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The map label for this gene is rutF [H]

Identifier: 77456940

GI number: 77456940

Start: 842027

End: 842536

Strand: Reverse

Name: rutF [H]

Synonym: Pfl01_0712

Alternate gene names: 77456940

Gene position: 842536-842027 (Counterclockwise)

Preceding gene: 77456941

Following gene: 77456939

Centisome position: 13.09

GC content: 59.41

Gene sequence:

>510_bases
ATGGTTGATTCAACGGAGTTTCGTAATGCAATGGCGATGCTGGGGAGTGCCGTCACCATCGTCACGACCGATGGCCCTGC
GGGGCGTTTCGGCTTTACCGCGTCGGCGGTGTGCAGCGTGACGGATTCACCGCCCACGCTGCTGGTATGCGTCAATCGCT
CGTCGTCCAATCACGAGCATTTCAAGACCAATGGCGTGCTGTGCGTGAACGTCCTGACCGGCGATCACCAGTCGACCTCC
GGTGCGTTCGCCAATCGGCAGTTGAGCATGCAGGAGCGCTTTTCCAGCGTGAATTGCCAGACCCTCAAGACCGGTTCGCC
GGTAATCGAGGAGGCGCTGGTCAGCCTCGATTGCCGAGTCAGCAAGGTCGACGAGGTCGGCACCCACAGCATCTTCTACT
GTGAAATTCTCGAGCTGCAGCAACACAGCGAGGCCACGGAAGGCCTGGTCTATTTCAACCGCAGTTACTACCGTCTGAAC
GACGAACTGCGCCTGGCACCCGAGCAGTGA

Upstream 100 bases:

>100_bases
CCAGAGCGAACGTGTTGCCCGATACCCGTTTTCGCACAACGACAACGGACAAGGAAGAACGGATCGGCTCCATTAAACCT
ACATGGAATGAGGTGACGCG

Downstream 100 bases:

>100_bases
TCCAGACACCCGCCGGCTTCGGCTGAAGCCTGCCGGCGGGTCATGACAGGGATGTAACCATGCTATTCAATGAAGACAAC
TTCGCGGATATCGACCTGAA

Product: flavin reductase-like protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MVDSTEFRNAMAMLGSAVTIVTTDGPAGRFGFTASAVCSVTDSPPTLLVCVNRSSSNHEHFKTNGVLCVNVLTGDHQSTS
GAFANRQLSMQERFSSVNCQTLKTGSPVIEEALVSLDCRVSKVDEVGTHSIFYCEILELQQHSEATEGLVYFNRSYYRLN
DELRLAPEQ

Sequences:

>Translated_169_residues
MVDSTEFRNAMAMLGSAVTIVTTDGPAGRFGFTASAVCSVTDSPPTLLVCVNRSSSNHEHFKTNGVLCVNVLTGDHQSTS
GAFANRQLSMQERFSSVNCQTLKTGSPVIEEALVSLDCRVSKVDEVGTHSIFYCEILELQQHSEATEGLVYFNRSYYRLN
DELRLAPEQ
>Mature_169_residues
MVDSTEFRNAMAMLGSAVTIVTTDGPAGRFGFTASAVCSVTDSPPTLLVCVNRSSSNHEHFKTNGVLCVNVLTGDHQSTS
GAFANRQLSMQERFSSVNCQTLKTGSPVIEEALVSLDCRVSKVDEVGTHSIFYCEILELQQHSEATEGLVYFNRSYYRLN
DELRLAPEQ

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=159, Percent_Identity=52.8301886792453, Blast_Score=170, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18490; Mature: 18490

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDSTEFRNAMAMLGSAVTIVTTDGPAGRFGFTASAVCSVTDSPPTLLVCVNRSSSNHEH
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCE
FKTNGVLCVNVLTGDHQSTSGAFANRQLSMQERFSSVNCQTLKTGSPVIEEALVSLDCRV
EECCCEEEEEEEECCCCCCCCCCCCCEECHHHHHCCCCCEEEECCCHHHHHHHHHHCCCE
SKVDEVGTHSIFYCEILELQQHSEATEGLVYFNRSYYRLNDELRLAPEQ
ECCCCCCCCEEEEEEEEEHHHCCCCCCCEEEEECEEEEECCEEEECCCC
>Mature Secondary Structure
MVDSTEFRNAMAMLGSAVTIVTTDGPAGRFGFTASAVCSVTDSPPTLLVCVNRSSSNHEH
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCE
FKTNGVLCVNVLTGDHQSTSGAFANRQLSMQERFSSVNCQTLKTGSPVIEEALVSLDCRV
EECCCEEEEEEEECCCCCCCCCCCCCEECHHHHHCCCCCEEEECCCHHHHHHHHHHCCCE
SKVDEVGTHSIFYCEILELQQHSEATEGLVYFNRSYYRLNDELRLAPEQ
ECCCCCCCCEEEEEEEEEHHHCCCCCCCEEEEECEEEEECCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA