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The map label for this gene is glmU [H]

Identifier: 75674247

GI number: 75674247

Start: 55254

End: 55979

Strand: Direct

Name: glmU [H]

Synonym: Nwi_0048

Alternate gene names: 75674247

Gene position: 55254-55979 (Clockwise)

Preceding gene: 75674246

Following gene: 75674248

Centisome position: 1.62

GC content: 68.04

Gene sequence:

>726_bases
ATGCCGACCACTCCCACCAAAGCCATGGTGCTCGCCGCGGGCCTCGGCCTGCGCATGCGGCCGCTGACCGAACGGATGCC
CAAGCCCATGGTGCCCGTGGCCGGCAAGCCGCTGCTCGATCACGTGCTGGACAGGCTGGCGGACGCGGGCGTCAGCGAGG
CTGTCGTCAACGTGCGCTATCTGCCCGACCAGATCATCGATCACGTCGCTCACCGCGACCGGCCGAAGATCATCATCTCC
GACGAGCGCGACCAGGTGCTTGGCACCGGCGGTGCGGTGGTGAAGGCCCTGCCGCTGCTCGGCGACGCACCGTTCTTCCA
TCTCAATGCCGACACGATATGGATCGACGGCGCGCGTCCCAACCTGACGCGGCTGACCGAGGCCTTCGATCCCGCGAGCA
TGGACATTCTGCTGCTGATGGCGCCGACCGCGAGCAGCATCGGCTACAGCGGCAGCGGCGACTACGCCATGCTCCCCAAC
GGAGCGCTTCGCAGGCGACAAGAGAACCAGATCGTGCCGTTCGTCTATGCGGGAGCCGCCATCCTGTCGCCCGCGCTGTT
CGACGGCGCGCCGGCAGGCGAGTTTCCGCTGACCGCGATATTCGACCGGGCGGGTGAACGGGACCGGCTGTTCGGCCTGC
GGCTCGACGGCGTCTGGATGCATGTCGGAACACCCGATGCGGTCCAGGCCGCGGAGAAAGCCGTGCTCGCGAGCCTCGAC
GCCTGA

Upstream 100 bases:

>100_bases
CACACTAAAATCATAAAGTTGCTGGTGTGCCTTTGATTCCGAAGTTTGCATCAAAGGTTGCGACAAGGGTACGCGAACTT
CGGAATCGGGACTCCAGAAC

Downstream 100 bases:

>100_bases
CGGCTGCCTTTCAAATCTTCACCGCTTCCGGAGGATTCTCCGGCGAAGTGGATTCCACTTCGCCCGAAGGTTAAATCCCG
CCTATGTCATGATATGCCTG

Product: nucleotidyl transferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MPTTPTKAMVLAAGLGLRMRPLTERMPKPMVPVAGKPLLDHVLDRLADAGVSEAVVNVRYLPDQIIDHVAHRDRPKIIIS
DERDQVLGTGGAVVKALPLLGDAPFFHLNADTIWIDGARPNLTRLTEAFDPASMDILLLMAPTASSIGYSGSGDYAMLPN
GALRRRQENQIVPFVYAGAAILSPALFDGAPAGEFPLTAIFDRAGERDRLFGLRLDGVWMHVGTPDAVQAAEKAVLASLD
A

Sequences:

>Translated_241_residues
MPTTPTKAMVLAAGLGLRMRPLTERMPKPMVPVAGKPLLDHVLDRLADAGVSEAVVNVRYLPDQIIDHVAHRDRPKIIIS
DERDQVLGTGGAVVKALPLLGDAPFFHLNADTIWIDGARPNLTRLTEAFDPASMDILLLMAPTASSIGYSGSGDYAMLPN
GALRRRQENQIVPFVYAGAAILSPALFDGAPAGEFPLTAIFDRAGERDRLFGLRLDGVWMHVGTPDAVQAAEKAVLASLD
A
>Mature_240_residues
PTTPTKAMVLAAGLGLRMRPLTERMPKPMVPVAGKPLLDHVLDRLADAGVSEAVVNVRYLPDQIIDHVAHRDRPKIIISD
ERDQVLGTGGAVVKALPLLGDAPFFHLNADTIWIDGARPNLTRLTEAFDPASMDILLLMAPTASSIGYSGSGDYAMLPNG
ALRRRQENQIVPFVYAGAAILSPALFDGAPAGEFPLTAIFDRAGERDRLFGLRLDGVWMHVGTPDAVQAAEKAVLASLDA

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=240, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI11761621, Length=240, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=235, Percent_Identity=29.3617021276596, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=257, Percent_Identity=26.0700389105058, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355443, Length=254, Percent_Identity=25.5905511811024, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24644084, Length=254, Percent_Identity=25.5905511811024, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 25806; Mature: 25675

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTPTKAMVLAAGLGLRMRPLTERMPKPMVPVAGKPLLDHVLDRLADAGVSEAVVNVRY
CCCCCHHHHHHHHCCCCEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHEEEEE
LPDQIIDHVAHRDRPKIIISDERDQVLGTGGAVVKALPLLGDAPFFHLNADTIWIDGARP
CCHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHHCCCCCEEEECCCEEEEECCCC
NLTRLTEAFDPASMDILLLMAPTASSIGYSGSGDYAMLPNGALRRRQENQIVPFVYAGAA
CHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCEEEHHHH
ILSPALFDGAPAGEFPLTAIFDRAGERDRLFGLRLDGVWMHVGTPDAVQAAEKAVLASLD
HHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure 
PTTPTKAMVLAAGLGLRMRPLTERMPKPMVPVAGKPLLDHVLDRLADAGVSEAVVNVRY
CCCCHHHHHHHHCCCCEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHEEEEE
LPDQIIDHVAHRDRPKIIISDERDQVLGTGGAVVKALPLLGDAPFFHLNADTIWIDGARP
CCHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHHCCCCCEEEECCCEEEEECCCC
NLTRLTEAFDPASMDILLLMAPTASSIGYSGSGDYAMLPNGALRRRQENQIVPFVYAGAA
CHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCEEEHHHH
ILSPALFDGAPAGEFPLTAIFDRAGERDRLFGLRLDGVWMHVGTPDAVQAAEKAVLASLD
HHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]