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The map label for this gene is kefB [H]

Identifier: 77459735

GI number: 77459735

Start: 3991840

End: 3993648

Strand: Direct

Name: kefB [H]

Synonym: Pfl01_3513

Alternate gene names: 77459735

Gene position: 3991840-3993648 (Clockwise)

Preceding gene: 77459733

Following gene: 77459741

Centisome position: 62.0

GC content: 61.91

Gene sequence:

>1809_bases
ATGCCCCATGAAGGCAATCTGTTGCAAGCCGCCGTCGTCTTTCTCCTGGCCGCCGTGCTCACCGTTCCCCTGGCCAAGCG
TCTGCAACTGGGCGCGGTGCTCGGTTATCTGTTTGCCGGGGTGATCATCGGCCCGTCGGTACTGGGTCTGATCGGCAATC
CGCAAAGTGTCAGCCATATCTCGGAACTGGGCGTGGTGCTGCTGTTGTTCATCATCGGTCTTGAGCTGTCGCCGCGACGT
TTGTGGGTGATGCGCAAATCGGTGTTCGGCGTGGGTCTGGCGCAGGTGCTGCTGACCGGTTCGGTGATCGGTGTGCTGGC
GTTGTCGGTGTTCGGCCAGCCGCTGAACAGCGCGATTGTTCTGGGCCTGGGCCTGGCGCTGTCGTCCACCGCATTCGGCC
TGCAAAGCCTCGCCGAGCGCAAGGAACTGACCAGCCCCCACGGGCGGTTGGCGTTTGCGATTCTGCTGTTCCAGGACATC
GCCGCGATCCCGTTGATCGCCCTGGTGCCGATGCTCGCGGGGGTCGATCACCACACCAGCACCGCCGACGATATTCGTCA
CAGTTTGCAGGTGCTCGGCAGTATCGCCGTGGTGGTGATCGGCGGGCGATATCTGTTGCGTCCGGTGTTTCGCGTGGTGG
CGAAAACCGGTCTGCCGGAAGTCTCCACCGCCACCGCGTTGCTGGTGGTGATCGGTACAGCGTGGCTGATGGACATGGTC
GGTGTGTCGATGGCGCTGGGCGCGTTTCTCGCCGGGTTGCTGTTGGCGGATTCGGAATACCGCCATGAGCTGGAAGCGCA
GATCGAACCGTTCAAGGGTCTGCTTCTCGGGCTGTTTTTCATCAGCGTCGGCATGGGCGCCAACCTCAGTCTGCTGCTCA
GCGCGCCAATCACGGTGCTGGGGCTGACGCTGTTGCTGATCGCCCTGAAGTTGCCGTTGTTGTTTGTAGTCGGGCGTCTG
GCCGGTGGATTGAACAAGGTCAGCGCGATTCGCCTCGGCATCGTGCTGGCAGCGGGCGGTGAGTTTGCTTTCGTAGTGTT
CAAGATTGGCCGCGATCAAGGCCTGTTTGAACCACGTCTGTACGACCTGCTGGTGCTGACCATCACCCTGTCGATGGCCG
TCACGCCGCTGTTGCTGCTGGTCTGCGCACGGCTGGTCAGCCCGAAGGTGCAGCCGGTGGAAGTGCCGGAGAAATTCCGC
CAGATCGAGACCGAATCCCCACGGGTAGTGATCGCCGGCATGGGCCGTATGGGGCAGATCGTGGCGCGGATCCTGCGGGC
GCAGAACATCAAGTTCGTGGCGCTGGACACCTCGGTGGAAACCATCGAACTGTCGCGCAGTTTTGGTGGCGTACCGGTGT
TCTACGGTGACCCGATGCGTCCGGAAATCCTCAGTGCGGCCAAGGTCGGTGAAGCGGAGTACTTCGTGATTGCCACGGAT
GATCCGGAAACCAACATCAAGACCGCTGAGATCGTGCGCAAGCTCTATCCAGACATGAAGATCATCGCCCGGGCGCGTAA
CCGGCAGCATGTGCATCGGCTGGTGGATGTGGGGGCCGATCCGATCCGGGAGACCTATTACTCAAGCCTGGAAATGAGCC
GCCGCACCCTGGTTGGCCTTGGCTTGACCCAGGCCCAGGCCGATGCGCGGATCAAGCGCTTCAAGCACCATGACGAACAG
GTGCTGGAAGCTCAACACGCGGTGTACGACGACGCGGCCAAAGTGCTGCAGACCGCCCAGGAAGCGCGGGCGGAACTGGC
CCGGTTGTTCGAGTCGGACCAACTGGAAGAACAATCAGGCAAGTCCTGA

Upstream 100 bases:

>100_bases
AGAAGCACATCTGCTCAAGCAGGCTGTGCGGTAGATCTGCAATGATTTCAGCCTTTATCCTTGTCGCCCCCCGCCGTACC
GCGTTACTGGAGTTCCGCTC

Downstream 100 bases:

>100_bases
GCCCCGCCATCAATCCTTCCCGCGCAATGCGCGGGAAGGTTCTGATCAGGCCATCTCCAGCGCCCGCTCCTCCTTCAGGG
GCGCCTCTGCAAACCGGTCC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MPHEGNLLQAAVVFLLAAVLTVPLAKRLQLGAVLGYLFAGVIIGPSVLGLIGNPQSVSHISELGVVLLLFIIGLELSPRR
LWVMRKSVFGVGLAQVLLTGSVIGVLALSVFGQPLNSAIVLGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDI
AAIPLIALVPMLAGVDHHTSTADDIRHSLQVLGSIAVVVIGGRYLLRPVFRVVAKTGLPEVSTATALLVVIGTAWLMDMV
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANLSLLLSAPITVLGLTLLLIALKLPLLFVVGRL
AGGLNKVSAIRLGIVLAAGGEFAFVVFKIGRDQGLFEPRLYDLLVLTITLSMAVTPLLLLVCARLVSPKVQPVEVPEKFR
QIETESPRVVIAGMGRMGQIVARILRAQNIKFVALDTSVETIELSRSFGGVPVFYGDPMRPEILSAAKVGEAEYFVIATD
DPETNIKTAEIVRKLYPDMKIIARARNRQHVHRLVDVGADPIRETYYSSLEMSRRTLVGLGLTQAQADARIKRFKHHDEQ
VLEAQHAVYDDAAKVLQTAQEARAELARLFESDQLEEQSGKS

Sequences:

>Translated_602_residues
MPHEGNLLQAAVVFLLAAVLTVPLAKRLQLGAVLGYLFAGVIIGPSVLGLIGNPQSVSHISELGVVLLLFIIGLELSPRR
LWVMRKSVFGVGLAQVLLTGSVIGVLALSVFGQPLNSAIVLGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDI
AAIPLIALVPMLAGVDHHTSTADDIRHSLQVLGSIAVVVIGGRYLLRPVFRVVAKTGLPEVSTATALLVVIGTAWLMDMV
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANLSLLLSAPITVLGLTLLLIALKLPLLFVVGRL
AGGLNKVSAIRLGIVLAAGGEFAFVVFKIGRDQGLFEPRLYDLLVLTITLSMAVTPLLLLVCARLVSPKVQPVEVPEKFR
QIETESPRVVIAGMGRMGQIVARILRAQNIKFVALDTSVETIELSRSFGGVPVFYGDPMRPEILSAAKVGEAEYFVIATD
DPETNIKTAEIVRKLYPDMKIIARARNRQHVHRLVDVGADPIRETYYSSLEMSRRTLVGLGLTQAQADARIKRFKHHDEQ
VLEAQHAVYDDAAKVLQTAQEARAELARLFESDQLEEQSGKS
>Mature_601_residues
PHEGNLLQAAVVFLLAAVLTVPLAKRLQLGAVLGYLFAGVIIGPSVLGLIGNPQSVSHISELGVVLLLFIIGLELSPRRL
WVMRKSVFGVGLAQVLLTGSVIGVLALSVFGQPLNSAIVLGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDIA
AIPLIALVPMLAGVDHHTSTADDIRHSLQVLGSIAVVVIGGRYLLRPVFRVVAKTGLPEVSTATALLVVIGTAWLMDMVG
VSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANLSLLLSAPITVLGLTLLLIALKLPLLFVVGRLA
GGLNKVSAIRLGIVLAAGGEFAFVVFKIGRDQGLFEPRLYDLLVLTITLSMAVTPLLLLVCARLVSPKVQPVEVPEKFRQ
IETESPRVVIAGMGRMGQIVARILRAQNIKFVALDTSVETIELSRSFGGVPVFYGDPMRPEILSAAKVGEAEYFVIATDD
PETNIKTAEIVRKLYPDMKIIARARNRQHVHRLVDVGADPIRETYYSSLEMSRRTLVGLGLTQAQADARIKRFKHHDEQV
LEAQHAVYDDAAKVLQTAQEARAELARLFESDQLEEQSGKS

