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The map label for this gene is cheR [C]

Identifier: 77458872

GI number: 77458872

Start: 3034876

End: 3035709

Strand: Direct

Name: cheR [C]

Synonym: Pfl01_2647

Alternate gene names: 77458872

Gene position: 3034876-3035709 (Clockwise)

Preceding gene: 77458871

Following gene: 77458873

Centisome position: 47.14

GC content: 54.44

Gene sequence:

>834_bases
GTGGAACGTAGTAATCCCGTTGAACGAAACAGTGAAATCGAACTGCGGCTGTTGATTGAGGCAATTTATCTCAAGTACAG
CTATGACTTTCGCGATTACTCCGGCGCCTCGATCAAGCGCCGGGTGCAACATGCGCTGAGCCAGTTCGAGTGCGCGACCA
TCTCGGCCCTGCAGGAGAAAGTCCTGCATGACCCGACGGCGTTCATGCAGTTGCTGCAATTGCTGACGATCCCGGTCAGC
GAGATGTTCCGCGATCCGTCGCACTTCCTGGCGATCCGTCAGGAGGTGGTGCCGCTGCTCAAGACCTATCCGTCGATCAA
GATCTGGATCGCCGGTTGCAGCACCGGCGAGGAGGTCTACTCGATGGCGATTCTGCTGCGCGAGGAAGGCCTGCTCGACC
GCACGATCATCTACGCCACCGACATCAACCCGCGCTCGCTGGAAAAGGCCAAGCAAGGGATTTTTTCGATGGAAAATGTG
CGTGCGTACACCGCCAATTATCAGCAGGCGGGCGGCCAGCGCTCGTTCGCCGACTACTACACTGCTGCCTATGGCTACGC
GATTTTCGACAAGAGCCTGTGCGAAAACGTGACCTTCGCCGATCACAGCCTGGCGACCGATAGTGTATTCTCCGAAACTC
AATTAATTTCGTGTCGTAACGTGTTGATTTACTTCAATAAAAAACTTCAGGATCGAGCGTTCGGGTTGTTCCATGAATCG
TTGTGCCACCGTGGATTCCTGGTATTGGGCAGCAAGGAAACTTTGGATTTTTCCGGCTACTCCAACCAGTTCGAGCCGCT
GGTGAAACAAGAACGGATCTACCGCAAATCATGA

Upstream 100 bases:

>100_bases
GCCTGCAGGCGGGCGCCAATGATTACCTGGCCAAGCCCATTGACCTGGATCGCCTGTTCTCGCTGATTCGCGTGTGGTTG
CCGAAGATGGAACGCATTTA

Downstream 100 bases:

>100_bases
ACAGTGCAGCGGATTTGCCTCGGGTCGAGGCGATTGTGGTCGGAGCTTCCGCCGGTGGCGTCGAGGCTTTGTTGACGATG
CTCGGTTCCTTGAGGGCCGG

Product: MCP methyltransferase, CheR-type

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MERSNPVERNSEIELRLLIEAIYLKYSYDFRDYSGASIKRRVQHALSQFECATISALQEKVLHDPTAFMQLLQLLTIPVS
EMFRDPSHFLAIRQEVVPLLKTYPSIKIWIAGCSTGEEVYSMAILLREEGLLDRTIIYATDINPRSLEKAKQGIFSMENV
RAYTANYQQAGGQRSFADYYTAAYGYAIFDKSLCENVTFADHSLATDSVFSETQLISCRNVLIYFNKKLQDRAFGLFHES
LCHRGFLVLGSKETLDFSGYSNQFEPLVKQERIYRKS

Sequences:

>Translated_277_residues
MERSNPVERNSEIELRLLIEAIYLKYSYDFRDYSGASIKRRVQHALSQFECATISALQEKVLHDPTAFMQLLQLLTIPVS
EMFRDPSHFLAIRQEVVPLLKTYPSIKIWIAGCSTGEEVYSMAILLREEGLLDRTIIYATDINPRSLEKAKQGIFSMENV
RAYTANYQQAGGQRSFADYYTAAYGYAIFDKSLCENVTFADHSLATDSVFSETQLISCRNVLIYFNKKLQDRAFGLFHES
LCHRGFLVLGSKETLDFSGYSNQFEPLVKQERIYRKS
>Mature_277_residues
MERSNPVERNSEIELRLLIEAIYLKYSYDFRDYSGASIKRRVQHALSQFECATISALQEKVLHDPTAFMQLLQLLTIPVS
EMFRDPSHFLAIRQEVVPLLKTYPSIKIWIAGCSTGEEVYSMAILLREEGLLDRTIIYATDINPRSLEKAKQGIFSMENV
RAYTANYQQAGGQRSFADYYTAAYGYAIFDKSLCENVTFADHSLATDSVFSETQLISCRNVLIYFNKKLQDRAFGLFHES
LCHRGFLVLGSKETLDFSGYSNQFEPLVKQERIYRKS

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=205, Percent_Identity=30.2439024390244, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80

Molecular weight: Translated: 31835; Mature: 31835

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERSNPVERNSEIELRLLIEAIYLKYSYDFRDYSGASIKRRVQHALSQFECATISALQEK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLHDPTAFMQLLQLLTIPVSEMFRDPSHFLAIRQEVVPLLKTYPSIKIWIAGCSTGEEVY
HHCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
SMAILLREEGLLDRTIIYATDINPRSLEKAKQGIFSMENVRAYTANYQQAGGQRSFADYY
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHH
TAAYGYAIFDKSLCENVTFADHSLATDSVFSETQLISCRNVLIYFNKKLQDRAFGLFHES
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHH
LCHRGFLVLGSKETLDFSGYSNQFEPLVKQERIYRKS
HHHCCEEEECCCCCEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERSNPVERNSEIELRLLIEAIYLKYSYDFRDYSGASIKRRVQHALSQFECATISALQEK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLHDPTAFMQLLQLLTIPVSEMFRDPSHFLAIRQEVVPLLKTYPSIKIWIAGCSTGEEVY
HHCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
SMAILLREEGLLDRTIIYATDINPRSLEKAKQGIFSMENVRAYTANYQQAGGQRSFADYY
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHH
TAAYGYAIFDKSLCENVTFADHSLATDSVFSETQLISCRNVLIYFNKKLQDRAFGLFHES
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHH
LCHRGFLVLGSKETLDFSGYSNQFEPLVKQERIYRKS
HHHCCEEEECCCCCEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-adenosyl-L-methionine; protein L-glutamate

Specific reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]