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The map label for this gene is cysK [H]

Identifier: 77457640

GI number: 77457640

Start: 1583201

End: 1584175

Strand: Direct

Name: cysK [H]

Synonym: Pfl01_1413

Alternate gene names: 77457640

Gene position: 1583201-1584175 (Clockwise)

Preceding gene: 77457638

Following gene: 77457641

Centisome position: 24.59

GC content: 60.62

Gene sequence:

>975_bases
ATGAGCCGTATTTTTGCTGACAACGCCCATTCCATCGGCAACACGCCGCTGGTGCAGATCAACCGCATTGCGCCCCGTGG
CGTGACCATTCTGGCCAAGATCGAGGGTCGCAACCCGGGCTACTCGGTCAAGTGCCGGATTGGCGCCAACATGATCTGGG
ACGCCGAAAGCAGCGGCAAACTCAAGCCGGGCATGACCATCGTCGAGCCGACCTCCGGCAACACCGGCATCGGTCTGGCC
TTCGTGGCGGCCGCTCGCGGTTACAAATTGCTGCTGACCATGCCGGCCTCGATGAGCATCGAGCGGCGCAAGGTGCTCAA
GGCGCTGGGTGCCGAGCTGGTGCTGACCGAACCAGCCAAAGGCATGAAAGGCGCTATCGAAAAGGCCAACGAAATCGTAG
CCAGTGACGCGGGCAAATACTTCATGCCGGCCCAGTTCGATAATCCGGCCAACCCAGCCATTCATGAAAAGACCACCGGC
CCGGAAATCTGGAATGACACCGATGGCGCCATCGATGTGCTGGTGGCGGGCGTCGGGACCGGCGGAACCATCACCGGCGT
GTCGCGGTATATCAAGAATACGGCAGGCAAACCGATTCTGTCGGTGGCGGTGGAACCGGTGTCTTCGCCGGTGATTACCC
AGGCGCTGGCAGGTCAAGAAATCAAGCCGAGCCCGCACAAGATTCAGGGCATCGGTGCCGGGTTCGTGCCGAAGAACCTT
GATCTGTCGATGGTTGACCGGGTCGAGTTGGTGACGGATGACGAGTCCAAAGCCATGGCCCTGCGCCTGATGCAGGAGGA
AGGGATCCTGTGCGGTATTTCCTGCGGTGCGGCCATGTCGGTGGCGGTGCGTCTGGCGGAGACGCCGGAAATGCAGGGCA
AGACCATTGTGGTGGTGCTGCCCGACTCCGGTGAGCGTTATCTGTCGAGCATGCTGTTCAGTGATCTGTTCACCGATCAG
GAGAACCAGCAGTAA

Upstream 100 bases:

>100_bases
GTTGACTGCCGCCTGCCGCAGACGTTCGACCGAACTGGACTGAAAAAGGCAACAGGCTTACCTTGGAGAGCTGAATTCAA
CGCTGCACAAGGACCCAGCC

Downstream 100 bases:

>100_bases
GTGGCGTGTTGATTCAGGTCAGCCAGGGTTCAGGATCGTTATGTTAATAAGTGCTTTGTTGCGCAATTCTTAACACTGAA
TCTTGAGTCGGGCGGGTTTT

Product: cysteine synthase

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGKLKPGMTIVEPTSGNTGIGLA
FVAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAKGMKGAIEKANEIVASDAGKYFMPAQFDNPANPAIHEKTTG
PEIWNDTDGAIDVLVAGVGTGGTITGVSRYIKNTAGKPILSVAVEPVSSPVITQALAGQEIKPSPHKIQGIGAGFVPKNL
DLSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMSVAVRLAETPEMQGKTIVVVLPDSGERYLSSMLFSDLFTDQ
ENQQ

Sequences:

>Translated_324_residues
MSRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGKLKPGMTIVEPTSGNTGIGLA
FVAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAKGMKGAIEKANEIVASDAGKYFMPAQFDNPANPAIHEKTTG
PEIWNDTDGAIDVLVAGVGTGGTITGVSRYIKNTAGKPILSVAVEPVSSPVITQALAGQEIKPSPHKIQGIGAGFVPKNL
DLSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMSVAVRLAETPEMQGKTIVVVLPDSGERYLSSMLFSDLFTDQ
ENQQ
>Mature_323_residues
SRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGKLKPGMTIVEPTSGNTGIGLAF
VAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAKGMKGAIEKANEIVASDAGKYFMPAQFDNPANPAIHEKTTGP
EIWNDTDGAIDVLVAGVGTGGTITGVSRYIKNTAGKPILSVAVEPVSSPVITQALAGQEIKPSPHKIQGIGAGFVPKNLD
LSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMSVAVRLAETPEMQGKTIVVVLPDSGERYLSSMLFSDLFTDQE
NQQ

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=331, Percent_Identity=41.9939577039275, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI295821200, Length=331, Percent_Identity=41.9939577039275, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI4557415, Length=331, Percent_Identity=41.9939577039275, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1788754, Length=325, Percent_Identity=69.5384615384615, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI2367138, Length=313, Percent_Identity=39.9361022364217, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17535051, Length=316, Percent_Identity=49.0506329113924, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI115535073, Length=309, Percent_Identity=54.3689320388349, Blast_Score=277, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17562970, Length=325, Percent_Identity=45.2307692307692, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI32566674, Length=314, Percent_Identity=46.1783439490446, Blast_Score=250, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17561720, Length=325, Percent_Identity=46.1538461538462, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI32566672, Length=227, Percent_Identity=44.9339207048458, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=39.3188854489164, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=37.3088685015291, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17561716, Length=116, Percent_Identity=43.1034482758621, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321594, Length=327, Percent_Identity=37.6146788990826, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=34.8765432098765, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24643623, Length=317, Percent_Identity=37.8548895899054, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20129101, Length=317, Percent_Identity=37.8548895899054, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34261; Mature: 34130

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGK
CCCEECCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCEEEEECCCCCC
LKPGMTIVEPTSGNTGIGLAFVAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAK
CCCCCEEEECCCCCCCCHHHEEHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCC
GMKGAIEKANEIVASDAGKYFMPAQFDNPANPAIHEKTTGPEIWNDTDGAIDVLVAGVGT
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
GGTITGVSRYIKNTAGKPILSVAVEPVSSPVITQALAGQEIKPSPHKIQGIGAGFVPKNL
CCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
DLSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMSVAVRLAETPEMQGKTIVVVL
CHHHHCEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHEEECCCCCCCCCEEEEEE
PDSGERYLSSMLFSDLFTDQENQQ
CCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGK
CCEECCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCEEEEECCCCCC
LKPGMTIVEPTSGNTGIGLAFVAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAK
CCCCCEEEECCCCCCCCHHHEEHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCC
GMKGAIEKANEIVASDAGKYFMPAQFDNPANPAIHEKTTGPEIWNDTDGAIDVLVAGVGT
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
GGTITGVSRYIKNTAGKPILSVAVEPVSSPVITQALAGQEIKPSPHKIQGIGAGFVPKNL
CCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
DLSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMSVAVRLAETPEMQGKTIVVVL
CHHHHCEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHEEECCCCCCCCCEEEEEE
PDSGERYLSSMLFSDLFTDQENQQ
CCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]