Click here to switch to the map view.

The map label for this gene is purT [H]

Identifier: 77457241

GI number: 77457241

Start: 1177764

End: 1178945

Strand: Direct

Name: purT [H]

Synonym: Pfl01_1014

Alternate gene names: 77457241

Gene position: 1177764-1178945 (Clockwise)

Preceding gene: 77457240

Following gene: 77457245

Centisome position: 18.29

GC content: 62.44

Gene sequence:

>1182_bases
ATGACCCGTATCGGAACTCCATTGTCGCCGACCGCGACCCGCGTATTGCTGTGTGGCTGTGGTGAGTTGGGCAAGGAAGT
GGTGATCGAGCTGCAACGCCTGGGCGTTGAAGTGATCGCCGTTGACCGTTACGCCAACGCGCCGGCCATGCAGGTTGCCC
ATCGCAGCCACGTGATCAACATGCTCGATGGTGCCGCCCTTCGTGCAGTGATCGAAGCCGAGAAGCCGCACTTCATCGTG
CCGGAAATCGAAGCCATCGCCACTGCCACGCTGGTCGCACTGGAAGCCGAAGGCTTCACTGTGATCCCGACCGCTCGCGC
CGCGCAACTGACCATGAACCGCGAAGGCATCCGTCGTCTGGCTGCCGAAGAGCTGGACCTGCCGACCTCGCCGTACCACT
TCGCCGACACCTTCGAGGACTACAGCAAGGCCGTTCAGGACCTCGGCTTTCCGTGCGTCGTGAAACCAGTGATGAGTTCG
TCGGGCAAGGGTCAGAGCCTGCTGCGCAGCGCCGATGACGTGCAGAAAGCCTGGGATTACGCCCAAGAGGGCGGTCGCGC
CGGCAAAGGTCGCGTGATCATCGAAGGTTTCATCGATTTCGACTACGAAATCACTCTGCTGACCGTGCGTCACGTCGGCG
GCACGACGTTCTGTGCGCCGGTCGGCCACCGTCAGGAGAAGGGCGACTATCAGGAATCCTGGCAGCCACAGGCCATGAGC
CCGGTGGCGCTGGCTGAATCCGAGCGTGTAGCCAAAGCCGTGACCGAAGCGTTGGGCGGTCGTGGTCTGTTCGGCGTCGA
GCTGTTCATCAAGGGCGATCAGGTGTGGTTCAGTGAAGTGTCGCCACGTCCACACGACACCGGTCTGGTAACCCTGATTT
CCCAGGACCTGTCGCAGTTCGCCCTGCACGCCCGAGCCATCCTCGGCCTGCCGGTGCCACTGATCCGTCAGTTCGGCCCA
TCGGCTTCGGCGGTGATTTTGGTGGAAGGCCAGTCGACCCAGACTGCATTCGCCAACCTCGGCGCTGCACTGAGCGAACC
GGATACCGCGCTGCGTCTGTTCGGCAAGCCTGAAGTCAATGGCCAGCGTCGCATGGGTGTTGCACTGGCCCGTGACGAGT
CGATCGAGGCCGCTCGTGCCAAGGCGACCCGTGCTTCTCAGGCTGTCGTTGTAGAGCTGTAA

Upstream 100 bases:

>100_bases
AGCCAAACCCTCAGGTTCATGTAAAATGCCGCGCTTTCTCCCCATGGAAAGCGCGTCCGGCGTTGCTTTCCTCGATGATC
CGCTTCTTTGAGGACCTGAG

Downstream 100 bases:

>100_bases
TCTCGCAACCTGATCGCTCCCGCATCGGGCGGGAGCGATCAGGGCATTTACGACACCTGATTCAAATCGTTATTGCGCGT
TTCCTTCAGGCACAGCACAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTRIGTPLSPTATRVLLCGCGELGKEVVIELQRLGVEVIAVDRYANAPAMQVAHRSHVINMLDGAALRAVIEAEKPHFIV
PEIEAIATATLVALEAEGFTVIPTARAAQLTMNREGIRRLAAEELDLPTSPYHFADTFEDYSKAVQDLGFPCVVKPVMSS
SGKGQSLLRSADDVQKAWDYAQEGGRAGKGRVIIEGFIDFDYEITLLTVRHVGGTTFCAPVGHRQEKGDYQESWQPQAMS
PVALAESERVAKAVTEALGGRGLFGVELFIKGDQVWFSEVSPRPHDTGLVTLISQDLSQFALHARAILGLPVPLIRQFGP
SASAVILVEGQSTQTAFANLGAALSEPDTALRLFGKPEVNGQRRMGVALARDESIEAARAKATRASQAVVVEL

Sequences:

>Translated_393_residues
MTRIGTPLSPTATRVLLCGCGELGKEVVIELQRLGVEVIAVDRYANAPAMQVAHRSHVINMLDGAALRAVIEAEKPHFIV
PEIEAIATATLVALEAEGFTVIPTARAAQLTMNREGIRRLAAEELDLPTSPYHFADTFEDYSKAVQDLGFPCVVKPVMSS
SGKGQSLLRSADDVQKAWDYAQEGGRAGKGRVIIEGFIDFDYEITLLTVRHVGGTTFCAPVGHRQEKGDYQESWQPQAMS
PVALAESERVAKAVTEALGGRGLFGVELFIKGDQVWFSEVSPRPHDTGLVTLISQDLSQFALHARAILGLPVPLIRQFGP
SASAVILVEGQSTQTAFANLGAALSEPDTALRLFGKPEVNGQRRMGVALARDESIEAARAKATRASQAVVVEL
>Mature_392_residues
TRIGTPLSPTATRVLLCGCGELGKEVVIELQRLGVEVIAVDRYANAPAMQVAHRSHVINMLDGAALRAVIEAEKPHFIVP
EIEAIATATLVALEAEGFTVIPTARAAQLTMNREGIRRLAAEELDLPTSPYHFADTFEDYSKAVQDLGFPCVVKPVMSSS
GKGQSLLRSADDVQKAWDYAQEGGRAGKGRVIIEGFIDFDYEITLLTVRHVGGTTFCAPVGHRQEKGDYQESWQPQAMSP
VALAESERVAKAVTEALGGRGLFGVELFIKGDQVWFSEVSPRPHDTGLVTLISQDLSQFALHARAILGLPVPLIRQFGPS
ASAVILVEGQSTQTAFANLGAALSEPDTALRLFGKPEVNGQRRMGVALARDESIEAARAKATRASQAVVVEL

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=67.3469387755102, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1786733, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=365, Percent_Identity=23.2876712328767, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42235; Mature: 42104

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIGTPLSPTATRVLLCGCGELGKEVVIELQRLGVEVIAVDRYANAPAMQVAHRSHVIN
CCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHH
MLDGAALRAVIEAEKPHFIVPEIEAIATATLVALEAEGFTVIPTARAAQLTMNREGIRRL
HHCHHHHHHHHHCCCCEEECCCHHHHHHEEEEEEECCCEEEECCCCHHHEEECHHHHHHH
AAEELDLPTSPYHFADTFEDYSKAVQDLGFPCVVKPVMSSSGKGQSLLRSADDVQKAWDY
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQEGGRAGKGRVIIEGFIDFDYEITLLTVRHVGGTTFCAPVGHRQEKGDYQESWQPQAMS
HHHCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCEEECCCCCCCCCCCHHHCCCCCCCC
PVALAESERVAKAVTEALGGRGLFGVELFIKGDQVWFSEVSPRPHDTGLVTLISQDLSQF
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHH
ALHARAILGLPVPLIRQFGPSASAVILVEGQSTQTAFANLGAALSEPDTALRLFGKPEVN
HHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCCC
GQRRMGVALARDESIEAARAKATRASQAVVVEL
CCHHCCEEEECCCCHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TRIGTPLSPTATRVLLCGCGELGKEVVIELQRLGVEVIAVDRYANAPAMQVAHRSHVIN
CCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHH
MLDGAALRAVIEAEKPHFIVPEIEAIATATLVALEAEGFTVIPTARAAQLTMNREGIRRL
HHCHHHHHHHHHCCCCEEECCCHHHHHHEEEEEEECCCEEEECCCCHHHEEECHHHHHHH
AAEELDLPTSPYHFADTFEDYSKAVQDLGFPCVVKPVMSSSGKGQSLLRSADDVQKAWDY
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQEGGRAGKGRVIIEGFIDFDYEITLLTVRHVGGTTFCAPVGHRQEKGDYQESWQPQAMS
HHHCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCEEECCCCCCCCCCCHHHCCCCCCCC
PVALAESERVAKAVTEALGGRGLFGVELFIKGDQVWFSEVSPRPHDTGLVTLISQDLSQF
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHH
ALHARAILGLPVPLIRQFGPSASAVILVEGQSTQTAFANLGAALSEPDTALRLFGKPEVN
HHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCCC
GQRRMGVALARDESIEAARAKATRASQAVVVEL
CCHHCCEEEECCCCHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA