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The map label for this gene is nuoB [H]

Identifier: 77459826

GI number: 77459826

Start: 4073392

End: 4074066

Strand: Direct

Name: nuoB [H]

Synonym: Pfl01_3604

Alternate gene names: 77459826

Gene position: 4073392-4074066 (Clockwise)

Preceding gene: 77459825

Following gene: 77459827

Centisome position: 63.27

GC content: 57.93

Gene sequence:

>675_bases
ATGCAATACAATCTCACCAGAATCGACCCCGATGCTCCTAACGAGCAGTATCCGATCGGCCAGCGGGAAACCGTTTCCGA
TCCGTTAGAAGATCAAGTCCACAAAAACATTTTCATGGGCAAGCTGGAAGACGTGCTGAGTGGCGCGGTCAACTGGGGAC
GTAAGAACTCCCTGTGGCCGTACAACTTCGGTCTGTCGTGCTGCTACGTGGAAATGACCACCGCCTTCACGGCGCCCCAC
GACATCGCGCGCTTCGGCGCCGAAGTTATCCGGGCTTCACCGCGTCAGGCTGATTTCATGGTTATCGCCGGGACCTGCTT
CATCAAGATGGCGCCGATCATTCAGCGCCTGTACGAGCAGATGCTCGAGCCGAAATGGGTTATCTCCATGGGTTCGTGCG
CCAACTCCGGCGGCATGTACGACATCTACTCCGTGGTTCAGGGGGTGGACAAGTTCCTGCCCGTTGACGTCTACGTACCT
GGCTGCCCGCCCCGTCCAGAAGCATTTCTGCAAGGTTTGATGCTGTTGCAGGAATCCATTGGCCAGGAGCGTCGCCCACT
GTCCTGGGTCGTTGGCGATCAAGGCGTTTATCGCGCCGACATGCCTTCGCAGAAGGAACAGCGCCGCGAACAGCGAATCG
CAGTCACCAACCTGCGCAGCCCTGACGAAGTCTGA

Upstream 100 bases:

>100_bases
TTGGCAGGTCTTGTCTACCTGTTCCGAGTGGGCGCCCTTGACTGGGCTCCGGAAGCTCGTCGCAAGCGGCAGGCGAAGCT
GAAACAATGAGGCTTTGGCA

Downstream 100 bases:

>100_bases
TCCAGTCTGTTTCAAAGAAACGAGAAGCTGGCTTCATTCTTTACGTTGACCGAAAGCGAAAAAATAACCATGACTACAGG
CAGTGCTCTGTACATCCCGC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQYNLTRIDPDAPNEQYPIGQRETVSDPLEDQVHKNIFMGKLEDVLSGAVNWGRKNSLWPYNFGLSCCYVEMTTAFTAPH
DIARFGAEVIRASPRQADFMVIAGTCFIKMAPIIQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVP
GCPPRPEAFLQGLMLLQESIGQERRPLSWVVGDQGVYRADMPSQKEQRREQRIAVTNLRSPDEV

Sequences:

>Translated_224_residues
MQYNLTRIDPDAPNEQYPIGQRETVSDPLEDQVHKNIFMGKLEDVLSGAVNWGRKNSLWPYNFGLSCCYVEMTTAFTAPH
DIARFGAEVIRASPRQADFMVIAGTCFIKMAPIIQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVP
GCPPRPEAFLQGLMLLQESIGQERRPLSWVVGDQGVYRADMPSQKEQRREQRIAVTNLRSPDEV
>Mature_224_residues
MQYNLTRIDPDAPNEQYPIGQRETVSDPLEDQVHKNIFMGKLEDVLSGAVNWGRKNSLWPYNFGLSCCYVEMTTAFTAPH
DIARFGAEVIRASPRQADFMVIAGTCFIKMAPIIQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVP
GCPPRPEAFLQGLMLLQESIGQERRPLSWVVGDQGVYRADMPSQKEQRREQRIAVTNLRSPDEV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=183, Percent_Identity=46.448087431694, Blast_Score=169, Evalue=1e-42,
Organism=Escherichia coli, GI1788624, Length=224, Percent_Identity=77.2321428571429, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=40.9836065573771, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=39.344262295082, Blast_Score=87, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=180, Percent_Identity=48.3333333333333, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=152, Percent_Identity=51.3157894736842, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24642371, Length=152, Percent_Identity=51.3157894736842, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24651058, Length=151, Percent_Identity=52.9801324503311, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25340; Mature: 25340

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYNLTRIDPDAPNEQYPIGQRETVSDPLEDQVHKNIFMGKLEDVLSGAVNWGRKNSLWP
CCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YNFGLSCCYVEMTTAFTAPHDIARFGAEVIRASPRQADFMVIAGTCFIKMAPIIQRLYEQ
CCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
MLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVPGCPPRPEAFLQGLMLLQESI
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GQERRPLSWVVGDQGVYRADMPSQKEQRREQRIAVTNLRSPDEV
CCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQYNLTRIDPDAPNEQYPIGQRETVSDPLEDQVHKNIFMGKLEDVLSGAVNWGRKNSLWP
CCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YNFGLSCCYVEMTTAFTAPHDIARFGAEVIRASPRQADFMVIAGTCFIKMAPIIQRLYEQ
CCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
MLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVPGCPPRPEAFLQGLMLLQESI
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GQERRPLSWVVGDQGVYRADMPSQKEQRREQRIAVTNLRSPDEV
CCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA