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The map label for this gene is purH [H]

Identifier: 77456841

GI number: 77456841

Start: 721078

End: 722697

Strand: Reverse

Name: purH [H]

Synonym: Pfl01_0613

Alternate gene names: 77456841

Gene position: 722697-721078 (Counterclockwise)

Preceding gene: 77456842

Following gene: 77456840

Centisome position: 11.22

GC content: 62.47

Gene sequence:

>1620_bases
ATGGAAATTGAGATGACCGACCAGACTACCCGCCTGCCGATCCGCCGCGCCTTGATCAGTGTTTCCGACAAGACCGGGAT
CCTCGAATTCGCCAGGGAGCTGGAAGCCCTGGGCGTGGAAATCCTTTCCACCGGCGGGACCTTCAAACTGCTGCAGGACA
ACGGCGTGGCCGCAGTGGAAGTCGCGGATTACACCGGTTTCGCAGAGATGATGGACGGTCGGGTGAAAACCCTGCACCCG
AAAATCCACGGCGGGATCCTCGGTCGTCGCGGTATCGACGACGCCATCATGAACGAGCACGGCATCAAGCCGATCGATCT
GGTAGCCGTCAACCTGTACCCGTTCGAAGCCACCATCAGCAAGCCAGGCTGCGACCTGCCGACCGCCATCGAGAACATCG
ACATCGGCGGTCCGACCATGGTCCGTTCGGCGGCGAAAAACCACAAAGACGTGGCCATCGTGGTGAATGCCAGCGATTAC
GCCGACGTCCTGGAAAACCTCAAGGCCGGTGGCCTGACCTACGCTCAGCGTTTCGACCTGATGCTCAAGGCGTTCGAACA
CACCGCCGCCTACGACGGCATGATCGCCAACTACATGGGCACCGTGAACCAGGCCGCCGAAACCCTGAGCACCGAAGGTC
GCAGCGAATTCCCGCGCACCTTCAACAGCCAGTTCATCAAGGCCCAGGAAATGCGCTACGGCGAGAACCCGCACCAGAGC
GCGGCGTTCTACGTTGAGGCCAAGCCTGCCGAAGTCGGCATCGCCACCGCCACCCAGTTGCAAGGCAAGGAACTGTCGTA
CAACAACGTGGCCGACACCGACGCCGCGCTGGAATGCGTGAAGAGCTTCGTCAAACCGGCCTGCGTGATCGTCAAGCACG
CCAACCCGTGCGGCGTGGCCGTGAGCCCGGACGCCGAAGGTGGCATCCGTCAGGCCTACGAACTGGCCTACGCCACCGAC
ACCGAATCCGCGTTCGGCGGCATCATCGCCTTCAACCGTGAACTGGATGCCGAGACCGCCAAGGCGATCGTCGAGCGTCA
GTTCGTTGAAGTGATCATCGCCCCAAGTGTCAGCGAAGAAGCCCGCGCCATTGTCGCCGCCAAGGCCAACGTGCGCCTGC
TGGCCTGCGGCGAGTGGTCGGCGGATCGCGTTCCGGCCTGGGACTACAAGCGCGTCAACGGCGGTCTGCTGGTACAGAGC
CGCGACATCGGCATGATCGGTGCCGACGACCTGAAAGTGGTGACCAAGCGCGCACCGACCGAGCAGGAAGTCCACGACCT
GATCTTCGCCTGGAAAGTGGCCAAGTTCGTCAAATCCAACGCCATCGTCTACGCCAAGAACCGCCAGACCATCGGTGTCG
GCGCCGGCCAGATGAGCCGCGTGAACTCGGCGCGTATCGCCGCGATCAAGGCTGAGCACGCCGGTCTGCAAGTGGCCGGT
TCGGTGATGGCATCCGATGCGTTCTTCCCGTTCCGCGACGGCCTGGACAATGCGGCCAAGGTCGGTATCACGGCGGTGAT
CCAGCCAGGCGGCTCGATGCGTGATGCTGAAGTGATTGCTGCTGCTGATGAAGCCGGCATCGCGATGGTCTTCACCGGCA
TGCGCCATTTCCGTCACTGA

Upstream 100 bases:

>100_bases
AAGAAACTCAAGCAGTACGATCTGCTGTAAGCATTCAATCAAACCAGAAAGGCGCCCGCGTAAAACCGGTCGCCTTTTTT
GCTGACTTCCCTTGCTTTTG

Downstream 100 bases:

>100_bases
TAAACCGATTCCCTGTGGGAGCGGGCTTGCCCGCGATGCAGACACCTTGGTTCTTCAGATAGACCGCAGTGATGCCATCG
CTGGCAAGCCAGCTCCCACA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVEVADYTGFAEMMDGRVKTLHP
KIHGGILGRRGIDDAIMNEHGIKPIDLVAVNLYPFEATISKPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDY
ADVLENLKAGGLTYAQRFDLMLKAFEHTAAYDGMIANYMGTVNQAAETLSTEGRSEFPRTFNSQFIKAQEMRYGENPHQS
AAFYVEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVAVSPDAEGGIRQAYELAYATD
TESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEEARAIVAAKANVRLLACGEWSADRVPAWDYKRVNGGLLVQS
RDIGMIGADDLKVVTKRAPTEQEVHDLIFAWKVAKFVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVAG
SVMASDAFFPFRDGLDNAAKVGITAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH

Sequences:

>Translated_539_residues
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVEVADYTGFAEMMDGRVKTLHP
KIHGGILGRRGIDDAIMNEHGIKPIDLVAVNLYPFEATISKPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDY
ADVLENLKAGGLTYAQRFDLMLKAFEHTAAYDGMIANYMGTVNQAAETLSTEGRSEFPRTFNSQFIKAQEMRYGENPHQS
AAFYVEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVAVSPDAEGGIRQAYELAYATD
TESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEEARAIVAAKANVRLLACGEWSADRVPAWDYKRVNGGLLVQS
RDIGMIGADDLKVVTKRAPTEQEVHDLIFAWKVAKFVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVAG
SVMASDAFFPFRDGLDNAAKVGITAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH
>Mature_539_residues
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVEVADYTGFAEMMDGRVKTLHP
KIHGGILGRRGIDDAIMNEHGIKPIDLVAVNLYPFEATISKPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDY
ADVLENLKAGGLTYAQRFDLMLKAFEHTAAYDGMIANYMGTVNQAAETLSTEGRSEFPRTFNSQFIKAQEMRYGENPHQS
AAFYVEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVAVSPDAEGGIRQAYELAYATD
TESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEEARAIVAAKANVRLLACGEWSADRVPAWDYKRVNGGLLVQS
RDIGMIGADDLKVVTKRAPTEQEVHDLIFAWKVAKFVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVAG
SVMASDAFFPFRDGLDNAAKVGITAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=512, Percent_Identity=36.1328125, Blast_Score=258, Evalue=9e-69,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=69.6798493408663, Blast_Score=757, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=522, Percent_Identity=32.5670498084291, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=355, Percent_Identity=26.4788732394366, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=63, Percent_Identity=50.7936507936508, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=490, Percent_Identity=35.5102040816327, Blast_Score=246, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6323056, Length=497, Percent_Identity=35.2112676056338, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24649832, Length=507, Percent_Identity=36.094674556213, Blast_Score=250, Evalue=2e-66,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57942; Mature: 57942

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVE
CEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCEEEEEECCCEEEEE
VADYTGFAEMMDGRVKTLHPKIHGGILGRRGIDDAIMNEHGIKPIDLVAVNLYPFEATIS
EECCCCHHHHHCCCCEEECCHHHCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECC
KPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDYADVLENLKAGGLTYAQRFDL
CCCCCCCCHHHCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHH
MLKAFEHTAAYDGMIANYMGTVNQAAETLSTEGRSEFPRTFNSQFIKAQEMRYGENPHQS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AAFYVEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVA
EEEEEEECCCCCCEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEE
VSPDAEGGIRQAYELAYATDTESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEE
ECCCCCCCHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHH
ARAIVAAKANVRLLACGEWSADRVPAWDYKRVNGGLLVQSRDIGMIGADDLKVVTKRAPT
HHEEEEEECCEEEEEECCCCCCCCCCCCCEECCCCEEEEECCCCEECCCCHHEEECCCCC
EQEVHDLIFAWKVAKFVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVAG
HHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCHHHCCCCEEEEEEECCCCCEEEH
SVMASDAFFPFRDGLDNAAKVGITAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH
HHHHCCCCCCHHCCCCCHHHCCEEEEECCCCCCCCCEEEEECCCCCEEHEEHHHHHHCC
>Mature Secondary Structure
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVE
CEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCEEEEEECCCEEEEE
VADYTGFAEMMDGRVKTLHPKIHGGILGRRGIDDAIMNEHGIKPIDLVAVNLYPFEATIS
EECCCCHHHHHCCCCEEECCHHHCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECC
KPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDYADVLENLKAGGLTYAQRFDL
CCCCCCCCHHHCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHH
MLKAFEHTAAYDGMIANYMGTVNQAAETLSTEGRSEFPRTFNSQFIKAQEMRYGENPHQS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AAFYVEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVA
EEEEEEECCCCCCEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEE
VSPDAEGGIRQAYELAYATDTESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEE
ECCCCCCCHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHH
ARAIVAAKANVRLLACGEWSADRVPAWDYKRVNGGLLVQSRDIGMIGADDLKVVTKRAPT
HHEEEEEECCEEEEEECCCCCCCCCCCCCEECCCCEEEEECCCCEECCCCHHEEECCCCC
EQEVHDLIFAWKVAKFVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVAG
HHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCHHHCCCCEEEEEEECCCCCEEEH
SVMASDAFFPFRDGLDNAAKVGITAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH
HHHHCCCCCCHHCCCCCHHHCCEEEEECCCCCCCCCEEEEECCCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA