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The map label for this gene is eutB [H]

Identifier: 73539312

GI number: 73539312

Start: 2287840

End: 2289222

Strand: Direct

Name: eutB [H]

Synonym: Reut_B5490

Alternate gene names: 73539312

Gene position: 2287840-2289222 (Clockwise)

Preceding gene: 73539310

Following gene: 73539313

Centisome position: 83.92

GC content: 65.87

Gene sequence:

>1383_bases
ATGGCATTCACCCACACCGTCGGACAGCACCGTCATGTCTTTGCCGACCTGCGCACGCTGCTGGCCAAGGCCAGCCCCGC
ACGTTCGGGCGACTATCTTGCCGGTATCGCCGCCGCCAGCGAGGAAGAGCGCATGGCGGCGCGCATGGCGCTCGCCGCGG
TGCCGCTGACGCAGTTCCTGTCTGAAGCGCTCGTGCCCTATGAGGACGATGAGGTCACCCGCCTGATCGTGGATAGTCAC
GATGCTGTCGCGTTTGCCGAGATCGCATCGCTGACAGTTGGGGATTTCCGCAACTGGTTGCTGCTGCACGAGACCGACAG
CACCACGCTTGCAACTGTAGCGCGCGGCATCACGCCCGAGATGGCTGCCGCAGTCAGCAAGCTCATGCGCAACCAGGACC
TCATCGCCGTCGCGCGCAAATGCCACGTGGTCACGCGCTTTCGCAGCACCGTGGGCCTGCCGGGCCGTCTCTCGGTGCGG
CTGCAGCCGAACCATCCCACGGATGATCCAAAGGGCATCGCCGCGTCGATCATCGACGGACTGATGTATGGCTGCGGCGA
TGCGACCATCGGTGTCAATCCGGTGTCGGACAGCCTCGGTGCGATCGTGTCGCTGCTGCGCATGATCGACGACCTGCGCT
GCCGCTACGACATTCCCACGCAGTCGTGCGTGCTCACGCACGTCACTCACACGCTGCGCGCGATGGAGCAGGGCGCGCCC
GTGGATCTCGTGTTCCAGTCGGTAGCGGGCAGCGAGCGCGCCAATGCGGCGTTCGGCATCAGCCTGTCGCTGCTGGCCGA
AGCGCACGACGCCGCGCAGGCGCTCGCGCGCGGCACCGTTGGCGATAACGTGATGTATTTCGAGACCGGGCAGGGCAGCG
CGCTGTCGGCCAACGCGCACCACGGCGTGGACCAGCAGACCATGGAGGCACGCGCGTATGCGGTGGCGCGGCGATTCTCG
CCGCTGCTCGTCAATACCGTGGTCGGCTTCATCGGCCCCGAGTACCTGTATGACGGCAAGCAGATCATCCGCGCCGGGCT
CGAAGACCATTTCTGCGGCAAGCTGCTTGGCGTGCCGATGGGTTGCGATGTCTGCTACACCAACCACGCCGAGGCCGACC
AGGACGACATGGACACGCTGCTGACGCTGTTCGGCGTGGCGGGCATCAACTTCATTATGGGCGTGCCCGGCGCCGACGAT
ATCATGCTGAACTACCAGAGCACGTCGTTCCATGACGCGCTGTACCTGCGCGAAGTGCTGGGGCTGCGCCCGGCACCCGA
ATTCGAGGCGTGGCTGCAGCGCATGGGCATCGCCGATGGCAGCGGCCGCTTGCTGGAACCGGCTGAGCGGCAGCCGCTGC
TCGAACTGGCGCACACGCTGTAA

Upstream 100 bases:

>100_bases
GTAACCTGAAGGTCTATAGTTACGATACCGGCGCAATATCGCCCGGTCCCGATGGCGCATGCGGCATGCCATCGCGTGGT
TTGCCCCGGAGCCCCCACTC

Downstream 100 bases:

>100_bases
GCCCCGCCATGACCAAGCCACCAGCGACCGTCACGCAGCAGAATCCCTGGCAACAGTTGCGCCAGTTCACGCGCGCACGC
ATTGCATTGGGCCGTGCAGG

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAFTHTVGQHRHVFADLRTLLAKASPARSGDYLAGIAAASEEERMAARMALAAVPLTQFLSEALVPYEDDEVTRLIVDSH
DAVAFAEIASLTVGDFRNWLLLHETDSTTLATVARGITPEMAAAVSKLMRNQDLIAVARKCHVVTRFRSTVGLPGRLSVR
LQPNHPTDDPKGIAASIIDGLMYGCGDATIGVNPVSDSLGAIVSLLRMIDDLRCRYDIPTQSCVLTHVTHTLRAMEQGAP
VDLVFQSVAGSERANAAFGISLSLLAEAHDAAQALARGTVGDNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFS
PLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDTLLTLFGVAGINFIMGVPGADD
IMLNYQSTSFHDALYLREVLGLRPAPEFEAWLQRMGIADGSGRLLEPAERQPLLELAHTL

Sequences:

>Translated_460_residues
MAFTHTVGQHRHVFADLRTLLAKASPARSGDYLAGIAAASEEERMAARMALAAVPLTQFLSEALVPYEDDEVTRLIVDSH
DAVAFAEIASLTVGDFRNWLLLHETDSTTLATVARGITPEMAAAVSKLMRNQDLIAVARKCHVVTRFRSTVGLPGRLSVR
LQPNHPTDDPKGIAASIIDGLMYGCGDATIGVNPVSDSLGAIVSLLRMIDDLRCRYDIPTQSCVLTHVTHTLRAMEQGAP
VDLVFQSVAGSERANAAFGISLSLLAEAHDAAQALARGTVGDNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFS
PLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDTLLTLFGVAGINFIMGVPGADD
IMLNYQSTSFHDALYLREVLGLRPAPEFEAWLQRMGIADGSGRLLEPAERQPLLELAHTL
>Mature_459_residues
AFTHTVGQHRHVFADLRTLLAKASPARSGDYLAGIAAASEEERMAARMALAAVPLTQFLSEALVPYEDDEVTRLIVDSHD
AVAFAEIASLTVGDFRNWLLLHETDSTTLATVARGITPEMAAAVSKLMRNQDLIAVARKCHVVTRFRSTVGLPGRLSVRL
QPNHPTDDPKGIAASIIDGLMYGCGDATIGVNPVSDSLGAIVSLLRMIDDLRCRYDIPTQSCVLTHVTHTLRAMEQGAPV
DLVFQSVAGSERANAAFGISLSLLAEAHDAAQALARGTVGDNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFSP
LLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDTLLTLFGVAGINFIMGVPGADDI
MLNYQSTSFHDALYLREVLGLRPAPEFEAWLQRMGIADGSGRLLEPAERQPLLELAHTL

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=448, Percent_Identity=49.5535714285714, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 49583; Mature: 49452

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTHTVGQHRHVFADLRTLLAKASPARSGDYLAGIAAASEEERMAARMALAAVPLTQFL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHH
SEALVPYEDDEVTRLIVDSHDAVAFAEIASLTVGDFRNWLLLHETDSTTLATVARGITPE
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCHH
MAAAVSKLMRNQDLIAVARKCHVVTRFRSTVGLPGRLSVRLQPNHPTDDPKGIAASIIDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
LMYGCGDATIGVNPVSDSLGAIVSLLRMIDDLRCRYDIPTQSCVLTHVTHTLRAMEQGAP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
VDLVFQSVAGSERANAAFGISLSLLAEAHDAAQALARGTVGDNVMYFETGQGSALSANAH
HHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCC
HGVDQQTMEARAYAVARRFSPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPM
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCC
GCDVCYTNHAEADQDDMDTLLTLFGVAGINFIMGVPGADDIMLNYQSTSFHDALYLREVL
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
GLRPAPEFEAWLQRMGIADGSGRLLEPAERQPLLELAHTL
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHCCHHHHHHHCC
>Mature Secondary Structure 
AFTHTVGQHRHVFADLRTLLAKASPARSGDYLAGIAAASEEERMAARMALAAVPLTQFL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHH
SEALVPYEDDEVTRLIVDSHDAVAFAEIASLTVGDFRNWLLLHETDSTTLATVARGITPE
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCHH
MAAAVSKLMRNQDLIAVARKCHVVTRFRSTVGLPGRLSVRLQPNHPTDDPKGIAASIIDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
LMYGCGDATIGVNPVSDSLGAIVSLLRMIDDLRCRYDIPTQSCVLTHVTHTLRAMEQGAP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
VDLVFQSVAGSERANAAFGISLSLLAEAHDAAQALARGTVGDNVMYFETGQGSALSANAH
HHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCC
HGVDQQTMEARAYAVARRFSPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPM
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCC
GCDVCYTNHAEADQDDMDTLLTLFGVAGINFIMGVPGADDIMLNYQSTSFHDALYLREVL
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
GLRPAPEFEAWLQRMGIADGSGRLLEPAERQPLLELAHTL
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]