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The map label for this gene is purD [H]

Identifier: 77456840

GI number: 77456840

Start: 719632

End: 720924

Strand: Reverse

Name: purD [H]

Synonym: Pfl01_0612

Alternate gene names: 77456840

Gene position: 720924-719632 (Counterclockwise)

Preceding gene: 77456841

Following gene: 77456839

Centisome position: 11.2

GC content: 64.19

Gene sequence:

>1293_bases
ATGAATGTTTTGATCATTGGCAGCGGTGGCCGCGAACACGCCCTGGCCTGGAAAGTGGCTCAGGATCCGCGTGTGCAGAA
AGTTTTCGTCGCACCGGGCAACGCCGGCTCCGCGATTGAAGCCAAGTGCGAGAACGTCGCCATCGATGTGCTGGCCCTCG
AGCAGCTCGCTGACTTCGCCGAGAAAAACGTTTCCCTGACCATCGTCGGTCCGGAAGTGCCATTGGTGGCCGGCGTCGTC
GACCTGTTCCGCTCCCGTGGCCTGGACTGCTTCGGTCCGACCGCTGGCGCCGCGCAGCTGGAAGGCTCGAAAGCCTTCAC
CAAGGATTTCCTGGCACGCCACAAGATCCCGACCGCCGACTACCAGAACTTCACCGAGATCGAGCCGGCCCTGGCTTATC
TGCGTGAAAAAGGCGCACCGATCGTGATCAAGGCCGACGGCCTGGCCGCCGGTAAAGGCGTGATTGTTGCCATGACCCTG
GCCGAAGCCGAAGACGCCGTGCGCGACATGCTCGCCGGCAATGCCTTTGGCGACGCTGGTTCGCGCGTTGTCATCGAAGA
ATTCCTCGACGGCGAAGAAGCCAGCTTCATCGTCATGGTCGACGGCAAGAACGTCCTGCCGATGGCCACCAGCCAGGACC
ACAAACGCGTCGGCGACGGCGACAGCGGCCCGAACACCGGCGGCATGGGTGCTTACTCCCCCGCACCGGTGGTCACCGCC
GACGTGCACCAGCGTGTGATGGACCAGGTGATCTGGCCGACCGTGCGCGGCATGGCCGACGAAGGCAATGTCTACACCGG
TTTCCTGTACGCCGGTCTGATGATCGACAAGGCCGGCAATCCGAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGG
AAACCCAACCGGTGATGCTGCGTCTGCAATCAAGCCTGGTACTGCTGGTCGAAGCCGCGCTGGCTCAGGCGCTGGACAAG
GTTGAAGCGCAGTGGGATCCACGCCCGAGCGTCGGCATCGTGCTGGCCGCCGGCGGTTATCCGGGCGACTATGCCAAGGG
CGCGCCGATCAACGGTCTGGACGCAGCCGCCGCACTGGAAGGCAAGGTCTTCCACGCCGGTACTGCGCTCAAGGACGGCA
ACGTCGTGACGGCCGGTGGTCGGGTGCTTTGCGCCACCGCCATGGGCGCCAGCGTCGGCGCCGCCCAGGAGCAGGCCTAC
AAACTGGCCAAGGCCATCGACTGGGAAGGCTGCTTCTACCGCACCGACATTGGCTACCGCGCCATTGCCCGTGAACGCGG
TGAGTCTCAGTAA

Upstream 100 bases:

>100_bases
TTCAGATAGACCGCAGTGATGCCATCGCTGGCAAGCCAGCTCCCACAGAAAAGCGCCCCAACGGCGCTCAACAGAATTAG
CGTCATCCGAGGTTTTTGAA

Downstream 100 bases:

>100_bases
TAAGCTACGCTGTGTTTCCGGCAGGGGCCCATCGCCCCTGCCGGACTGTTCCGACGCTGCGCATCGTTATATTCTGGCAT
CAACCTACGAAGGGATTTCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVLIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGSAIEAKCENVAIDVLALEQLADFAEKNVSLTIVGPEVPLVAGVV
DLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTADYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
DVHQRVMDQVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVMLRLQSSLVLLVEAALAQALDK
VEAQWDPRPSVGIVLAAGGYPGDYAKGAPINGLDAAAALEGKVFHAGTALKDGNVVTAGGRVLCATAMGASVGAAQEQAY
KLAKAIDWEGCFYRTDIGYRAIARERGESQ

Sequences:

>Translated_430_residues
MNVLIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGSAIEAKCENVAIDVLALEQLADFAEKNVSLTIVGPEVPLVAGVV
DLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTADYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
DVHQRVMDQVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVMLRLQSSLVLLVEAALAQALDK
VEAQWDPRPSVGIVLAAGGYPGDYAKGAPINGLDAAAALEGKVFHAGTALKDGNVVTAGGRVLCATAMGASVGAAQEQAY
KLAKAIDWEGCFYRTDIGYRAIARERGESQ
>Mature_430_residues
MNVLIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGSAIEAKCENVAIDVLALEQLADFAEKNVSLTIVGPEVPLVAGVV
DLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTADYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
DVHQRVMDQVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVMLRLQSSLVLLVEAALAQALDK
VEAQWDPRPSVGIVLAAGGYPGDYAKGAPINGLDAAAALEGKVFHAGTALKDGNVVTAGGRVLCATAMGASVGAAQEQAY
KLAKAIDWEGCFYRTDIGYRAIARERGESQ

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=53.0660377358491, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=53.0660377358491, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=53.0660377358491, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=52.9411764705882, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=73.5981308411215, Blast_Score=636, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=43.4272300469484, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=41.9134396355353, Blast_Score=335, Evalue=7e-93,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.3488372093023, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.3488372093023, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45149; Mature: 45149

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGSAIEAKCENVAIDVLALEQLADFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEECCCCEEEHHHHHHHHHHHH
EKNVSLTIVGPEVPLVAGVVDLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTAD
HCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCC
YQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLAGNAFGDAG
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
SRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
CEEEHHHHCCCCCCEEEEEECCCCEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEH
DVHQRVMDQVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVML
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEE
RLQSSLVLLVEAALAQALDKVEAQWDPRPSVGIVLAAGGYPGDYAKGAPINGLDAAAALE
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHC
GKVFHAGTALKDGNVVTAGGRVLCATAMGASVGAAQEQAYKLAKAIDWEGCFYRTDIGYR
CEEEECCCEECCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCHH
AIARERGESQ
HHHHHCCCCC
>Mature Secondary Structure
MNVLIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGSAIEAKCENVAIDVLALEQLADFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEECCCCEEEHHHHHHHHHHHH
EKNVSLTIVGPEVPLVAGVVDLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTAD
HCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCC
YQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLAGNAFGDAG
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
SRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
CEEEHHHHCCCCCCEEEEEECCCCEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEH
DVHQRVMDQVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVML
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEE
RLQSSLVLLVEAALAQALDKVEAQWDPRPSVGIVLAAGGYPGDYAKGAPINGLDAAAALE
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHC
GKVFHAGTALKDGNVVTAGGRVLCATAMGASVGAAQEQAYKLAKAIDWEGCFYRTDIGYR
CEEEECCCEECCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCHH
AIARERGESQ
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]