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The map label for this gene is dusB [H]

Identifier: 77456843

GI number: 77456843

Start: 723085

End: 724095

Strand: Reverse

Name: dusB [H]

Synonym: Pfl01_0615

Alternate gene names: 77456843

Gene position: 724095-723085 (Counterclockwise)

Preceding gene: 77456844

Following gene: 77456842

Centisome position: 11.25

GC content: 62.41

Gene sequence:

>1011_bases
ATGTCGGCGGTACGCATCGGCCCATATACATTGCAGAACGGCTTGATCCTCGCCCCGATGGCGGGTGTCACCGACCAGCC
CTTTCGTCAGCTGTGCAAGCGTTTGGGCGCAGGGCTTGTAGTGTCGGAAATGGTCACCAGTGACATGAGCCTGTGGAACA
CCCGCAAGTCGCGCATGCGCATGATCCACGAAGGCGATCCCGAGCCGCGCTCGGTGCAGATCGCCGGTGGCGATGCGCAG
ATGCTGGCGGATGCGGCCCGGGCGAACGTCGAACTGGGCGCACAGATTATTGATATCAACATGGGTTGCCCGGCGAAGAA
GGTCTGCAACAAGGCCGCCGGCTCCGCGTTGTTGAAGGATGAGGCACTGGTTACCGAGATCCTGAACGCCGTTGTGGCGG
CGGTTGATGTGCCGGTGACCCTGAAGATCCGCACCGGATGGGATCGCGACAACAAGAACGGCCTGACCGTGGCGAAGATC
GCCGAGCAGGCCGGGATCACGGCGCTGGCGGTGCATGGCCGCACCCGCGCCGACCTGTACACCGGTGAAGCCGAGTACGA
CACCATCGCCGCGATCAAGCAGGCGGTGTCGATTCCGGTCTTTGCCAACGGCGACATCGTATCGCCGGAAAAGGCCCGCT
ACGTGCTCGACGCGACCGGTGCCGATGGCCTGTTGATCGGCCGGGCTGCCCAGGGGCGGCCATGGATTTTTCGCGAGATC
GAACACTTCCTGCGTACCGGCGAAAAACTGCCGGCGCCGGAGCTGATCGAGGTGGAACGCATTCTGCTTGAGCATCTGGC
CGCCCTTCACGCCTTCTATGGGGACGTGATGGGTGTGCGAATTGCCCGCAAGCATGTGGGCTGGTATCTCGCAACCTTGC
CGGGCGCCAGGGAATTTCGCGCCCACTTCAATCGTTTGGATGGTACGGAAACACAATGCGCCAACGTTCGGGAGTTCTTC
GCCGAACGTTACAAGAGCCTGACAGGGGACGGAGAAGGGGTGGCCGCATGA

Upstream 100 bases:

>100_bases
AGATTTTGTTCAATTCAGCCTTTATCCAGTCATCGAGAGCGGGTATCATGCCAACCCTTTTTCGAACTCTCATGATCCGG
CCCCACTTCAGGGAAGTCCT

Downstream 100 bases:

>100_bases
CGATGATGACCGAGACTTTAGTGAGTGGAACAACACCCGTGAGCGACAACGTGAATTTGAAACAGCACCTCAATACCCCG
AGCGAAGAAGGCCAGACCCT

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSAVRIGPYTLQNGLILAPMAGVTDQPFRQLCKRLGAGLVVSEMVTSDMSLWNTRKSRMRMIHEGDPEPRSVQIAGGDAQ
MLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKDEALVTEILNAVVAAVDVPVTLKIRTGWDRDNKNGLTVAKI
AEQAGITALAVHGRTRADLYTGEAEYDTIAAIKQAVSIPVFANGDIVSPEKARYVLDATGADGLLIGRAAQGRPWIFREI
EHFLRTGEKLPAPELIEVERILLEHLAALHAFYGDVMGVRIARKHVGWYLATLPGAREFRAHFNRLDGTETQCANVREFF
AERYKSLTGDGEGVAA

Sequences:

>Translated_336_residues
MSAVRIGPYTLQNGLILAPMAGVTDQPFRQLCKRLGAGLVVSEMVTSDMSLWNTRKSRMRMIHEGDPEPRSVQIAGGDAQ
MLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKDEALVTEILNAVVAAVDVPVTLKIRTGWDRDNKNGLTVAKI
AEQAGITALAVHGRTRADLYTGEAEYDTIAAIKQAVSIPVFANGDIVSPEKARYVLDATGADGLLIGRAAQGRPWIFREI
EHFLRTGEKLPAPELIEVERILLEHLAALHAFYGDVMGVRIARKHVGWYLATLPGAREFRAHFNRLDGTETQCANVREFF
AERYKSLTGDGEGVAA
>Mature_335_residues
SAVRIGPYTLQNGLILAPMAGVTDQPFRQLCKRLGAGLVVSEMVTSDMSLWNTRKSRMRMIHEGDPEPRSVQIAGGDAQM
LADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKDEALVTEILNAVVAAVDVPVTLKIRTGWDRDNKNGLTVAKIA
EQAGITALAVHGRTRADLYTGEAEYDTIAAIKQAVSIPVFANGDIVSPEKARYVLDATGADGLLIGRAAQGRPWIFREIE
HFLRTGEKLPAPELIEVERILLEHLAALHAFYGDVMGVRIARKHVGWYLATLPGAREFRAHFNRLDGTETQCANVREFFA
ERYKSLTGDGEGVAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=292, Percent_Identity=29.7945205479452, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI239788462, Length=292, Percent_Identity=29.7945205479452, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=34.5132743362832, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=29.9559471365639, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI8923374, Length=246, Percent_Identity=32.520325203252, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=60.2523659305994, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1788462, Length=258, Percent_Identity=33.7209302325581, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI145693211, Length=301, Percent_Identity=24.2524916943522, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=243, Percent_Identity=31.6872427983539, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=288, Percent_Identity=29.5138888888889, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=30.8641975308642, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=251, Percent_Identity=31.4741035856574, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=270, Percent_Identity=28.5185185185185, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=30, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=27.3913043478261, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=32.7433628318584, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=32.7433628318584, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585320, Length=237, Percent_Identity=31.2236286919831, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45549423, Length=262, Percent_Identity=31.2977099236641, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19921524, Length=316, Percent_Identity=27.2151898734177, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36398; Mature: 36267

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVRIGPYTLQNGLILAPMAGVTDQPFRQLCKRLGAGLVVSEMVTSDMSLWNTRKSRMR
CCCEEECCEEECCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MIHEGDPEPRSVQIAGGDAQMLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKD
HHHCCCCCCCEEEEECCHHHHHHHHHHCCCEECCEEEEEECCCCHHHHHHHHHCCHHHHH
EALVTEILNAVVAAVDVPVTLKIRTGWDRDNKNGLTVAKIAEQAGITALAVHGRTRADLY
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEE
TGEAEYDTIAAIKQAVSIPVFANGDIVSPEKARYVLDATGADGLLIGRAAQGRPWIFREI
CCCCCHHHHHHHHHHHCCCEECCCCEECCCCCEEEEECCCCCCEEEECCCCCCCHHHHHH
EHFLRTGEKLPAPELIEVERILLEHLAALHAFYGDVMGVRIARKHVGWYLATLPGAREFR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHH
AHFNRLDGTETQCANVREFFAERYKSLTGDGEGVAA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAVRIGPYTLQNGLILAPMAGVTDQPFRQLCKRLGAGLVVSEMVTSDMSLWNTRKSRMR
CCEEECCEEECCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MIHEGDPEPRSVQIAGGDAQMLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKD
HHHCCCCCCCEEEEECCHHHHHHHHHHCCCEECCEEEEEECCCCHHHHHHHHHCCHHHHH
EALVTEILNAVVAAVDVPVTLKIRTGWDRDNKNGLTVAKIAEQAGITALAVHGRTRADLY
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEE
TGEAEYDTIAAIKQAVSIPVFANGDIVSPEKARYVLDATGADGLLIGRAAQGRPWIFREI
CCCCCHHHHHHHHHHHCCCEECCCCEECCCCCEEEEECCCCCCEEEECCCCCCCHHHHHH
EHFLRTGEKLPAPELIEVERILLEHLAALHAFYGDVMGVRIARKHVGWYLATLPGAREFR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHH
AHFNRLDGTETQCANVREFFAERYKSLTGDGEGVAA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]