Click here to switch to the map view.

The map label for this gene is rfaG [H]

Identifier: 77456695

GI number: 77456695

Start: 541536

End: 542657

Strand: Direct

Name: rfaG [H]

Synonym: Pfl01_0467

Alternate gene names: 77456695

Gene position: 541536-542657 (Clockwise)

Preceding gene: 77456694

Following gene: 77456696

Centisome position: 8.41

GC content: 61.41

Gene sequence:

>1122_bases
ATGCAATTGGCTTTTGTCCTGTACAAGTATTTCCCGTTCGGCGGTTTGCAGCGCGACTTCATGCGCATCGCCCTCGAATG
CCAGAAGCGCGGCCATCAAATTCGCGTCTACACCCTGATCTGGGAAGGCGACGTGCCGCCCGGTTTCGAAGTGCTGGTGG
CGCCGGTCAAGGCGTTCTTCAATCACCGGCGCAATGAAAAGCTCAGCGCGTGGATGGAAGCGGACCTGGCCAAGCGTCCA
GTGGATCGCCTGATCGGCTTCAACAAAATGCCGGGGCTGGACGTCTACTACGCCGCCGACGGCTGCTTCGAAGACAAGGC
GCAGAACCTGCGCAATTCCCTGTATCGCCGCTGGGGCCGTTATCGCCACTTCGCTGAATACGAGCGCGCGGTGTTCGCCA
AGGATGCGAAGACTGAAGTGCTGATGATTTCCGAAGTGCAGCAGCCGCTGTTCATCAAGTATTACGACACGCCGCTGGAA
CGCTTCCACCTGCTGCCGCCGGGCATTGCCCAGGATCGTCGCCGTCCGGCGGATGCCGACCAGATTCGCGCCGGTTTCCG
CGCCGAATTCAATCTGAAGGACGACGAACTGCTGCTGGTGCAGATCGGCTCCGGGTTCAAGACCAAGGGTGTCGATCGCA
GCCTCAAAACACTGGCCGCATTGCCCGCCGAGCTGAAGAAACGCACCCGGCTGTTTGTAATCGGCCAGGATGACCCCAAA
TTGTTCCAGATGCAGAGTGCGACGCTGGGCCTGGGCGACAACGTGACGTTCCTCAAGGGGCGCAGCGATATCCCGCGTTT
CCTGCTCGGCGCCGATCTGTTGATCCACCCGGCGTACAACGAAAACACCGGTACGGTGCTGCTTGAAGCGCTGGTCGCCG
GGTTGCCGGTGCTGGTCAGCGCGGTCTGCGGTTACGCCCATTACATCGCCGAGGCCGACGCCGGACGAGTGCTGGACGAG
CCGTTCGATCAGGCGCAACTGACGCAATACCTGACTGACATGTTGAACGACGACGCTGCACGGGCGGCCTGGAGCCGCAA
CGGTCTGGCCTTCGCCGAGACGGCCGACCTCTACAGCATGCCGCAGCACGCGGCCGATGTGATTCTGGCGGAGCACGCTT
AA

Upstream 100 bases:

>100_bases
TTGACCTGAAACGCGAGTGGCCTCTGTGCTTCACGCGTCTGAATCCCGAGCGTGTTGCCAGCCGACTGAGCACGTTGTTG
ATGGCTGAGGAGCTGCGCTG

Downstream 100 bases:

>100_bases
ATGAAGTTGATGCTGGCTGAACCGTTCAAGAGCCTGTGGGCCGGACGCGATCCGTTCGCCGAGGTCGAGGGCTTGCAGGG
CGAGGTGTACCGCGAACTGG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: Glucosyltransferase I [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MQLAFVLYKYFPFGGLQRDFMRIALECQKRGHQIRVYTLIWEGDVPPGFEVLVAPVKAFFNHRRNEKLSAWMEADLAKRP
VDRLIGFNKMPGLDVYYAADGCFEDKAQNLRNSLYRRWGRYRHFAEYERAVFAKDAKTEVLMISEVQQPLFIKYYDTPLE
RFHLLPPGIAQDRRRPADADQIRAGFRAEFNLKDDELLLVQIGSGFKTKGVDRSLKTLAALPAELKKRTRLFVIGQDDPK
LFQMQSATLGLGDNVTFLKGRSDIPRFLLGADLLIHPAYNENTGTVLLEALVAGLPVLVSAVCGYAHYIAEADAGRVLDE
PFDQAQLTQYLTDMLNDDAARAAWSRNGLAFAETADLYSMPQHAADVILAEHA

Sequences:

>Translated_373_residues
MQLAFVLYKYFPFGGLQRDFMRIALECQKRGHQIRVYTLIWEGDVPPGFEVLVAPVKAFFNHRRNEKLSAWMEADLAKRP
VDRLIGFNKMPGLDVYYAADGCFEDKAQNLRNSLYRRWGRYRHFAEYERAVFAKDAKTEVLMISEVQQPLFIKYYDTPLE
RFHLLPPGIAQDRRRPADADQIRAGFRAEFNLKDDELLLVQIGSGFKTKGVDRSLKTLAALPAELKKRTRLFVIGQDDPK
LFQMQSATLGLGDNVTFLKGRSDIPRFLLGADLLIHPAYNENTGTVLLEALVAGLPVLVSAVCGYAHYIAEADAGRVLDE
PFDQAQLTQYLTDMLNDDAARAAWSRNGLAFAETADLYSMPQHAADVILAEHA
>Mature_373_residues
MQLAFVLYKYFPFGGLQRDFMRIALECQKRGHQIRVYTLIWEGDVPPGFEVLVAPVKAFFNHRRNEKLSAWMEADLAKRP
VDRLIGFNKMPGLDVYYAADGCFEDKAQNLRNSLYRRWGRYRHFAEYERAVFAKDAKTEVLMISEVQQPLFIKYYDTPLE
RFHLLPPGIAQDRRRPADADQIRAGFRAEFNLKDDELLLVQIGSGFKTKGVDRSLKTLAALPAELKKRTRLFVIGQDDPK
LFQMQSATLGLGDNVTFLKGRSDIPRFLLGADLLIHPAYNENTGTVLLEALVAGLPVLVSAVCGYAHYIAEADAGRVLDE
PFDQAQLTQYLTDMLNDDAARAAWSRNGLAFAETADLYSMPQHAADVILAEHA

Specific function: Involved in the addition of the first glucose residue to the lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790061, Length=368, Percent_Identity=50.8152173913043, Blast_Score=396, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 42224; Mature: 42224

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLAFVLYKYFPFGGLQRDFMRIALECQKRGHQIRVYTLIWEGDVPPGFEVLVAPVKAFF
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
NHRRNEKLSAWMEADLAKRPVDRLIGFNKMPGLDVYYAADGCFEDKAQNLRNSLYRRWGR
HCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
YRHFAEYERAVFAKDAKTEVLMISEVQQPLFIKYYDTPLERFHLLPPGIAQDRRRPADAD
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCHHHHEECCCCCHHHHCCCCCCHH
QIRAGFRAEFNLKDDELLLVQIGSGFKTKGVDRSLKTLAALPAELKKRTRLFVIGQDDPK
HHHCCCEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH
LFQMQSATLGLGDNVTFLKGRSDIPRFLLGADLLIHPAYNENTGTVLLEALVAGLPVLVS
HEEEHHCCCCCCCCEEEECCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AVCGYAHYIAEADAGRVLDEPFDQAQLTQYLTDMLNDDAARAAWSRNGLAFAETADLYSM
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECHHHHHC
PQHAADVILAEHA
CHHHHHEEEECCC
>Mature Secondary Structure
MQLAFVLYKYFPFGGLQRDFMRIALECQKRGHQIRVYTLIWEGDVPPGFEVLVAPVKAFF
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
NHRRNEKLSAWMEADLAKRPVDRLIGFNKMPGLDVYYAADGCFEDKAQNLRNSLYRRWGR
HCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
YRHFAEYERAVFAKDAKTEVLMISEVQQPLFIKYYDTPLERFHLLPPGIAQDRRRPADAD
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCHHHHEECCCCCHHHHCCCCCCHH
QIRAGFRAEFNLKDDELLLVQIGSGFKTKGVDRSLKTLAALPAELKKRTRLFVIGQDDPK
HHHCCCEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH
LFQMQSATLGLGDNVTFLKGRSDIPRFLLGADLLIHPAYNENTGTVLLEALVAGLPVLVS
HEEEHHCCCCCCCCEEEECCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AVCGYAHYIAEADAGRVLDEPFDQAQLTQYLTDMLNDDAARAAWSRNGLAFAETADLYSM
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECHHHHHC
PQHAADVILAEHA
CHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 8041620; 9278503; 1447141; 7988890 [H]