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The map label for this gene is acoA [H]

Identifier: 73539372

GI number: 73539372

Start: 2356635

End: 2357639

Strand: Direct

Name: acoA [H]

Synonym: Reut_B5550

Alternate gene names: 73539372

Gene position: 2356635-2357639 (Clockwise)

Preceding gene: 73539371

Following gene: 73539373

Centisome position: 86.45

GC content: 65.87

Gene sequence:

>1005_bases
ATGAGCGCCAGTGCCATGCAAGCCGGCCCAAGCGGGCTGCCACTCGACAAGGAGACCCTGCTGACGGTGTATCGGAAGAT
GCGCACCATCCGCGAGTTCGAAGAACGCCTGCACGTGGACTTCGGCCGCGGCGACATTCCCGGTTTCGTGCATCTGTACG
CGGGCGAGGAGGCCGCGGGCGTCGGCATCCTGCACCACCTGCACGATGGCGACCGCATCGCCAGTACGCACCGCGGTCAC
GGCCACTGCATTGCCAAGGGCGTCGACCCGGTGGCGATGATGAAGGAGATCTACGGCAAGAAGGGCGGCTCTTGCAATGG
CAAGGGCGGGTCGATGCATATCGCCGACCTGTCCAAGGGCATGATGGGCGCAAACGGCATTCTGGGCGCCGGCGCGCCGC
TGATCTGCGGCGCCGCGCTGGCCGCGAAGTTCCGCGGCAAGGGCGAGGTCGGCATTACGTTCAGCGGCGACGGCGCATCG
AACCAGGGCACGTTCCTCGAAAGCCTGAACCTCGCCGCCGTGTGGAACCTGCCGGTGATCTTCGTGATCGAGAACAACGG
CTATGCGGAATCGACCTCGCGCGATTACGGCACCGCGGTGGACAGCTACGTGGATCGCGCGGCCGGCTTCGGCATTCCCG
GCGTGACGGTGGACGGTACCGATTTCTTCGCGGTGCATGAGGCAGCCGGCGAAGTCATCCGCCGCGCGCGTGAAGGCGGT
GGGCCGTCGCTGCTCGAATGCAAGATGGTCCGCTTCTACGGGCACTTCGAAGGCGACGCGCAAACCTATCGCGCCGCGGG
CGAACTCGACGACATCCGCGCCAACAAGGACTGCCTGAAGCTGTTCTCGCGCGCGGTGACGCAAGCGGGCGTGGTCTCGC
GCGAGGAACTCGAGTCGATCGACCGCGAAGTCGCGACGCTGATCGAGCACGCCGTGCAGGAGGCCAAGGCCGCGCCATTG
CCTGGTCCGGAGGATCTGCTGACTGATGTCTACGTGAGCTACTGA

Upstream 100 bases:

>100_bases
CATGCGCCATGCCGCGCGCGAGCGGCTGATGCGCAGCAGTCCAGCCGCCATTCTCGCTAATCACTGAGCAAGCCGTACCA
CCCCAACCAAGGAGACAACC

Downstream 100 bases:

>100_bases
ATCGCCAACCCGACACTGCTATCCACATTTCAGGAGACAGACCATGGCTCGCAAACTGAGCATCAAGCTGGCGATCAACG
AGGCGATCGACCAGGAAATG

Product: pyruvate dehydrogenase (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSASAMQAGPSGLPLDKETLLTVYRKMRTIREFEERLHVDFGRGDIPGFVHLYAGEEAAGVGILHHLHDGDRIASTHRGH
GHCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFSGDGAS
NQGTFLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGG
GPSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFSRAVTQAGVVSREELESIDREVATLIEHAVQEAKAAPL
PGPEDLLTDVYVSY

Sequences:

>Translated_334_residues
MSASAMQAGPSGLPLDKETLLTVYRKMRTIREFEERLHVDFGRGDIPGFVHLYAGEEAAGVGILHHLHDGDRIASTHRGH
GHCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFSGDGAS
NQGTFLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGG
GPSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFSRAVTQAGVVSREELESIDREVATLIEHAVQEAKAAPL
PGPEDLLTDVYVSY
>Mature_333_residues
SASAMQAGPSGLPLDKETLLTVYRKMRTIREFEERLHVDFGRGDIPGFVHLYAGEEAAGVGILHHLHDGDRIASTHRGHG
HCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFSGDGASN
QGTFLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGGG
PSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFSRAVTQAGVVSREELESIDREVATLIEHAVQEAKAAPLP
GPEDLLTDVYVSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=325, Percent_Identity=36.6153846153846, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI291084742, Length=325, Percent_Identity=39.3846153846154, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI4505685, Length=325, Percent_Identity=39.3846153846154, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI291084744, Length=332, Percent_Identity=38.5542168674699, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI291084757, Length=325, Percent_Identity=34.1538461538462, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI11386135, Length=326, Percent_Identity=28.2208588957055, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI258645172, Length=326, Percent_Identity=28.2208588957055, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536047, Length=328, Percent_Identity=36.280487804878, Blast_Score=217, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI32564172, Length=328, Percent_Identity=36.280487804878, Blast_Score=217, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI86563357, Length=321, Percent_Identity=26.791277258567, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI86563355, Length=321, Percent_Identity=26.791277258567, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=36.0501567398119, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24639744, Length=328, Percent_Identity=36.890243902439, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI28571106, Length=328, Percent_Identity=36.890243902439, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24639740, Length=328, Percent_Identity=36.890243902439, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=37.5816993464052, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24639748, Length=328, Percent_Identity=34.1463414634146, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21355903, Length=312, Percent_Identity=26.6025641025641, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35443; Mature: 35312

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAMQAGPSGLPLDKETLLTVYRKMRTIREFEERLHVDFGRGDIPGFVHLYAGEEAAG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
VGILHHLHDGDRIASTHRGHGHCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKG
HHHHHHHCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCC
MMGANGILGAGAPLICGAALAAKFRGKGEVGITFSGDGASNQGTFLESLNLAAVWNLPVI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHCCEEEEECCCEE
FVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGG
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC
GPSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFSRAVTQAGVVSREELESI
CCCHHHHHEEHHHCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
DREVATLIEHAVQEAKAAPLPGPEDLLTDVYVSY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SASAMQAGPSGLPLDKETLLTVYRKMRTIREFEERLHVDFGRGDIPGFVHLYAGEEAAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
VGILHHLHDGDRIASTHRGHGHCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKG
HHHHHHHCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCC
MMGANGILGAGAPLICGAALAAKFRGKGEVGITFSGDGASNQGTFLESLNLAAVWNLPVI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHCCEEEEECCCEE
FVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGG
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC
GPSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFSRAVTQAGVVSREELESI
CCCHHHHHEEHHHCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
DREVATLIEHAVQEAKAAPLPGPEDLLTDVYVSY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]