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=400, Percent_Identity=27.5, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI1786232, Length=592, Percent_Identity=42.9054054054054, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1789749, Length=595, Percent_Identity=43.6974789915966, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786685, Length=553, Percent_Identity=28.75226039783, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322367, Length=406, Percent_Identity=23.3990147783251, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 64775; Mature: 64643

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHEGNLLQAAVVFLLAAVLTVPLAKRLQLGAVLGYLFAGVIIGPSVLGLIGNPQSVSHI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SELGVVLLLFIIGLELSPRRLWVMRKSVFGVGLAQVLLTGSVIGVLALSVFGQPLNSAIV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDIAAIPLIALVPMLAGVDHHTS
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TADDIRHSLQVLGSIAVVVIGGRYLLRPVFRVVAKTGLPEVSTATALLVVIGTAWLMDMV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANLSLLLSAPITVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHH
GLTLLLIALKLPLLFVVGRLAGGLNKVSAIRLGIVLAAGGEFAFVVFKIGRDQGLFEPRL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCEEEEEEEECCCCCCCCHHH
YDLLVLTITLSMAVTPLLLLVCARLVSPKVQPVEVPEKFRQIETESPRVVIAGMGRMGQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEECCCHHHHH
VARILRAQNIKFVALDTSVETIELSRSFGGVPVFYGDPMRPEILSAAKVGEAEYFVIATD
HHHHHHHCCCEEEEECCCCHHHEEHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECC
DPETNIKTAEIVRKLYPDMKIIARARNRQHVHRLVDVGADPIRETYYSSLEMSRRTLVGL
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GLTQAQADARIKRFKHHDEQVLEAQHAVYDDAAKVLQTAQEARAELARLFESDQLEEQSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KS
CC
>Mature Secondary Structure 
PHEGNLLQAAVVFLLAAVLTVPLAKRLQLGAVLGYLFAGVIIGPSVLGLIGNPQSVSHI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SELGVVLLLFIIGLELSPRRLWVMRKSVFGVGLAQVLLTGSVIGVLALSVFGQPLNSAIV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDIAAIPLIALVPMLAGVDHHTS
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TADDIRHSLQVLGSIAVVVIGGRYLLRPVFRVVAKTGLPEVSTATALLVVIGTAWLMDMV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANLSLLLSAPITVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHH
GLTLLLIALKLPLLFVVGRLAGGLNKVSAIRLGIVLAAGGEFAFVVFKIGRDQGLFEPRL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCEEEEEEEECCCCCCCCHHH
YDLLVLTITLSMAVTPLLLLVCARLVSPKVQPVEVPEKFRQIETESPRVVIAGMGRMGQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEECCCHHHHH
VARILRAQNIKFVALDTSVETIELSRSFGGVPVFYGDPMRPEILSAAKVGEAEYFVIATD
HHHHHHHCCCEEEEECCCCHHHEEHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECC
DPETNIKTAEIVRKLYPDMKIIARARNRQHVHRLVDVGADPIRETYYSSLEMSRRTLVGL
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GLTQAQADARIKRFKHHDEQVLEAQHAVYDDAAKVLQTAQEARAELARLFESDQLEEQSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA