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The map label for this gene is kgtP [H]

Identifier: 73539317

GI number: 73539317

Start: 2292034

End: 2293347

Strand: Reverse

Name: kgtP [H]

Synonym: Reut_B5495

Alternate gene names: 73539317

Gene position: 2293347-2292034 (Counterclockwise)

Preceding gene: 73539319

Following gene: 73539316

Centisome position: 84.12

GC content: 64.23

Gene sequence:

>1314_bases
ATGACCGATCTCACAGCCAACTCCAACCAGGCCACCGCACCATCGGCTGAAGAGGTGCGCAAGCGCGTCTTCGCCATCGT
TGCCGCCTCTTCCGGCAATCTCGTCGAATGGTTCGACTTCTATGTCTACGCGTTCTGCGCCATCTACTTCGCGCCGTCGT
TCTTTCCCAAGGCCGATCCGACAGCCCAGCTGCTGAACACAGCCGGCGTGTTCGCCGCAGGCTTCCTGATGCGGCCGATC
GGCGGCTGGCTGTTCGGCACCATCGCCGACCGCCACGGCCGCAAGAACTCGATGCTGATCTCCGTGCTGATGATGTGCTT
CGGCTCGCTGCTGATCGCCAGCCTGCCGACGTACAGCAGCATCGGCAACTGGGCGCCGGCACTGCTGCTGCTGGCACGTC
TGATCCAGGGCCTGTCGGTCGGAGGCGAATACGGCACCACCGCCACCTACATGAGCGAAGTGGCGCTGCGCGGTCGACGC
GGCTTCTTCTCTTCGTTCCAGTACGTCACGCTGATCGGCGGCCAGTTGCTGGCTGTGCTGGTCGTGGTGATCCTGCAGCA
GCTGCTTGATGAAGCCGAGCTCAAGGCCTGGGGCTGGCGCATTCCGTTCGTGGTCGGCGCCATTACCGCCGTCGTGGCGC
TGTTCCTGCGCCGCACACTGCACGAGACCTCGTCCGCGGCAACGCGCGGCAGCAAGGAAGCCGGCAGCGTCGCGGAGATC
TTCCGCAACCACAAGGCCGCGTTCTTCACCGTGCTCGGCTATACCGCCGGCGGCTCGCTGATTTTCTACACCTTCACCAC
GTACATGCAGAAGTACCTGGTCAACACCGCGGGCATGTCGATCAAGACCGCCAGCTATGTGATGACGGGATGCCTGTTCC
TGTACATGTGCATACAGCCCGTGTTCGGCGCGTTGTCGGACCGCATCGGCCGGCGCAACAACATGCTGCTGTTCGGCGCG
CTGGGCGCACTCGCCACGGTGCCGATCCTGACCGCGCTGCGCACGGTAAGCAGCCCCGAACTGGCGTTCGTGCTGATTGC
CGTGGCGCTCGCCATCGTCAGCTTCTATACGTCGATCAGCGGCATCGTGAAGGCGGAGATGTTCCCGGCCGAAGTGCGCG
CGCTCGGCGTCGGGCTGGCGTATGCCATCGCCAACGCGGCGTTCGGCGGCACGGCCGAATACGTGGCGCTCGGGCTCAAG
ACCATGGGCCACGAATCCGCGTTCTACTGGTATGTCACCGTGATGATGGTGATCGCGTTCCTGGTCAGCCTGCGCCTGCC
GCGCCAGGCGAAGTACCTGCGTCACGAGCACTGA

Upstream 100 bases:

>100_bases
ATCCCGGAACCCGGCGGCGTTCCAACCGGCGCGAGCCGCCCTGCGGCCAAGCTGACGCTGGCCAGCCGCCGGGCATCGCC
CCTGACTGCAACACACAACA

Downstream 100 bases:

>100_bases
GCCGGCTTTGCGCGCCATGCCGGCGGCCCGGCGAGGGGGTGCTGGCGGCGGCGCGTGGCAATATACGGGGGCGGACGCTG
CCGGCGGCAGCCCGCGCCCT

Product: citrate-proton symport

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTDLTANSNQATAPSAEEVRKRVFAIVAASSGNLVEWFDFYVYAFCAIYFAPSFFPKADPTAQLLNTAGVFAAGFLMRPI
GGWLFGTIADRHGRKNSMLISVLMMCFGSLLIASLPTYSSIGNWAPALLLLARLIQGLSVGGEYGTTATYMSEVALRGRR
GFFSSFQYVTLIGGQLLAVLVVVILQQLLDEAELKAWGWRIPFVVGAITAVVALFLRRTLHETSSAATRGSKEAGSVAEI
FRNHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMSIKTASYVMTGCLFLYMCIQPVFGALSDRIGRRNNMLLFGA
LGALATVPILTALRTVSSPELAFVLIAVALAIVSFYTSISGIVKAEMFPAEVRALGVGLAYAIANAAFGGTAEYVALGLK
TMGHESAFYWYVTVMMVIAFLVSLRLPRQAKYLRHEH

Sequences:

>Translated_437_residues
MTDLTANSNQATAPSAEEVRKRVFAIVAASSGNLVEWFDFYVYAFCAIYFAPSFFPKADPTAQLLNTAGVFAAGFLMRPI
GGWLFGTIADRHGRKNSMLISVLMMCFGSLLIASLPTYSSIGNWAPALLLLARLIQGLSVGGEYGTTATYMSEVALRGRR
GFFSSFQYVTLIGGQLLAVLVVVILQQLLDEAELKAWGWRIPFVVGAITAVVALFLRRTLHETSSAATRGSKEAGSVAEI
FRNHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMSIKTASYVMTGCLFLYMCIQPVFGALSDRIGRRNNMLLFGA
LGALATVPILTALRTVSSPELAFVLIAVALAIVSFYTSISGIVKAEMFPAEVRALGVGLAYAIANAAFGGTAEYVALGLK
TMGHESAFYWYVTVMMVIAFLVSLRLPRQAKYLRHEH
>Mature_436_residues
TDLTANSNQATAPSAEEVRKRVFAIVAASSGNLVEWFDFYVYAFCAIYFAPSFFPKADPTAQLLNTAGVFAAGFLMRPIG
GWLFGTIADRHGRKNSMLISVLMMCFGSLLIASLPTYSSIGNWAPALLLLARLIQGLSVGGEYGTTATYMSEVALRGRRG
FFSSFQYVTLIGGQLLAVLVVVILQQLLDEAELKAWGWRIPFVVGAITAVVALFLRRTLHETSSAATRGSKEAGSVAEIF
RNHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMSIKTASYVMTGCLFLYMCIQPVFGALSDRIGRRNNMLLFGAL
GALATVPILTALRTVSSPELAFVLIAVALAIVSFYTSISGIVKAEMFPAEVRALGVGLAYAIANAAFGGTAEYVALGLKT
MGHESAFYWYVTVMMVIAFLVSLRLPRQAKYLRHEH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=433, Percent_Identity=68.8221709006928, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=30.2816901408451, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788292, Length=385, Percent_Identity=31.4285714285714, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=31.0843373493976, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082231, Length=301, Percent_Identity=23.9202657807309, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082404, Length=402, Percent_Identity=26.3681592039801, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47375; Mature: 47244

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTANSNQATAPSAEEVRKRVFAIVAASSGNLVEWFDFYVYAFCAIYFAPSFFPKADP
CCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TAQLLNTAGVFAAGFLMRPIGGWLFGTIADRHGRKNSMLISVLMMCFGSLLIASLPTYSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
IGNWAPALLLLARLIQGLSVGGEYGTTATYMSEVALRGRRGFFSSFQYVTLIGGQLLAVL
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VVVILQQLLDEAELKAWGWRIPFVVGAITAVVALFLRRTLHETSSAATRGSKEAGSVAEI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FRNHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMSIKTASYVMTGCLFLYMCIQP
HHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VFGALSDRIGRRNNMLLFGALGALATVPILTALRTVSSPELAFVLIAVALAIVSFYTSIS
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GIVKAEMFPAEVRALGVGLAYAIANAAFGGTAEYVALGLKTMGHESAFYWYVTVMMVIAF
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVSLRLPRQAKYLRHEH
HHHHCCCHHHHHHCCCC
>Mature Secondary Structure 
TDLTANSNQATAPSAEEVRKRVFAIVAASSGNLVEWFDFYVYAFCAIYFAPSFFPKADP
CCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC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HHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]