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The map label for this gene is dadX [H]

Identifier: 77461745

GI number: 77461745

Start: 6193627

End: 6194724

Strand: Reverse

Name: dadX [H]

Synonym: Pfl01_5524

Alternate gene names: 77461745

Gene position: 6194724-6193627 (Counterclockwise)

Preceding gene: 77461746

Following gene: 77461744

Centisome position: 96.22

GC content: 63.93

Gene sequence:

>1098_bases
ATGACCGCCAAGAAGTCTGCTGCCATGCGTCCTGCCCGTGCCCTGATCGACCTTCAAGCCCTGCGTCACAACTACCGTAT
CGCCCGTGAAGTCACCGGTGCCAAGGCGCTGGCGGTGATCAAGGCCGATGCCTACGGCCATGGCGCGGTACGTTGCGCCC
AGGCGCTGGAGGCTGAAGCGGACGGCTTCGCGGTGGCATGCATCGAAGAAGCCCTGGAGCTGCGTGCCGCCGGGATTCGC
GCGCCAGTGCTGCTGCTCGAAGGCTTTTTCGAGGCCGACGAGCTGGCGCTGATCGTCGAGCACGATTTCTGGTGTGTGGT
GCATTCGCTGTGGCAGCTCGAAGCGATCGAACAGACGACGTTGAGCAAACCGATCACCGTGTGGCTGAAGCTCGATTCGG
GCATGCACCGGGTCGGCCTGCATCCGAAGGATTACCCGGCGGCATACCAGCGTTTGCTGGCCAGCGGCAACGTGGCGAAA
GTCGTGCTGATGAGTCACTTCGCCCGGGCCGATGAATTGCACGAGCAGAGCAGCGCCGATCAGGTTGCAGTGTTCGAAGC
TGCGCGTCAGGGCCTGGCGGCCGAAGTCAGCCTGCGCAATTCGCCGGCGGTGCTGGGCTGGCCACAGATTCACAGCGACT
GGGTGCGCCCGGGCATCATGCTCTACGGCGCAACCCCGTTCGAAGAAGCCAATGCCGTGGCCGAGCGCCTGCAACCGGTG
ATGACCCTGGAGTCGAAAGTCATCAGCGTGCGCGAATTGCCGGCCGGCGAACCGGTGGGTTACGGTGCGAAATTCATCAC
CGACAAACCGATGCGCATCGGCGTGGTTGCCATGGGTTATGCCGACGGTTACCCGCGTCAGGCACCGACCGGCACGCCGG
TGCTGGTGGCCGGCCAGCGCAGCCGCATTCTTGGCCGGGTGTCGATGGACATGCTGTGCATCGACCTGACCGATGTGCCG
CAAGCCGGCCTCGGTTCGACCGTCGAGTTGTGGGGCAAGAACATCCTCGCCAGCGACGTGGCGAAGTGGGCGGACACCAT
TCCGTACCAGATTTTCTGCAACCTGCGGCGGGTGCCAAGGCTCTATTCCGAGGGTTGA

Upstream 100 bases:

>100_bases
AGATCTCCATCGTCGCCGCGTTGCCGTAACCCGTTGTAAAGATCCCTCTCCCGGTGCTGTTGGCGGTTCACGCCGCCAGC
CAGCGCCGGTTTTTATTCCC

Downstream 100 bases:

>100_bases
CGCGCAAGCGGGGCCCAAGTGTTGTAAATACTGAACGCTGTCGCCATGATAACGCTCAATATTTCTACAACATCAGGAGG
CTCCAGCCTTGGACGTCGGT

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTAKKSAAMRPARALIDLQALRHNYRIAREVTGAKALAVIKADAYGHGAVRCAQALEAEADGFAVACIEEALELRAAGIR
APVLLLEGFFEADELALIVEHDFWCVVHSLWQLEAIEQTTLSKPITVWLKLDSGMHRVGLHPKDYPAAYQRLLASGNVAK
VVLMSHFARADELHEQSSADQVAVFEAARQGLAAEVSLRNSPAVLGWPQIHSDWVRPGIMLYGATPFEEANAVAERLQPV
MTLESKVISVRELPAGEPVGYGAKFITDKPMRIGVVAMGYADGYPRQAPTGTPVLVAGQRSRILGRVSMDMLCIDLTDVP
QAGLGSTVELWGKNILASDVAKWADTIPYQIFCNLRRVPRLYSEG

