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The map label for this gene is yafJ [H]

Identifier: 77457628

GI number: 77457628

Start: 1572870

End: 1573646

Strand: Direct

Name: yafJ [H]

Synonym: Pfl01_1401

Alternate gene names: 77457628

Gene position: 1572870-1573646 (Clockwise)

Preceding gene: 77457627

Following gene: 77457629

Centisome position: 24.43

GC content: 61.65

Gene sequence:

>777_bases
ATGTGTGAATTACTGGGCATGAGTGCCAACGTGCCGACCGACATCGTGTTCAGCTTCACCGGGTTGATGCAGCGCGGTGG
TCGCACCGGGCCACACCGCGACGGCTGGGGCATTGCCTTCTACGAAGGGCGCGGCTTGCGCCTGTTCCAGGACCCGGCGG
CCAGCAGCGAGTCGGAAGTCGCCAATCTGGTGCAGCGTTATCCGATCAAGAGCGAAGTGGTCATCGGTCACATCCGCCAG
GCCAACGTCGGCAAGGTCTGCCTGTCCAATACCCACCCGTTCGTCCGCGAGCTGTGGGGGCGCAACTGGTGCTTCGCGCA
CAATGGCCAGCTCGCCGATTTCTCCCCGATCAAAAGTTTCTACCGCCCGGTCGGCGATACCGACAGCGAAGCGGCGTTCT
GCGATTTGCTCAACCGGGTACGTGCAGCGTTTCCGGAACCGGTCGATATAGAAGTGCTGCTGCCGGATCTGGTCGCAGCC
TGCGCCGAATATCGCAGCAAAGGCGTGTTCAACTGCCTGCTCAGCGACGGCGACTGGCTGTTCTGCTATTGCTCGACCAA
ACTGGCGCAGATCACCCGTCGTGCACCGTTTGGCCCGGCGCGGCTCAAGGACGTCGATGTGATCGTCGATTTCCAGGCCG
AAACCACGCCCAACGACGTGGTGACGGTGATTGCCACTGAACCCTTGACCGAAAACGAAACCTGGACCCGCTACGAACCG
GGCCAATGGAGCCTGTGGCGACGCGGCGAATGCGTAAGCCAAGGCACGACCGAATAA

Upstream 100 bases:

>100_bases
TTCGGCGGCGCCGGCGATGACCGCTACGGTCGCCCGCCGGTACCGAGCAATCCGCAGCGCATGCGGGTCGAGCCGCGCCT
GTAATCACGAGAGTCCGCAC

Downstream 100 bases:

>100_bases
GGATTGCACCCCCATGTTGCTCAGTTATCTACGGCTGGTGTTGTTTGCGGCGGGCCTGTTGATCGGTGTCCAGGTGCCGG
GCTTCATCAACGATTACGCC

Product: glutamine amidotransferase, class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEVANLVQRYPIKSEVVIGHIRQ
ANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFSPIKSFYRPVGDTDSEAAFCDLLNRVRAAFPEPVDIEVLLPDLVAA
CAEYRSKGVFNCLLSDGDWLFCYCSTKLAQITRRAPFGPARLKDVDVIVDFQAETTPNDVVTVIATEPLTENETWTRYEP
GQWSLWRRGECVSQGTTE

Sequences:

>Translated_258_residues
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEVANLVQRYPIKSEVVIGHIRQ
ANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFSPIKSFYRPVGDTDSEAAFCDLLNRVRAAFPEPVDIEVLLPDLVAA
CAEYRSKGVFNCLLSDGDWLFCYCSTKLAQITRRAPFGPARLKDVDVIVDFQAETTPNDVVTVIATEPLTENETWTRYEP
GQWSLWRRGECVSQGTTE
>Mature_258_residues
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEVANLVQRYPIKSEVVIGHIRQ
ANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFSPIKSFYRPVGDTDSEAAFCDLLNRVRAAFPEPVDIEVLLPDLVAA
CAEYRSKGVFNCLLSDGDWLFCYCSTKLAQITRRAPFGPARLKDVDVIVDFQAETTPNDVVTVIATEPLTENETWTRYEP
GQWSLWRRGECVSQGTTE

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=61.4173228346457, Blast_Score=318, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28816; Mature: 28816

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEV
CCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHH
ANLVQRYPIKSEVVIGHIRQANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFSPIKSF
HHHHHHCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHH
YRPVGDTDSEAAFCDLLNRVRAAFPEPVDIEVLLPDLVAACAEYRSKGVFNCLLSDGDWL
HCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCEEEEEECCCCEE
FCYCSTKLAQITRRAPFGPARLKDVDVIVDFQAETTPNDVVTVIATEPLTENETWTRYEP
EEEECCHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCEECC
GQWSLWRRGECVSQGTTE
CCCCCEECCCCCCCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEV
CCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHH
ANLVQRYPIKSEVVIGHIRQANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFSPIKSF
HHHHHHCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHH
YRPVGDTDSEAAFCDLLNRVRAAFPEPVDIEVLLPDLVAACAEYRSKGVFNCLLSDGDWL
HCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCEEEEEECCCCEE
FCYCSTKLAQITRRAPFGPARLKDVDVIVDFQAETTPNDVVTVIATEPLTENETWTRYEP
EEEECCHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCEECC
GQWSLWRRGECVSQGTTE
CCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]