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The map label for this gene is aphI [H]

Identifier: 73539515

GI number: 73539515

Start: 2523558

End: 2523749

Strand: Direct

Name: aphI [H]

Synonym: Reut_B5693

Alternate gene names: 73539515

Gene position: 2523558-2523749 (Clockwise)

Preceding gene: 73539514

Following gene: 73539518

Centisome position: 92.57

GC content: 54.69

Gene sequence:

>192_bases
ATGCCGTTTGCGCAGATCTACATGATTGAAGGCCGCACGGTGGAACAGAAGCGTGCCGTTATACAGAAAGTCACTTCTGC
CTTGGTGGAGGCGACGGGAGCGCCAAAGGAAAATGTACGCGTCTGGATCCACGATGTACCAAAGGAAAACTGGGGAATTG
CCGGCGAGTCGGCTAAAGACCTTGGGCGCTAG

Upstream 100 bases:

>100_bases
TGTCGCGTGCTCGAGTGATCGAGCACGGCGCTCAAGCTGGTCCGCGAGCATGGAGCGTGAGTGGAACCTGTTCACTAATG
GAATTTTTTGGAGGAATACG

Downstream 100 bases:

>100_bases
GTGCCGTGGCGAACGGGCGGAAGCCGATGTGGAGGCGACTATCGGATGTGCTCGGACAACGCTTCTTGAAGGGATCTCCG
AAGGCAAAAGCCGATGCCTG

Product: 4-oxalocrotonate tautomerase

Products: NA

Alternate protein names: 4-OT [H]

Number of amino acids: Translated: 63; Mature: 62

Protein sequence:

>63_residues
MPFAQIYMIEGRTVEQKRAVIQKVTSALVEATGAPKENVRVWIHDVPKENWGIAGESAKDLGR

Sequences:

>Translated_63_residues
MPFAQIYMIEGRTVEQKRAVIQKVTSALVEATGAPKENVRVWIHDVPKENWGIAGESAKDLGR
>Mature_62_residues
PFAQIYMIEGRTVEQKRAVIQKVTSALVEATGAPKENVRVWIHDVPKENWGIAGESAKDLGR

Specific function: Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate [H]

COG id: COG1942

COG function: function code R; Uncharacterized protein, 4-oxalocrotonate tautomerase homolog

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-oxalocrotonate tautomerase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004370
- InterPro:   IPR018191
- InterPro:   IPR014347 [H]

Pfam domain/function: PF01361 Tautomerase [H]

EC number: NA

Molecular weight: Translated: 7021; Mature: 6890

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFAQIYMIEGRTVEQKRAVIQKVTSALVEATGAPKENVRVWIHDVPKENWGIAGESAKD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
LGR
CCC
>Mature Secondary Structure 
PFAQIYMIEGRTVEQKRAVIQKVTSALVEATGAPKENVRVWIHDVPKENWGIAGESAKD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
LGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10878134 [H]