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The map label for this gene is acoB [H]

Identifier: 73539373

GI number: 73539373

Start: 2357683

End: 2358699

Strand: Direct

Name: acoB [H]

Synonym: Reut_B5551

Alternate gene names: 73539373

Gene position: 2357683-2358699 (Clockwise)

Preceding gene: 73539372

Following gene: 73539374

Centisome position: 86.48

GC content: 63.91

Gene sequence:

>1017_bases
ATGGCTCGCAAACTGAGCATCAAGCTGGCGATCAACGAGGCGATCGACCAGGAAATGACGCGGGATCCCAGCGTGATCAT
GCTGGGGGAAGACATTGTCGGCGGCGCCGGCGCGGACGGTGAAAAGGACGCATGGGGCGGTGTGCTTGGCGTGACCAAGG
GGTTGTACGCCAAGCATGGCGACCGCTTGCTCGATACGCCACTGTCGGAGTCGGCCTACGTGGGGGCGGCCATCGGCGCC
GCGGCCTGCGGCATGCGACCGATCGCCGAACTGATGTTCATCGACTTCATGGGCGTGTGCTTCGACCAGATCTTCAATCA
GGCGGCCAAGTTCCGCTACATGTTCGGTGGCAAGGCCGAAACGCCGGTGGTGATCCGCGCAATGGTAGGCGCGGGCTTTC
GCGCGGCCGCGCAGCACAGCCAGATGCTGACGCCGCTGTTCACGCACATTCCCGGCCTCAAGGTGGTGTGCCCCTCGACG
CCGTACGACACCAAGGGCCTGCTGATCCAGGCCATCCGCGACAACGATCCGGTGATCTTCTGCGAGCACAAGAACCTGTA
CGGGCTTGAAGGCGACGTGCCGGAGGGCGCTTACACGATTCCGTTCGGCGAGGCCAATATCGCACGCGACGGCAAGGACG
TGACCATCGTCGCTTACGGGCTGATGGTGCATCGCGCGCTGGACGCGGCGGCGACGCTGGCGAAGGAAGGCATCGAAGCG
GAAGTGATTGACCTGCGCACGCTGTCGCCGCTCGACATGGATACGGTGCTCGAGTCGGTCGAGAACACCGGACGGCTTGT
CGTCGTGGACGAAGCTAGCCCGCGCTGCAATATCGCGACTGATATCTCGGCGCAGGTTGCGCAGCAGGCATTCGGTGCGC
TGAAGGCCGGTATCGAGATGGTGTGCCCGCCGCACACGCCGGTTCCGTTTTCGCCGACGCTCGAAGACCTGTATATCCCC
AGCGCCGCGCACGTCGTGGAAGCGGTGCGCAAGACCATGAAGGGAGGCAAGCACTGA

Upstream 100 bases:

>100_bases
GCCGCGCCATTGCCTGGTCCGGAGGATCTGCTGACTGATGTCTACGTGAGCTACTGAATCGCCAACCCGACACTGCTATC
CACATTTCAGGAGACAGACC

Downstream 100 bases:

>100_bases
TGGCCGCAGCAATTACCCCGATCGTGATGCCCAAGTGGGGCCTGTCGATGAAGGAAGGCACTATCAACGAGTGGCTCGTC
GACGAGGGCGCCCAAATATC

Product: transketolase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGA
AACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPST
PYDTKGLLIQAIRDNDPVIFCEHKNLYGLEGDVPEGAYTIPFGEANIARDGKDVTIVAYGLMVHRALDAAATLAKEGIEA
EVIDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIP
SAAHVVEAVRKTMKGGKH

Sequences:

>Translated_338_residues
MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGA
AACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPST
PYDTKGLLIQAIRDNDPVIFCEHKNLYGLEGDVPEGAYTIPFGEANIARDGKDVTIVAYGLMVHRALDAAATLAKEGIEA
EVIDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIP
SAAHVVEAVRKTMKGGKH
>Mature_337_residues
ARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGAA
ACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTP
YDTKGLLIQAIRDNDPVIFCEHKNLYGLEGDVPEGAYTIPFGEANIARDGKDVTIVAYGLMVHRALDAAATLAKEGIEAE
VIDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIPS
AAHVVEAVRKTMKGGKH

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=339, Percent_Identity=39.5280235988201, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI291084858, Length=339, Percent_Identity=37.7581120943953, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI4557353, Length=337, Percent_Identity=39.4658753709199, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI34101272, Length=337, Percent_Identity=39.4658753709199, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI225637463, Length=293, Percent_Identity=26.6211604095563, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI225637459, Length=293, Percent_Identity=26.6211604095563, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI225637461, Length=293, Percent_Identity=26.6211604095563, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=339, Percent_Identity=41.2979351032448, Blast_Score=254, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17506935, Length=336, Percent_Identity=41.6666666666667, Blast_Score=211, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=338, Percent_Identity=36.6863905325444, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358145, Length=342, Percent_Identity=39.4736842105263, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24650940, Length=342, Percent_Identity=39.4736842105263, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI160714832, Length=332, Percent_Identity=35.2409638554217, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI160714828, Length=332, Percent_Identity=35.2409638554217, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24650943, Length=102, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650945, Length=102, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36027; Mature: 35896

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHG
CCCEEEEEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
DRLLDTPLSESAYVGAAIGAAACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAE
CHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTPYDTKGLLIQAIRDNDPVIF
CCEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEEE
CEHKNLYGLEGDVPEGAYTIPFGEANIARDGKDVTIVAYGLMVHRALDAAATLAKEGIEA
EECCCEECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
EVIDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEM
EEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCEE
VCPPHTPVPFSPTLEDLYIPSAAHVVEAVRKTMKGGKH
ECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHG
CCEEEEEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
DRLLDTPLSESAYVGAAIGAAACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAE
CHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTPYDTKGLLIQAIRDNDPVIF
CCEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEEE
CEHKNLYGLEGDVPEGAYTIPFGEANIARDGKDVTIVAYGLMVHRALDAAATLAKEGIEA
EECCCEECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
EVIDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEM
EEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCEE
VCPPHTPVPFSPTLEDLYIPSAAHVVEAVRKTMKGGKH
ECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]