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The map label for this gene is algF [H]

Identifier: 77457177

GI number: 77457177

Start: 1106142

End: 1106798

Strand: Reverse

Name: algF [H]

Synonym: Pfl01_0950

Alternate gene names: 77457177

Gene position: 1106798-1106142 (Counterclockwise)

Preceding gene: 77457178

Following gene: 77457176

Centisome position: 17.19

GC content: 63.62

Gene sequence:

>657_bases
ATGACTTTCACTACTACTCCTCGTCGTCTCGCCAAACGCTTCGCTCTGGTTGCGGGTCTGAGCGTGCTGTCGGTCCAGGC
CTTCGCCGGTGGCGACGCTGCGCTGTACGGCCCGACTGCACCGAAAGGCTCGACCTTCGTACGGGTCTACAACGCCAGCA
ACGCTGAAGTCAGCGCCACCGTCGGCAGCACCAATCTGAGCGACGTCGCGCCGCTGGCCAGCAGCGACTTCAGCTTCATG
CCGGGCGGTGACTACAGCGCCAAGGTCGGCAGCCAGACCCTGCCGGTCAAACTCGCCGGTGACCATTACTACACCCTGGT
CAACAACGCCTCCGGCGCGCCGCAACTGATCGAAGAACCGCCGTTCAAGAACAAGCAGAAATCCCTGGTGCGCGTACAGA
ACCTCAGCGACAAACCGCTGACCCTGAAGACCGCCGACGGCAAGACCGAGGTGGTGCCGAACGTCGCCGCCAAGGGCCGT
GGCGAGCGTGAAATCAACCCGGTGAAAGTCAGCCTGGCGCTGTACGAAGGCGACAAGAAAGTCGGCGACGTGAAACCGGT
CGCCCTGGAGCGCGGTGAGGCAGCGGTGCTGTACGTCACCGGCAACGGCAGCAACCTGTCGCCAGTCTGGGTGAAACGCC
CGGTGTCGACCCGCTAA

Upstream 100 bases:

>100_bases
CGCAGAGCTTAAACAAGCCGGTGCTCGCCAACAAAACGTAGCCATCAACACTAAATCCGAGACGCCCGATCGGGCGCAAA
ACTGAAAGAGAGGTAACACC

Downstream 100 bases:

>100_bases
GCACATGAAACCGGGCAGCCGTCGCTGCCCGGTACGGAACAACAACAAGAGTGAAACGACACAACGTTCGTGGCTCTGAC
CCAATCGATTTTATGGAGTA

Product: alginate biosynthesis protein AlgF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTFTTTPRRLAKRFALVAGLSVLSVQAFAGGDAALYGPTAPKGSTFVRVYNASNAEVSATVGSTNLSDVAPLASSDFSFM
PGGDYSAKVGSQTLPVKLAGDHYYTLVNNASGAPQLIEEPPFKNKQKSLVRVQNLSDKPLTLKTADGKTEVVPNVAAKGR
GEREINPVKVSLALYEGDKKVGDVKPVALERGEAAVLYVTGNGSNLSPVWVKRPVSTR

Sequences:

>Translated_218_residues
MTFTTTPRRLAKRFALVAGLSVLSVQAFAGGDAALYGPTAPKGSTFVRVYNASNAEVSATVGSTNLSDVAPLASSDFSFM
PGGDYSAKVGSQTLPVKLAGDHYYTLVNNASGAPQLIEEPPFKNKQKSLVRVQNLSDKPLTLKTADGKTEVVPNVAAKGR
GEREINPVKVSLALYEGDKKVGDVKPVALERGEAAVLYVTGNGSNLSPVWVKRPVSTR
>Mature_217_residues
TFTTTPRRLAKRFALVAGLSVLSVQAFAGGDAALYGPTAPKGSTFVRVYNASNAEVSATVGSTNLSDVAPLASSDFSFMP
GGDYSAKVGSQTLPVKLAGDHYYTLVNNASGAPQLIEEPPFKNKQKSLVRVQNLSDKPLTLKTADGKTEVVPNVAAKGRG
EREINPVKVSLALYEGDKKVGDVKPVALERGEAAVLYVTGNGSNLSPVWVKRPVSTR

Specific function: Together with AlgI and AlgJ, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlgF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22967; Mature: 22836

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTTTPRRLAKRFALVAGLSVLSVQAFAGGDAALYGPTAPKGSTFVRVYNASNAEVSAT
CCCCCCHHHHHHHHHHHHCCHHEEEEEECCCCEEEECCCCCCCCEEEEEEECCCCEEEEE
VGSTNLSDVAPLASSDFSFMPGGDYSAKVGSQTLPVKLAGDHYYTLVNNASGAPQLIEEP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHCCC
PFKNKQKSLVRVQNLSDKPLTLKTADGKTEVVPNVAAKGRGEREINPVKVSLALYEGDKK
CCCCCHHHHEEEECCCCCCEEEEECCCCCEECCCHHHCCCCCCCCCEEEEEEEEEECCCC
VGDVKPVALERGEAAVLYVTGNGSNLSPVWVKRPVSTR
CCCCCCEEECCCCEEEEEEECCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
TFTTTPRRLAKRFALVAGLSVLSVQAFAGGDAALYGPTAPKGSTFVRVYNASNAEVSAT
CCCCCHHHHHHHHHHHHCCHHEEEEEECCCCEEEECCCCCCCCEEEEEEECCCCEEEEE
VGSTNLSDVAPLASSDFSFMPGGDYSAKVGSQTLPVKLAGDHYYTLVNNASGAPQLIEEP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHCCC
PFKNKQKSLVRVQNLSDKPLTLKTADGKTEVVPNVAAKGRGEREINPVKVSLALYEGDKK
CCCCCHHHHEEEECCCCCCEEEEECCCCCEECCCHHHCCCCCCCCCEEEEEEEEEECCCC
VGDVKPVALERGEAAVLYVTGNGSNLSPVWVKRPVSTR
CCCCCCEEECCCCEEEEEEECCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12775688 [H]