCGCCACTACAGCGATGACGTGGTGAAGGCGTTCGTCGCCAAGGCCGC
GGTCAACGGCATCGACGTGTTCCGCATCTTCGACGCGATGAACGATGTGCGTAACCTGCGCGTGGCGATCGAAGCGGTGA
AAGCTGCCGGCAAACACGCCCAGGGCACCATCGCTTACACCACCAGTCCGGTGCACACCATCGACGCGTTCGTTGCCCAG
GCCAAGCAAATGGAAGCCATGGGTTGCGACTCGGTGGCGATCAAGGACATGGCCGGTCTGCTGACCCCGTACGCGACCGG
TGAGCTGGTGCGTGCACTGAAAGCCGAGCAGTCGCTGCCAGTGTTCATCCACTCTCACGACACCGCCGGCCTGGCAGCGA
TGTGCCAGCTCAAGGCCATTGAAAACGGTGCCGACCACATCGACACCGCGATCTCCAGCTTCGCGTCGGGCACCAGTCAC
CCTGGCACCGAATCGATGGTTGCTGCGCTGAAAGGCACCGAGTTCGACACCGGTTTGAATCTGGAGCTGCTGCAAGAGAT
TGGCCTGTACTTCTACGCCGTGCGCAAGAAGTACCACCAGTTCGAAAGCGAATTCACCGCCGTCGATACCCGTGTTCAAG
TCAACCAGGTGCCGGGCGGGATGATTTCCAACCTGGCCAACCAGTTGAAAGAGCAGGGCGCTCTGAACCGCATGGGCGAA
GTGCTGGCGGAGATCCCGCGCGTACGTGAAGACCTCGGCTTCCCGCCGCTGGTGACCCCGACTTCGCAGATCGTCGGCAC
CCAGGCGTTCTTCAACGTGTTGGCCGGCGAGCGCTACAAGACCATCACCAACGAAGTGAAGCTTTACCTGCAAGGCGGCT
ACGGCAAGGCGCCGGGCACTGTGAACGAGAAGCTGCGTCGTCAGGCCATTGGCAGCGAAGAGGTGATCGACGTACGCCCG
GCCGATCTGCTCAAGCCGGAAATGACCAAGCTGCGTGCCGATATCGGCGCATTGGCCAAGTCCGAAGAAGACGTGCTCAC
CTTCGCCATGTTCCCGGACATCGGCCGCAAGTTCCTCGAAGAGCGTGCCGCCGGCACTCTCACCCCGGAAGTGCTGCTGC
CGATCCCGGAAGCGGGCAAGGTGGCTTCGGCCGGCGGCGAAGGCGTACCGACCGAGTTCGTCATCGACGTTCACGGCGAG
ACCTACCGTGTGGACATCACCGGTGTCGGCGTCAAGGCTGAAGGCAAGCGTCACTTCTACCTGTCCATCGACGGCATGCC
GGAAGAAGTGGTGTTCGAGCCGCTCAACGAATTCGTCGGCGGCGGCAGCAGCAAGCGCAAGCAGGCTTCGGCACCGGGCC
ACGTCAGCACCACCATGCCGGGCAACATCGTCGATGTGCTGGTCAAGGAAGGCGACACCGTCAAGGCGGGCCAGGCCGTG
CTGATCACCGAAGCGATGAAGATGGAGACCGAAGTCCAGGCCGCGATTGCCGGCAAGGTCACCGCCATCCACGTTGCCAA
GGGCGACCGGGTCAACCCGGGCGAAATCCTGATCGAGATCGAAGGCTGA

Upstream 100 bases:

>100_bases
CCGAACTGACCAACTACTCGATCAAGCGCAAACCCGAAGAGCTGGCCCTGGCCATCGCCGCCGCCATCGCCGCCCACGCA
GGCCTGTGAGGAATAGAACA

Downstream 100 bases:

>100_bases
GATAACGGCCTCGATACAACGCTTTAAACCTCGGGGGAGCATGTGCTCCCCTTTTTTTGCTTTCAGGTTGCTGCCGCAAC
GTGCGGCAGCAGCCAGTCTG

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTKKIFVTDTILRDAHQSLLATRMRTEDMLPICDKLDKVGYWSLECWGGATFDACVRFLKEDPWERLRQLRAALPNTRLQ
MLLRGQNLLGYRHYSDDVVKAFVAKAAVNGIDVFRIFDAMNDVRNLRVAIEAVKAAGKHAQGTIAYTTSPVHTIDAFVAQ
AKQMEAMGCDSVAIKDMAGLLTPYATGELVRALKAEQSLPVFIHSHDTAGLAAMCQLKAIENGADHIDTAISSFASGTSH
PGTESMVAALKGTEFDTGLNLELLQEIGLYFYAVRKKYHQFESEFTAVDTRVQVNQVPGGMISNLANQLKEQGALNRMGE
VLAEIPRVREDLGFPPLVTPTSQIVGTQAFFNVLAGERYKTITNEVKLYLQGGYGKAPGTVNEKLRRQAIGSEEVIDVRP
ADLLKPEMTKLRADIGALAKSEEDVLTFAMFPDIGRKFLEERAAGTLTPEVLLPIPEAGKVASAGGEGVPTEFVIDVHGE
TYRVDITGVGVKAEGKRHFYLSIDGMPEEVVFEPLNEFVGGGSSKRKQASAPGHVSTTMPGNIVDVLVKEGDTVKAGQAV
LITEAMKMETEVQAAIAGKVTAIHVAKGDRVNPGEILIEIEG

Sequences:

>Translated_602_residues
MTKKIFVTDTILRDAHQSLLATRMRTEDMLPICDKLDKVGYWSLECWGGATFDACVRFLKEDPWERLRQLRAALPNTRLQ
MLLRGQNLLGYRHYSDDVVKAFVAKAAVNGIDVFRIFDAMNDVRNLRVAIEAVKAAGKHAQGTIAYTTSPVHTIDAFVAQ
AKQMEAMGCDSVAIKDMAGLLTPYATGELVRALKAEQSLPVFIHSHDTAGLAAMCQLKAIENGADHIDTAISSFASGTSH
PGTESMVAALKGTEFDTGLNLELLQEIGLYFYAVRKKYHQFESEFTAVDTRVQVNQVPGGMISNLANQLKEQGALNRMGE
VLAEIPRVREDLGFPPLVTPTSQIVGTQAFFNVLAGERYKTITNEVKLYLQGGYGKAPGTVNEKLRRQAIGSEEVIDVRP
ADLLKPEMTKLRADIGALAKSEEDVLTFAMFPDIGRKFLEERAAGTLTPEVLLPIPEAGKVASAGGEGVPTEFVIDVHGE
TYRVDITGVGVKAEGKRHFYLSIDGMPEEVVFEPLNEFVGGGSSKRKQASAPGHVSTTMPGNIVDVLVKEGDTVKAGQAV
LITEAMKMETEVQAAIAGKVTAIHVAKGDRVNPGEILIEIEG
>Mature_601_residues
TKKIFVTDTILRDAHQSLLATRMRTEDMLPICDKLDKVGYWSLECWGGATFDACVRFLKEDPWERLRQLRAALPNTRLQM
LLRGQNLLGYRHYSDDVVKAFVAKAAVNGIDVFRIFDAMNDVRNLRVAIEAVKAAGKHAQGTIAYTTSPVHTIDAFVAQA
KQMEAMGCDSVAIKDMAGLLTPYATGELVRALKAEQSLPVFIHSHDTAGLAAMCQLKAIENGADHIDTAISSFASGTSHP
GTESMVAALKGTEFDTGLNLELLQEIGLYFYAVRKKYHQFESEFTAVDTRVQVNQVPGGMISNLANQLKEQGALNRMGEV
LAEIPRVREDLGFPPLVTPTSQIVGTQAFFNVLAGERYKTITNEVKLYLQGGYGKAPGTVNEKLRRQAIGSEEVIDVRPA
DLLKPEMTKLRADIGALAKSEEDVLTFAMFPDIGRKFLEERAAGTLTPEVLLPIPEAGKVASAGGEGVPTEFVIDVHGET
YRVDITGVGVKAEGKRHFYLSIDGMPEEVVFEPLNEFVGGGSSKRKQASAPGHVSTTMPGNIVDVLVKEGDTVKAGQAVL
ITEAMKMETEVQAAIAGKVTAIHVAKGDRVNPGEILIEIEG

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=629, Percent_Identity=31.7965023847377, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI106049295, Length=629, Percent_Identity=31.7965023847377, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI106049292, Length=629, Percent_Identity=31.7965023847377, Blast_Score=293, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17562816, Length=628, Percent_Identity=33.9171974522293, Blast_Score=323, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6321376, Length=624, Percent_Identity=33.6538461538462, Blast_Score=323, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6319695, Length=626, Percent_Identity=33.0670926517572, Blast_Score=315, Evalue=1e-86,
Organism=Drosophila melanogaster, GI281363050, Length=638, Percent_Identity=33.5423197492163, Blast_Score=305, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24652224, Length=638, Percent_Identity=33.5423197492163, Blast_Score=305, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24652222, Length=638, Percent_Identity=33.5423197492163, Blast_Score=305, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24652220, Length=638, Percent_Identity=33.5423197492163, Blast_Score=305, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24652218, Length=638, Percent_Identity=33.5423197492163, Blast_Score=305, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24652212, Length=639, Percent_Identity=33.0203442879499, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24652210, Length=639, Percent_Identity=33.0203442879499, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24652214, Length=639, Percent_Identity=33.0203442879499, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI19921944, Length=639, Percent_Identity=33.0203442879499, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24652216, Length=639, Percent_Identity=33.0203442879499, Blast_Score=304, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 65401; Mature: 65270

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIFVTDTILRDAHQSLLATRMRTEDMLPICDKLDKVGYWSLECWGGATFDACVRFLK
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
EDPWERLRQLRAALPNTRLQMLLRGQNLLGYRHYSDDVVKAFVAKAAVNGIDVFRIFDAM
CCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHH
NDVRNLRVAIEAVKAAGKHAQGTIAYTTSPVHTIDAFVAQAKQMEAMGCDSVAIKDMAGL
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTPYATGELVRALKAEQSLPVFIHSHDTAGLAAMCQLKAIENGADHIDTAISSFASGTSH
HCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
PGTESMVAALKGTEFDTGLNLELLQEIGLYFYAVRKKYHQFESEFTAVDTRVQVNQVPGG
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
MISNLANQLKEQGALNRMGEVLAEIPRVREDLGFPPLVTPTSQIVGTQAFFNVLAGERYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
TITNEVKLYLQGGYGKAPGTVNEKLRRQAIGSEEVIDVRPADLLKPEMTKLRADIGALAK
HHHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHHHC
SEEDVLTFAMFPDIGRKFLEERAAGTLTPEVLLPIPEAGKVASAGGEGVPTEFVIDVHGE
CCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCC
TYRVDITGVGVKAEGKRHFYLSIDGMPEEVVFEPLNEFVGGGSSKRKQASAPGHVSTTMP
EEEEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCC
GNIVDVLVKEGDTVKAGQAVLITEAMKMETEVQAAIAGKVTAIHVAKGDRVNPGEILIEI
CHHHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEE
EG
CC
>Mature Secondary Structure 
TKKIFVTDTILRDAHQSLLATRMRTEDMLPICDKLDKVGYWSLECWGGATFDACVRFLK
CCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
EDPWERLRQLRAALPNTRLQMLLRGQNLLGYRHYSDDVVKAFVAKAAVNGIDVFRIFDAM
CCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHH
NDVRNLRVAIEAVKAAGKHAQGTIAYTTSPVHTIDAFVAQAKQMEAMGCDSVAIKDMAGL
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTPYATGELVRALKAEQSLPVFIHSHDTAGLAAMCQLKAIENGADHIDTAISSFASGTSH
HCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
PGTESMVAALKGTEFDTGLNLELLQEIGLYFYAVRKKYHQFESEFTAVDTRVQVNQVPGG
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
MISNLANQLKEQGALNRMGEVLAEIPRVREDLGFPPLVTPTSQIVGTQAFFNVLAGERYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
TITNEVKLYLQGGYGKAPGTVNEKLRRQAIGSEEVIDVRPADLLKPEMTKLRADIGALAK
HHHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHHHC
SEEDVLTFAMFPDIGRKFLEERAAGTLTPEVLLPIPEAGKVASAGGEGVPTEFVIDVHGE
CCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCC
TYRVDITGVGVKAEGKRHFYLSIDGMPEEVVFEPLNEFVGGGSSKRKQASAPGHVSTTMP
EEEEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCC
GNIVDVLVKEGDTVKAGQAVLITEAMKMETEVQAAIAGKVTAIHVAKGDRVNPGEILIEI
CHHHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEE
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]