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The map label for this gene is nisX1 [H]

Identifier: 77454803

GI number: 77454803

Start: 217450

End: 217887

Strand: Direct

Name: nisX1 [H]

Synonym: pREL1_0236

Alternate gene names: 77454803

Gene position: 217450-217887 (Clockwise)

Preceding gene: 77454800

Following gene: 77454806

Centisome position: 80.07

GC content: 55.02

Gene sequence:

>438_bases
TTGGACTGTTTCAGCAAGATGATCGTAGGTCGTTCTTTCTCCACGACTGCTGATACGGCACTGGTCAACAATGCGGTGAA
CATGGCCGCGCAGGAACGGATCCGTTGGGAGGCAACGATCTTGCATGCCGATCATGGTCCCCAGTTCACGTCGTGGGGGT
TCGGTGAGAATCTGCGGAGGTGGGGATTGCTCGAGTCTTTCGGGACGGTCGGTGATTGCTTCGATAACGCGGTCATGGAG
GCATTCTGGGGACGTTTGCAGACGGAATTGCTGAACACGAAGAAGTGGTCGACCACGCTGGAGTTGACGGCAGCCATGGC
CGATCATATCGACAATTTTCATAATGCCGAACGGCGCCACAGCTATCTCGGCAACCTCAGCCCGATGGAGTATGAGAAGC
TCTGGGCAGACATCCAACCGTATCCTCAGCTCTCATAA

Upstream 100 bases:

>100_bases
CCGGGCCTGGAATTCGCACTGAACGTCAACGCCGCCCTCCCTGTCGGGTGGCATTACGGAGCATCCCGCTCGGGACGGAG
AGGTTTACTGCTGTGCGATT

Downstream 100 bases:

>100_bases
CATTGGTGCACTATTGGGGGCACGGATCAAATCAGAGAATCAATGCGTGTGAATACGGCTGGCAGGGCTTCGAGGAGGCT
GTGGAATTCGTTCATGTTCA

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MDCFSKMIVGRSFSTTADTALVNNAVNMAAQERIRWEATILHADHGPQFTSWGFGENLRRWGLLESFGTVGDCFDNAVME
AFWGRLQTELLNTKKWSTTLELTAAMADHIDNFHNAERRHSYLGNLSPMEYEKLWADIQPYPQLS

Sequences:

>Translated_145_residues
MDCFSKMIVGRSFSTTADTALVNNAVNMAAQERIRWEATILHADHGPQFTSWGFGENLRRWGLLESFGTVGDCFDNAVME
AFWGRLQTELLNTKKWSTTLELTAAMADHIDNFHNAERRHSYLGNLSPMEYEKLWADIQPYPQLS
>Mature_145_residues
MDCFSKMIVGRSFSTTADTALVNNAVNMAAQERIRWEATILHADHGPQFTSWGFGENLRRWGLLESFGTVGDCFDNAVME
AFWGRLQTELLNTKKWSTTLELTAAMADHIDNFHNAERRHSYLGNLSPMEYEKLWADIQPYPQLS

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=132, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787263, Length=132, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786753, Length=132, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786570, Length=132, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786490, Length=132, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789981, Length=131, Percent_Identity=29.7709923664122, Blast_Score=73, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 16562; Mature: 16562

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCFSKMIVGRSFSTTADTALVNNAVNMAAQERIRWEATILHADHGPQFTSWGFGENLRR
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHH
WGLLESFGTVGDCFDNAVMEAFWGRLQTELLNTKKWSTTLELTAAMADHIDNFHNAERRH
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SYLGNLSPMEYEKLWADIQPYPQLS
HHHCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDCFSKMIVGRSFSTTADTALVNNAVNMAAQERIRWEATILHADHGPQFTSWGFGENLRR
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHH
WGLLESFGTVGDCFDNAVMEAFWGRLQTELLNTKKWSTTLELTAAMADHIDNFHNAERRH
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SYLGNLSPMEYEKLWADIQPYPQLS
HHHCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]