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The map label for this gene is sbcD [H]

Identifier: 73539404

GI number: 73539404

Start: 2398546

End: 2399691

Strand: Direct

Name: sbcD [H]

Synonym: Reut_B5582

Alternate gene names: 73539404

Gene position: 2398546-2399691 (Clockwise)

Preceding gene: 73539403

Following gene: 73539405

Centisome position: 87.98

GC content: 67.71

Gene sequence:

>1146_bases
TTGCGTTTCCTGCATACCGCCGACTGGCATCTCGGGCGGCTCTTTCATGCCCGCAGCCTGATTGAAGACCAGGCCTACAT
CCTCGACCAGTTTGTCGAACTGGTGCGCACCGAGCGGCCCGACGCCGTGCTGATCGCCGGCGATGTCTATGACCGTGCCG
TGCCGCCGCCGGAAGCGGTGGCGCTGCTCGATGACGTGCTGGCACGGATCATCCTGGACGTGGGCGTTCCTGTCGTGCTG
ATCGCCGGCAACCACGACAGCGCGCAGCGGCTGGAATTCGGCGCCCGGCTGATGCAGTCGCAGGGCCTGCACGTTGCCGG
CTGCACGTCCGCCACACCGGCCTGCGTGTCGTTGCATGACGCGCATGGCGAAGTGCGACTCTATGCGCTGCCGTATGCGG
AGCCGGCCGTCGTGCGCGACGCGCTCGGTGCCGAACTGCCCACCCATGAGGCGGCGTTGTGCGCGCAGCTCGACGCGATT
CGTGCGAGCCATCCGGCCGGTATGCGCTCCGTGGTGGTCGGCCATGCGTTCGTGGTCGGCGGCGCGGTCAGCGAATCCGA
GCGGCCGTTATCGGTGGGCGGCAGCGGCGCTGTTGCCGCGCAAGCGTTCCAAGGGTTCGACCTTGTTGCGCTGGGCCACT
TGCACCGGCCCCAGACCCTCGACGGGCGGGTGCATTACGCGGGCTCGCTGCTCAAGTATTCGTTGTCGGAATGCGGTCAC
GACAAATCCGTGTCGATGATCGACCTTGGCGCTGACGGCAGCGTGCAAATCACGCCCATGCCCCTGCGGCCGCTACGCGA
CCTGCGTATCGTGGAAGGCGAACTCAGCGAGCTGATCGCCGCTGCCGCCGATGACGCGCGACGTGATGACTATGTCTATG
CACGCCTGACGGACACCGGCGCGCTGCTGGACCCGATGGCGAAGCTGCGGCAGGCTTATCCCAACGCGCTCGCGGTCGAG
CGCACCGTGCTGGCACGCGGCGGTGTAGCCGGCGATGCGGCGCGGCGCCTGCGCGAACAAAGCACGGGCGAGCTCTTCGC
GAGCTTCTTCCGCGAAGTGGTCGATGCGGAACTCGATGATGGCCAGCGTGGCGCGCTGGAACAGGTGCTGGCCGGCATGG
CCGCGGCACAGCGGGAGTCCGCATGA

Upstream 100 bases:

>100_bases
AGGCGGCACCTGTGCGCCGCGCGCACAGTTATGATGCCGGCTGTACCGCGTGCCGCCGTGACGGACACGCCACCCCCACA
CCGCAGACCAGGTATCTACA

Downstream 100 bases:

>100_bases
GACCCCTGCACCTGACAATGCAGGCCTTCGGGCCGTTTTCCGGCACTGAGGCAATCGATTTCACGCGCCTGGGCGAGCAG
GCCTTCTTCCTGATTCACGG

Product: exodeoxyribonuclease I subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRFLHTADWHLGRLFHARSLIEDQAYILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAVALLDDVLARIILDVGVPVVL
IAGNHDSAQRLEFGARLMQSQGLHVAGCTSATPACVSLHDAHGEVRLYALPYAEPAVVRDALGAELPTHEAALCAQLDAI
RASHPAGMRSVVVGHAFVVGGAVSESERPLSVGGSGAVAAQAFQGFDLVALGHLHRPQTLDGRVHYAGSLLKYSLSECGH
DKSVSMIDLGADGSVQITPMPLRPLRDLRIVEGELSELIAAAADDARRDDYVYARLTDTGALLDPMAKLRQAYPNALAVE
RTVLARGGVAGDAARRLREQSTGELFASFFREVVDAELDDGQRGALEQVLAGMAAAQRESA

Sequences:

>Translated_381_residues
MRFLHTADWHLGRLFHARSLIEDQAYILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAVALLDDVLARIILDVGVPVVL
IAGNHDSAQRLEFGARLMQSQGLHVAGCTSATPACVSLHDAHGEVRLYALPYAEPAVVRDALGAELPTHEAALCAQLDAI
RASHPAGMRSVVVGHAFVVGGAVSESERPLSVGGSGAVAAQAFQGFDLVALGHLHRPQTLDGRVHYAGSLLKYSLSECGH
DKSVSMIDLGADGSVQITPMPLRPLRDLRIVEGELSELIAAAADDARRDDYVYARLTDTGALLDPMAKLRQAYPNALAVE
RTVLARGGVAGDAARRLREQSTGELFASFFREVVDAELDDGQRGALEQVLAGMAAAQRESA
>Mature_381_residues
MRFLHTADWHLGRLFHARSLIEDQAYILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAVALLDDVLARIILDVGVPVVL
IAGNHDSAQRLEFGARLMQSQGLHVAGCTSATPACVSLHDAHGEVRLYALPYAEPAVVRDALGAELPTHEAALCAQLDAI
RASHPAGMRSVVVGHAFVVGGAVSESERPLSVGGSGAVAAQAFQGFDLVALGHLHRPQTLDGRVHYAGSLLKYSLSECGH
DKSVSMIDLGADGSVQITPMPLRPLRDLRIVEGELSELIAAAADDARRDDYVYARLTDTGALLDPMAKLRQAYPNALAVE
RTVLARGGVAGDAARRLREQSTGELFASFFREVVDAELDDGQRGALEQVLAGMAAAQRESA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=399, Percent_Identity=27.3182957393484, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 40694; Mature: 40694

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTADWHLGRLFHARSLIEDQAYILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
ALLDDVLARIILDVGVPVVLIAGNHDSAQRLEFGARLMQSQGLHVAGCTSATPACVSLHD
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHEEEEC
AHGEVRLYALPYAEPAVVRDALGAELPTHEAALCAQLDAIRASHPAGMRSVVVGHAFVVG
CCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEC
GAVSESERPLSVGGSGAVAAQAFQGFDLVALGHLHRPQTLDGRVHYAGSLLKYSLSECGH
CCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHHCCC
DKSVSMIDLGADGSVQITPMPLRPLRDLRIVEGELSELIAAAADDARRDDYVYARLTDTG
CCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
ALLDPMAKLRQAYPNALAVERTVLARGGVAGDAARRLREQSTGELFASFFREVVDAELDD
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GQRGALEQVLAGMAAAQRESA
CCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFLHTADWHLGRLFHARSLIEDQAYILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
ALLDDVLARIILDVGVPVVLIAGNHDSAQRLEFGARLMQSQGLHVAGCTSATPACVSLHD
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHEEEEC
AHGEVRLYALPYAEPAVVRDALGAELPTHEAALCAQLDAIRASHPAGMRSVVVGHAFVVG
CCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEC
GAVSESERPLSVGGSGAVAAQAFQGFDLVALGHLHRPQTLDGRVHYAGSLLKYSLSECGH
CCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHHCCC
DKSVSMIDLGADGSVQITPMPLRPLRDLRIVEGELSELIAAAADDARRDDYVYARLTDTG
CCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
ALLDPMAKLRQAYPNALAVERTVLARGGVAGDAARRLREQSTGELFASFFREVVDAELDD
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GQRGALEQVLAGMAAAQRESA
CCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA