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The map label for this gene is cysL [H]

Identifier: 77456943

GI number: 77456943

Start: 843733

End: 844614

Strand: Reverse

Name: cysL [H]

Synonym: Pfl01_0715

Alternate gene names: 77456943

Gene position: 844614-843733 (Counterclockwise)

Preceding gene: 77456945

Following gene: 77456942

Centisome position: 13.12

GC content: 64.97

Gene sequence:

>882_bases
ATGCACTTCGACCTCACCGACCTGCGCCTGTACCTGCACATCCTCGACAGCGGCAACATCACCGCCGGTGCTGCACGCAG
TCATCTTTCGCTGGCGGCGGCCAGTGCGCGGATCCGGACGATGGAAGCGTCGCTGGGCACCGAGTTTCTTGAGCGCGGGC
GCCGTGGGGTCACTCCGACGCCGGCCGGCAAGGCACTGGCGCAACATGCCCGGGTGTTGCTGCAACAGGCCGAACGCCTG
CAACAGGATCTGGCCGAATACGCGCGCGGCGTCAAAGGCCAGGTGCGCCTGCTGTGCAACACCACGGCGCTCAGCGAATA
CCTGCCGGAGGTGCTGGCGGATTTCCTGCGTGAGCATCCCAACCTCGACATCGATCTGCAGGAACTGCCTAGCACCCGCA
TCACCCATGCCTTGCGTCAGGGCGCGGCGGATCTGGGCATTGTGTCCGATGCGATCGACACCACTGACTTGCAGACCCGA
GCCTTTCGCGATGATCCGCTGATGCTGATTCTGCCCTTGGATCATCCGCTGTCTGACGCGACCGAAGTCAGTTTCAGCGA
CGCATTGCGTCATGACTTCGTCGGCCTCGACGCAGACAGCGCGCTGGCGGTCTACCTGGAAGAACAGGCCCTGCACAGCG
GCTCGCGGATGCAGATCCGCATCCGCGCCGATGGTTTCAACGGCGTGATGCGCATGGTGGCTCGAGGTGCCGGCCTGGGC
ATCGTACCGCTGGCGGCGGTTCAGCGCGCTGCGCCGCAAGCCTTCAAGACCCTGCCGATGAATGAAGGCTGGGCCAGGCG
CAAACTGTTGCTGTGCGCCCGGGACTTCGCCGCTCTCCCCGCTTATGCCCGCGCCTTGCTCGACGCCTTGACTCTGCCCT
GA

Upstream 100 bases:

>100_bases
ATCTGCGAGTGCGTTCATGGCCACACTTTGCGCCTGCGCGGGTGAACTGAAAATCTGTCATACACTGAGGCAGCCTCTCT
TTCAGCAAGAGCCACGCATC

Downstream 100 bases:

>100_bases
GGGGCAGACTTTACCCTTGGGGTTTCTTCTTCAAGGACAGTGATTATGGGCAAGCGGATTCTGCTGATTCTCGGCCATCC
ATCCAATGAAAGTTTTTGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MHFDLTDLRLYLHILDSGNITAGAARSHLSLAAASARIRTMEASLGTEFLERGRRGVTPTPAGKALAQHARVLLQQAERL
QQDLAEYARGVKGQVRLLCNTTALSEYLPEVLADFLREHPNLDIDLQELPSTRITHALRQGAADLGIVSDAIDTTDLQTR
AFRDDPLMLILPLDHPLSDATEVSFSDALRHDFVGLDADSALAVYLEEQALHSGSRMQIRIRADGFNGVMRMVARGAGLG
IVPLAAVQRAAPQAFKTLPMNEGWARRKLLLCARDFAALPAYARALLDALTLP

Sequences:

>Translated_293_residues
MHFDLTDLRLYLHILDSGNITAGAARSHLSLAAASARIRTMEASLGTEFLERGRRGVTPTPAGKALAQHARVLLQQAERL
QQDLAEYARGVKGQVRLLCNTTALSEYLPEVLADFLREHPNLDIDLQELPSTRITHALRQGAADLGIVSDAIDTTDLQTR
AFRDDPLMLILPLDHPLSDATEVSFSDALRHDFVGLDADSALAVYLEEQALHSGSRMQIRIRADGFNGVMRMVARGAGLG
IVPLAAVQRAAPQAFKTLPMNEGWARRKLLLCARDFAALPAYARALLDALTLP
>Mature_293_residues
MHFDLTDLRLYLHILDSGNITAGAARSHLSLAAASARIRTMEASLGTEFLERGRRGVTPTPAGKALAQHARVLLQQAERL
QQDLAEYARGVKGQVRLLCNTTALSEYLPEVLADFLREHPNLDIDLQELPSTRITHALRQGAADLGIVSDAIDTTDLQTR
AFRDDPLMLILPLDHPLSDATEVSFSDALRHDFVGLDADSALAVYLEEQALHSGSRMQIRIRADGFNGVMRMVARGAGLG
IVPLAAVQRAAPQAFKTLPMNEGWARRKLLLCARDFAALPAYARALLDALTLP

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=285, Percent_Identity=23.1578947368421, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=241, Percent_Identity=26.1410788381743, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=25.5972696245734, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787879, Length=282, Percent_Identity=23.4042553191489, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788297, Length=170, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI2367136, Length=252, Percent_Identity=26.984126984127, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=23.265306122449, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=279, Percent_Identity=21.1469534050179, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788481, Length=178, Percent_Identity=25.8426966292135, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787601, Length=144, Percent_Identity=25, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=21.5139442231076, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789440, Length=121, Percent_Identity=28.099173553719, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788296, Length=230, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31986; Mature: 31986

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFDLTDLRLYLHILDSGNITAGAARSHLSLAAASARIRTMEASLGTEFLERGRRGVTPT
CCCCHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAGKALAQHARVLLQQAERLQQDLAEYARGVKGQVRLLCNTTALSEYLPEVLADFLREHP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCC
NLDIDLQELPSTRITHALRQGAADLGIVSDAIDTTDLQTRAFRDDPLMLILPLDHPLSDA
CCCEEHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCCCH
TEVSFSDALRHDFVGLDADSALAVYLEEQALHSGSRMQIRIRADGFNGVMRMVARGAGLG
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCCC
IVPLAAVQRAAPQAFKTLPMNEGWARRKLLLCARDFAALPAYARALLDALTLP
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFDLTDLRLYLHILDSGNITAGAARSHLSLAAASARIRTMEASLGTEFLERGRRGVTPT
CCCCHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAGKALAQHARVLLQQAERLQQDLAEYARGVKGQVRLLCNTTALSEYLPEVLADFLREHP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCC
NLDIDLQELPSTRITHALRQGAADLGIVSDAIDTTDLQTRAFRDDPLMLILPLDHPLSDA
CCCEEHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCCCH
TEVSFSDALRHDFVGLDADSALAVYLEEQALHSGSRMQIRIRADGFNGVMRMVARGAGLG
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCCC
IVPLAAVQRAAPQAFKTLPMNEGWARRKLLLCARDFAALPAYARALLDALTLP
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]