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The map label for this gene is ptsN [H]

Identifier: 75674378

GI number: 75674378

Start: 204654

End: 205115

Strand: Direct

Name: ptsN [H]

Synonym: Nwi_0179

Alternate gene names: 75674378

Gene position: 204654-205115 (Clockwise)

Preceding gene: 75674377

Following gene: 75674382

Centisome position: 6.02

GC content: 63.2

Gene sequence:

>462_bases
ATGATGATTACGGATCTGGTCGCACCCGAGGCGATCCTTCCCGCTTTGAAAGTCACCAGCAAAAAGCAGGCGCTGCAGGA
ACTGGCGGCGCGCGCTGCGGAACTCACAGGGCAGAACGACCGCGCGATCTTCGAGGTGCTGCTGCGGCGCGAGAAGCTCG
GGACCACCGCGGTCGGCTATGGCGTTGCGATTCCGCATGGCAAGCTGCAGAAGCTTGAGAAGATTTTCGGTCTTTTCGCG
CGACTCGAACGTTCGATCGATTTCGAAGCGATGGACGGGCAGCCGGTCGATCTGATCTTTTTGCTGCTCGCTCCCGAAGG
CGCGGGCGCGGATCATCTGAAGGCGCTGGCGCGCATCGCACGGCTCTTGCGCGATCAGGACATCGCCAACAAGCTGCGCG
CCTCGCGCGACGCGGAGGCGATCTATTCCGTGCTGGCGCTGCCGCCAGCGACGGTCCCCTGA

Upstream 100 bases:

>100_bases
CGCTTACCGTGTGCGTTCGCCCGTATGTGGGCTCACGTTTGTGCTGCTTGTCTGACGTGTCTTTCGCAACCTGACGGCAA
ACCTCACCTCGGAACGTCAT

Downstream 100 bases:

>100_bases
GCTGTTGTTGAATTAGGCCGGATCGACATTCACGGCAGCCAGATCAGGGCTGCGGCCAGGTGAACGAAGCCCGTGAAGTG
AACGGTTCGCCTGTCGTAGC

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MMITDLVAPEAILPALKVTSKKQALQELAARAAELTGQNDRAIFEVLLRREKLGTTAVGYGVAIPHGKLQKLEKIFGLFA
RLERSIDFEAMDGQPVDLIFLLLAPEGAGADHLKALARIARLLRDQDIANKLRASRDAEAIYSVLALPPATVP

Sequences:

>Translated_153_residues
MMITDLVAPEAILPALKVTSKKQALQELAARAAELTGQNDRAIFEVLLRREKLGTTAVGYGVAIPHGKLQKLEKIFGLFA
RLERSIDFEAMDGQPVDLIFLLLAPEGAGADHLKALARIARLLRDQDIANKLRASRDAEAIYSVLALPPATVP
>Mature_153_residues
MMITDLVAPEAILPALKVTSKKQALQELAARAAELTGQNDRAIFEVLLRREKLGTTAVGYGVAIPHGKLQKLEKIFGLFA
RLERSIDFEAMDGQPVDLIFLLLAPEGAGADHLKALARIARLLRDQDIANKLRASRDAEAIYSVLALPPATVP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=150, Percent_Identity=38.6666666666667, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI1788726, Length=142, Percent_Identity=31.6901408450704, Blast_Score=69, Evalue=9e-14,
Organism=Escherichia coli, GI1786951, Length=148, Percent_Identity=31.7567567567568, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI2367327, Length=107, Percent_Identity=30.8411214953271, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16607; Mature: 16607

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMITDLVAPEAILPALKVTSKKQALQELAARAAELTGQNDRAIFEVLLRREKLGTTAVGY
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHCC
GVAIPHGKLQKLEKIFGLFARLERSIDFEAMDGQPVDLIFLLLAPEGAGADHLKALARIA
CEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHH
RLLRDQDIANKLRASRDAEAIYSVLALPPATVP
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMITDLVAPEAILPALKVTSKKQALQELAARAAELTGQNDRAIFEVLLRREKLGTTAVGY
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHCC
GVAIPHGKLQKLEKIFGLFARLERSIDFEAMDGQPVDLIFLLLAPEGAGADHLKALARIA
CEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHH
RLLRDQDIANKLRASRDAEAIYSVLALPPATVP
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]