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The map label for this gene is afaC [H]

Identifier: 77459891

GI number: 77459891

Start: 4152073

End: 4154499

Strand: Reverse

Name: afaC [H]

Synonym: Pfl01_3669

Alternate gene names: 77459891

Gene position: 4154499-4152073 (Counterclockwise)

Preceding gene: 77459892

Following gene: 77459890

Centisome position: 64.53

GC content: 62.63

Gene sequence:

>2427_bases
ATGTCGAAGCCTGGCTTCGGCGCCAGCGGTTTCAGGAGGAATCAGTCCATTGACGGACGTAACACGCTAATGAGTCCGGG
ATGGGCTCGCCGTATTCAGCGTCCGTTGTGGCTCATGACCGGCGCGTGGTGCCTGATGTTCATTCATCCATCCGGGGCCG
GCGAACTGCCGCCGCCTCCCAGCGGCATGGAGGCCGTCAGCGATGCACAGTTGTTTCTGGAACTGGTGGTCAACCAGATG
AATACCGGCCGGGTGGTGGCGGTGGAACAGCGCGGCGGGCGCCTGTTCCTGCCAGCCAGCGTATTGCGCGACACCGGCAT
GAAGCTGCCGGAAGAGGCGGGTGCCGAAGTCGATCTCGACGGCCTGCCGGGCCTGCACAGCGATTACGACAGTGTCGGCC
AGCGCCTGCTGCTCGACGTGCCGCCGGACTGGCTGCCGGAGCAGTTCATCGGTAACCGTGAAGTTTATCCACGCACCCCG
GCGCTGAGCAGTTTCGGCGCGTTGTTCAACTATGACCTGTATCTCAATGACACCGACGACGCCGGCACCTATCTGGCGGC
GTGGAACGAGGTGCGGGTGTTCGACAGTTGGGGCACTTTGTCGAACACCGGGCAATATCGCCGCACGTTGTCGGGGGATG
CGGTCAGCACGCTGGACAACGGTTATCTGCGTTACGACACCACCTGGCGTTACTCCGATGACGAACGGATGCTGACCTAC
GAGGCCGGGGACGTGGTCAGCGGCGCCTTGCCCTGGAGCAGTTCGGTGCGGCTGGGCGGTGTGCAGTTCTCCAGGGATTT
TGCGGTACGCCCGGATCTGGTGACCTATCCGCTGCCGCAATTCGCCGGGGAAGCGGCGGTGCCATCTTCGGTGGATCTGT
TCATCAACGGCTACAAATCCAGCAGTACCGACCTGCAACCTGGGCCGTACACCCTGACCAACATTCCGTTTATCAACGGT
GCGGGCGAGGCGGTGGTGGTGACCACCGATGCGCTGGGCCGGCAGGTGTCGACCACGGTGCCGTTCTACGTCACCAGCAG
CCTGTTGCAAAAAGGCCTGAGCGATTTTTCGGTGGCAGCCGGTACGTTGCGCCGGGATTATGGCCTGAAGGATTTTAGCT
ATGGGCCGGGCGTGACCTCGGGCAGTCTGCGTTACGGCGTCAGCGACACCTTTACTCTGGAGAGTCATGCCGAAGCGGCG
GATACCCTGACCCTCGGCGGGCTGGGCGGTAACTTGCGGCTGGGCAATTTCGGGGTGCTCAACAGCGCGATCAGCCAGAG
CCGTTTCGATGGCGACGGTGGTCAGCAACTGAGCCTCGGTTATCAATACAGCGGCCAGCGCTACAGCTTTTCCTATCAGC
GTCTGCAACGCCGCGATCGCTATGCCGACCTGACCGTGGTCGACAGCCCTTACACCACACTCAGCAAGCGCAGCGAGCAG
GCGACCCTGAGCCTCAACCTCGAGCGCTGGGGCAGTCTCGGCGTCGGTTATTTCGATATTCGCGCGGCAGACGAAACCCG
CACGCGGTTGCTCAATCTGAGCTGGAGCAAACCGCTGTGGCGCAACAGCAGTTTCTACCTGTCGGCCAACCGCGAAATCG
GTGACAGCAACTGGGCAGTGCAGGCGCAACTGGTGATTCCGTTCGACCTGCGCGGCAGCCTGGCCATCAGCAGCGAACGC
AGCAAGACCGGACAGACCCAGCAACGGGTCAATTACAGCCGCGCCGTACCGTCCGAGGGCGGGGTGGGCTTCAATCTCGG
CTATGCCAAGGGCGACGGCGCCGATTACCGTCAGGCGGACGTGACCTGGCGCCTGCAATCGGTGCAATTGCAGGCGGGCG
TCTACGGCACGTCCGACGCCGAAACCCGTTGGGCCGATGCCAGCGGTTCGCTGGTGTGGATGGATGATCAAGTGTTCGCC
GCCAACCGCATCGACGATGCGTTCGTGGTGGTCAGCACCGATGGTTATGCGGACATTCCGGTGCGTTACGAGAACCAGCA
GGTCGGCCAGACCGACCGCAACGGCCACCTGCTGGTGCCGTGGAGCAGCGCCTATTACCGCGGCAAATACGAAATCGACC
CGCTGAACCTGCCGGCCAACGTGCGCAGCCCCAACGTCGAACAGCGTATTGCCGTACGCCGGGGCAGTGGCTATCTGCTG
GAATTTCCGCTGAGCCGGGTGATTGCCGCGAGCATCGTGCTGGTGGATGCGCAACAAAAGGAATTGCCGCTGGGCAGTGG
CGTGCTGCACGAGCAGAGCGGCACGCGGACGGTGGTCGGCTGGGACGGGCTGGTCTATCTGGAAAATCTCCAGGCGCAGA
ATTCGCTGCGAGTGACACTTGCGGACGGCAAGACTTGCCAGGCGCAATTCAGTGTCGATCTGCAGCAGGACGAAGTGCCA
CTGATCGGCCCGTTGGTGTGCCAATGA

Upstream 100 bases:

>100_bases
CTGGCCGGCACCGGGGCCGGTGACGGGAGAGCTGGCGGGGCGGATCAATGGCGCGGCAAAGGTGCAGAGCATTCCGCCGG
CGCGATAGCCGTAGCGAGTC

Downstream 100 bases:

>100_bases
TCCGGGCGCGATTTGTTCTGTGGCTGTTGTTGCTGCTGCCGGGGCTGGCGCAGGCGCTGTGTTCGGTGGTGACCACCACA
CCGGCCGGGTTCGGGAACAT

Product: fimbrial biogenesis outer membrane usher protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MSKPGFGASGFRRNQSIDGRNTLMSPGWARRIQRPLWLMTGAWCLMFIHPSGAGELPPPPSGMEAVSDAQLFLELVVNQM
NTGRVVAVEQRGGRLFLPASVLRDTGMKLPEEAGAEVDLDGLPGLHSDYDSVGQRLLLDVPPDWLPEQFIGNREVYPRTP
ALSSFGALFNYDLYLNDTDDAGTYLAAWNEVRVFDSWGTLSNTGQYRRTLSGDAVSTLDNGYLRYDTTWRYSDDERMLTY
EAGDVVSGALPWSSSVRLGGVQFSRDFAVRPDLVTYPLPQFAGEAAVPSSVDLFINGYKSSSTDLQPGPYTLTNIPFING
AGEAVVVTTDALGRQVSTTVPFYVTSSLLQKGLSDFSVAAGTLRRDYGLKDFSYGPGVTSGSLRYGVSDTFTLESHAEAA
DTLTLGGLGGNLRLGNFGVLNSAISQSRFDGDGGQQLSLGYQYSGQRYSFSYQRLQRRDRYADLTVVDSPYTTLSKRSEQ
ATLSLNLERWGSLGVGYFDIRAADETRTRLLNLSWSKPLWRNSSFYLSANREIGDSNWAVQAQLVIPFDLRGSLAISSER
SKTGQTQQRVNYSRAVPSEGGVGFNLGYAKGDGADYRQADVTWRLQSVQLQAGVYGTSDAETRWADASGSLVWMDDQVFA
ANRIDDAFVVVSTDGYADIPVRYENQQVGQTDRNGHLLVPWSSAYYRGKYEIDPLNLPANVRSPNVEQRIAVRRGSGYLL
EFPLSRVIAASIVLVDAQQKELPLGSGVLHEQSGTRTVVGWDGLVYLENLQAQNSLRVTLADGKTCQAQFSVDLQQDEVP
LIGPLVCQ

Sequences:

>Translated_808_residues
MSKPGFGASGFRRNQSIDGRNTLMSPGWARRIQRPLWLMTGAWCLMFIHPSGAGELPPPPSGMEAVSDAQLFLELVVNQM
NTGRVVAVEQRGGRLFLPASVLRDTGMKLPEEAGAEVDLDGLPGLHSDYDSVGQRLLLDVPPDWLPEQFIGNREVYPRTP
ALSSFGALFNYDLYLNDTDDAGTYLAAWNEVRVFDSWGTLSNTGQYRRTLSGDAVSTLDNGYLRYDTTWRYSDDERMLTY
EAGDVVSGALPWSSSVRLGGVQFSRDFAVRPDLVTYPLPQFAGEAAVPSSVDLFINGYKSSSTDLQPGPYTLTNIPFING
AGEAVVVTTDALGRQVSTTVPFYVTSSLLQKGLSDFSVAAGTLRRDYGLKDFSYGPGVTSGSLRYGVSDTFTLESHAEAA
DTLTLGGLGGNLRLGNFGVLNSAISQSRFDGDGGQQLSLGYQYSGQRYSFSYQRLQRRDRYADLTVVDSPYTTLSKRSEQ
ATLSLNLERWGSLGVGYFDIRAADETRTRLLNLSWSKPLWRNSSFYLSANREIGDSNWAVQAQLVIPFDLRGSLAISSER
SKTGQTQQRVNYSRAVPSEGGVGFNLGYAKGDGADYRQADVTWRLQSVQLQAGVYGTSDAETRWADASGSLVWMDDQVFA
ANRIDDAFVVVSTDGYADIPVRYENQQVGQTDRNGHLLVPWSSAYYRGKYEIDPLNLPANVRSPNVEQRIAVRRGSGYLL
EFPLSRVIAASIVLVDAQQKELPLGSGVLHEQSGTRTVVGWDGLVYLENLQAQNSLRVTLADGKTCQAQFSVDLQQDEVP
LIGPLVCQ
>Mature_807_residues
SKPGFGASGFRRNQSIDGRNTLMSPGWARRIQRPLWLMTGAWCLMFIHPSGAGELPPPPSGMEAVSDAQLFLELVVNQMN
TGRVVAVEQRGGRLFLPASVLRDTGMKLPEEAGAEVDLDGLPGLHSDYDSVGQRLLLDVPPDWLPEQFIGNREVYPRTPA
LSSFGALFNYDLYLNDTDDAGTYLAAWNEVRVFDSWGTLSNTGQYRRTLSGDAVSTLDNGYLRYDTTWRYSDDERMLTYE
AGDVVSGALPWSSSVRLGGVQFSRDFAVRPDLVTYPLPQFAGEAAVPSSVDLFINGYKSSSTDLQPGPYTLTNIPFINGA
GEAVVVTTDALGRQVSTTVPFYVTSSLLQKGLSDFSVAAGTLRRDYGLKDFSYGPGVTSGSLRYGVSDTFTLESHAEAAD
TLTLGGLGGNLRLGNFGVLNSAISQSRFDGDGGQQLSLGYQYSGQRYSFSYQRLQRRDRYADLTVVDSPYTTLSKRSEQA
TLSLNLERWGSLGVGYFDIRAADETRTRLLNLSWSKPLWRNSSFYLSANREIGDSNWAVQAQLVIPFDLRGSLAISSERS
KTGQTQQRVNYSRAVPSEGGVGFNLGYAKGDGADYRQADVTWRLQSVQLQAGVYGTSDAETRWADASGSLVWMDDQVFAA
NRIDDAFVVVSTDGYADIPVRYENQQVGQTDRNGHLLVPWSSAYYRGKYEIDPLNLPANVRSPNVEQRIAVRRGSGYLLE
FPLSRVIAASIVLVDAQQKELPLGSGVLHEQSGTRTVVGWDGLVYLENLQAQNSLRVTLADGKTCQAQFSVDLQQDEVPL
IGPLVCQ

Specific function: Involved in the export and assembly of AFA-III afimbrial adhesin subunits across the outer membrane [H]

COG id: COG3188

COG function: function code NU; P pilus assembly protein, porin PapC

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial export usher family [H]

Homologues:

Organism=Escherichia coli, GI1790772, Length=615, Percent_Identity=22.2764227642276, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788427, Length=603, Percent_Identity=23.0514096185738, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1786744, Length=615, Percent_Identity=22.6016260162602, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789533, Length=607, Percent_Identity=21.0873146622735, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1787172, Length=621, Percent_Identity=21.5780998389694, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000015
- InterPro:   IPR018030 [H]

Pfam domain/function: PF00577 Usher [H]

EC number: NA

Molecular weight: Translated: 88629; Mature: 88497

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01151 FIMBRIAL_USHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPGFGASGFRRNQSIDGRNTLMSPGWARRIQRPLWLMTGAWCLMFIHPSGAGELPPPP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECEEEEEEEECCCCCCCCCCC
SGMEAVSDAQLFLELVVNQMNTGRVVAVEQRGGRLFLPASVLRDTGMKLPEEAGAEVDLD
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECHHHHHHCCCCCCHHCCCEEECC
GLPGLHSDYDSVGQRLLLDVPPDWLPEQFIGNREVYPRTPALSSFGALFNYDLYLNDTDD
CCCCCCCCHHHHCCEEEEECCCCCCCHHHCCCCCCCCCCCCHHHCCCEEEEEEEEECCCC
AGTYLAAWNEVRVFDSWGTLSNTGQYRRTLSGDAVSTLDNGYLRYDTTWRYSDDERMLTY
CCCEEEECCEEEEEECCCCCCCCCHHHEECCCCHHHHCCCCEEEEEEEEEECCCCEEEEE
EAGDVVSGALPWSSSVRLGGVQFSRDFAVRPDLVTYPLPQFAGEAAVPSSVDLFINGYKS
ECCCEEECCCCCCCCEEECCEEECCCCCCCCCEEECCCHHHCCCCCCCCCEEEEEECCCC
SSTDLQPGPYTLTNIPFINGAGEAVVVTTDALGRQVSTTVPFYVTSSLLQKGLSDFSVAA
CCCCCCCCCEEEECCCEECCCCCEEEEEEHHHCCEECCCCCHHHHHHHHHCCCCHHHHHH
GTLRRDYGLKDFSYGPGVTSGSLRYGVSDTFTLESHAEAADTLTLGGLGGNLRLGNFGVL
HHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCCEEEEECCCCCEEECCCHHH
NSAISQSRFDGDGGQQLSLGYQYSGQRYSFSYQRLQRRDRYADLTVVDSPYTTLSKRSEQ
HHHHHHCCCCCCCCCEEEEEEEECCCEEEHHHHHHHHHCCCCCEEEECCCCHHHHCCCCC
ATLSLNLERWGSLGVGYFDIRAADETRTRLLNLSWSKPLWRNSSFYLSANREIGDSNWAV
EEEEEEHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEE
QAQLVIPFDLRGSLAISSERSKTGQTQQRVNYSRAVPSEGGVGFNLGYAKGDGADYRQAD
EEEEEEEEECCCCEEECCCCCCCCCHHHHCCHHHCCCCCCCCEEEECCCCCCCCCCEECC
VTWRLQSVQLQAGVYGTSDAETRWADASGSLVWMDDQVFAANRIDDAFVVVSTDGYADIP
EEEEEEEEEEEECCCCCCCCCCEECCCCCCEEEECCCEEECCCCCCEEEEEECCCCEECC
VRYENQQVGQTDRNGHLLVPWSSAYYRGKYEIDPLNLPANVRSPNVEQRIAVRRGSGYLL
EEECCCCCCCCCCCCEEEEECCCCEECCEEEECCCCCCCCCCCCCHHHEEEEECCCCEEE
EFPLSRVIAASIVLVDAQQKELPLGSGVLHEQSGTRTVVGWDGLVYLENLQAQNSLRVTL
ECCHHHHHEEEEEEEECCHHCCCCCCCEEECCCCCEEEEECCCEEEEECCCCCCCEEEEE
ADGKTCQAQFSVDLQQDEVPLIGPLVCQ
ECCCEEEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SKPGFGASGFRRNQSIDGRNTLMSPGWARRIQRPLWLMTGAWCLMFIHPSGAGELPPPP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECEEEEEEEECCCCCCCCCCC
SGMEAVSDAQLFLELVVNQMNTGRVVAVEQRGGRLFLPASVLRDTGMKLPEEAGAEVDLD
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECHHHHHHCCCCCCHHCCCEEECC
GLPGLHSDYDSVGQRLLLDVPPDWLPEQFIGNREVYPRTPALSSFGALFNYDLYLNDTDD
CCCCCCCCHHHHCCEEEEECCCCCCCHHHCCCCCCCCCCCCHHHCCCEEEEEEEEECCCC
AGTYLAAWNEVRVFDSWGTLSNTGQYRRTLSGDAVSTLDNGYLRYDTTWRYSDDERMLTY
CCCEEEECCEEEEEECCCCCCCCCHHHEECCCCHHHHCCCCEEEEEEEEEECCCCEEEEE
EAGDVVSGALPWSSSVRLGGVQFSRDFAVRPDLVTYPLPQFAGEAAVPSSVDLFINGYKS
ECCCEEECCCCCCCCEEECCEEECCCCCCCCCEEECCCHHHCCCCCCCCCEEEEEECCCC
SSTDLQPGPYTLTNIPFINGAGEAVVVTTDALGRQVSTTVPFYVTSSLLQKGLSDFSVAA
CCCCCCCCCEEEECCCEECCCCCEEEEEEHHHCCEECCCCCHHHHHHHHHCCCCHHHHHH
GTLRRDYGLKDFSYGPGVTSGSLRYGVSDTFTLESHAEAADTLTLGGLGGNLRLGNFGVL
HHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCCEEEEECCCCCEEECCCHHH
NSAISQSRFDGDGGQQLSLGYQYSGQRYSFSYQRLQRRDRYADLTVVDSPYTTLSKRSEQ
HHHHHHCCCCCCCCCEEEEEEEECCCEEEHHHHHHHHHCCCCCEEEECCCCHHHHCCCCC
ATLSLNLERWGSLGVGYFDIRAADETRTRLLNLSWSKPLWRNSSFYLSANREIGDSNWAV
EEEEEEHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEE
QAQLVIPFDLRGSLAISSERSKTGQTQQRVNYSRAVPSEGGVGFNLGYAKGDGADYRQAD
EEEEEEEEECCCCEEECCCCCCCCCHHHHCCHHHCCCCCCCCEEEECCCCCCCCCCEECC
VTWRLQSVQLQAGVYGTSDAETRWADASGSLVWMDDQVFAANRIDDAFVVVSTDGYADIP
EEEEEEEEEEEECCCCCCCCCCEECCCCCCEEEECCCEEECCCCCCEEEEEECCCCEECC
VRYENQQVGQTDRNGHLLVPWSSAYYRGKYEIDPLNLPANVRSPNVEQRIAVRRGSGYLL
EEECCCCCCCCCCCCEEEEECCCCEECCEEEECCCCCCCCCCCCCHHHEEEEECCCCEEE
EFPLSRVIAASIVLVDAQQKELPLGSGVLHEQSGTRTVVGWDGLVYLENLQAQNSLRVTL
ECCHHHHHEEEEEEEECCHHCCCCCCCEEECCCCCEEEEECCCEEEEECCCCCCCEEEEE
ADGKTCQAQFSVDLQQDEVPLIGPLVCQ
ECCCEEEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002584 [H]