Sequences:

>Translated_365_residues
MTAKKSAAMRPARALIDLQALRHNYRIAREVTGAKALAVIKADAYGHGAVRCAQALEAEADGFAVACIEEALELRAAGIR
APVLLLEGFFEADELALIVEHDFWCVVHSLWQLEAIEQTTLSKPITVWLKLDSGMHRVGLHPKDYPAAYQRLLASGNVAK
VVLMSHFARADELHEQSSADQVAVFEAARQGLAAEVSLRNSPAVLGWPQIHSDWVRPGIMLYGATPFEEANAVAERLQPV
MTLESKVISVRELPAGEPVGYGAKFITDKPMRIGVVAMGYADGYPRQAPTGTPVLVAGQRSRILGRVSMDMLCIDLTDVP
QAGLGSTVELWGKNILASDVAKWADTIPYQIFCNLRRVPRLYSEG
>Mature_364_residues
TAKKSAAMRPARALIDLQALRHNYRIAREVTGAKALAVIKADAYGHGAVRCAQALEAEADGFAVACIEEALELRAAGIRA
PVLLLEGFFEADELALIVEHDFWCVVHSLWQLEAIEQTTLSKPITVWLKLDSGMHRVGLHPKDYPAAYQRLLASGNVAKV
VLMSHFARADELHEQSSADQVAVFEAARQGLAAEVSLRNSPAVLGWPQIHSDWVRPGIMLYGATPFEEANAVAERLQPVM
TLESKVISVRELPAGEPVGYGAKFITDKPMRIGVVAMGYADGYPRQAPTGTPVLVAGQRSRILGRVSMDMLCIDLTDVPQ
AGLGSTVELWGKNILASDVAKWADTIPYQIFCNLRRVPRLYSEG

Specific function: Provides the D-alanine required for cell wall biosynthesis [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=353, Percent_Identity=49.2917847025496, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1790487, Length=350, Percent_Identity=48, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 39775; Mature: 39644

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKKSAAMRPARALIDLQALRHNYRIAREVTGAKALAVIKADAYGHGAVRCAQALEAEA
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCC
DGFAVACIEEALELRAAGIRAPVLLLEGFFEADELALIVEHDFWCVVHSLWQLEAIEQTT
CCEEHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LSKPITVWLKLDSGMHRVGLHPKDYPAAYQRLLASGNVAKVVLMSHFARADELHEQSSAD
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
QVAVFEAARQGLAAEVSLRNSPAVLGWPQIHSDWVRPGIMLYGATPFEEANAVAERLQPV
HHHHHHHHHCCCEEEEEECCCCCEEECHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
MTLESKVISVRELPAGEPVGYGAKFITDKPMRIGVVAMGYADGYPRQAPTGTPVLVAGQR
HHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCH
SRILGRVSMDMLCIDLTDVPQAGLGSTVELWGKNILASDVAKWADTIPYQIFCNLRRVPR
HHHHHHHCCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHCHHHHCH
LYSEG
HHCCC
>Mature Secondary Structure 
TAKKSAAMRPARALIDLQALRHNYRIAREVTGAKALAVIKADAYGHGAVRCAQALEAEA
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCC
DGFAVACIEEALELRAAGIRAPVLLLEGFFEADELALIVEHDFWCVVHSLWQLEAIEQTT
CCEEHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LSKPITVWLKLDSGMHRVGLHPKDYPAAYQRLLASGNVAKVVLMSHFARADELHEQSSAD
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
QVAVFEAARQGLAAEVSLRNSPAVLGWPQIHSDWVRPGIMLYGATPFEEANAVAERLQPV
HHHHHHHHHCCCEEEEEECCCCCEEECHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
MTLESKVISVRELPAGEPVGYGAKFITDKPMRIGVVAMGYADGYPRQAPTGTPVLVAGQR
HHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCH
SRILGRVSMDMLCIDLTDVPQAGLGSTVELWGKNILASDVAKWADTIPYQIFCNLRRVPR
HHHHHHHCCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHCHHHHCH
LYSEG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA