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The map label for this gene is pcaK [H]

Identifier: 73539303

GI number: 73539303

Start: 2277117

End: 2278523

Strand: Reverse

Name: pcaK [H]

Synonym: Reut_B5481

Alternate gene names: 73539303

Gene position: 2278523-2277117 (Counterclockwise)

Preceding gene: 73539304

Following gene: 73539302

Centisome position: 83.58

GC content: 68.3

Gene sequence:

>1407_bases
ATGACCGCCCCATCCGCCCCCAACGGCGCGGCTGCCGCGCCTGCGCGCAACGCCGTCAACGTCCGCGAGTTCATCGACGC
CGCGCGGCTTTCGCCCCTGCAGCTGCGCATCATGCTGCTGTGCTTTGCGATCGTCGCGCTCGATGGCTTCGACATCGCCA
TCATCGGCTTTCTCGCGCCCCATATCCGCAGCGAATGGTCGCTGTCCATGGTCGCATTGGGACCGCTGTTCTCGGCCGGC
CTGCTCGGGCTGATGACCGGGGCCTTCCTCTGCGGCCCGCTGGCCGACCGCATCGGCAGGCGCCGCGTGCTGATGCTGTC
GGTCGCCTGGTTCGGTGCGGCATGCGCTGCTTCCGCTGTGGCACCCGGCATCGGCTGGCTGATCGCGCTGCGCTTCCTGA
CCGGCCTTGGCCTTGGCGGTGCCATGCCCAATGCCATCACGCTGACGGCGGAGTTCAGCCCTTCACGTCGGCGGGGAACC
TTGCTGACCGTGATGTTCTGCGGGTTCTCGCTTGGCTCGGCGATCGGTGGCGTGGTGACGGCCTACCTGATCGACCAGGT
CGGCTGGCGTGGTGTGCTGGCGCTGGGCGGTGCGCTGCCGCTGGCGCTAGTGCCCGTACTCGCGCTGGCATTGCCGGAAT
CGGTGCGCTACCTCGCCACGCGTGAAGGCATGCAGGCGCGGATCGCCGCCATCCTGGCGCGCATCGACGCGCGCCGAATC
ACGGCCGACATGCACTACGTCGCCGACGAACCCGAAGGCCCTGGCTCGCCGGTCAGCCAGCTGTTCGCACCGCGCTACCG
GATGGGGACGCTGTGGCTCTGGCTCGCATTCTTCTGCAGCCTGCTGTTGCTGTACCTGATGATCAACTGGCTGCCGATCC
TGGTTCAGCGCAGCGGCCTTTCGCTGAAAGAGGCATCGATGCTTGCAGGCCTGCTGCAGGGCGGCGGCGTGCTCGGTGCC
ATCGTGCTGGGCATGCTGCTTGACCGGTTCAACCCGTATGCGGTGGTGGCCGGCGCCTACACGCTCGGCGCGATCTCGGT
GGCATGCCTGGCTTCTGTGAGCGCACCGCTGGCGATGGCCGCGGCGATCTTCGTCACGGGCTTCTGCGTCAGCGGATCGC
AAGTCTGCGCCAATGTGCTGGCGTCGGCGTTCTATCCGACGTCGAGCCGGGCAACCGGCGTGGCATGGGCGCTCGGCATC
GGGCGCGTGGGCGCCATCGCAGGCTCCCTCGGCGGAGCCCTGCTGTTGTCGGCGGGATGGAGCAATGCCGCGCTCTATTT
GCTGCTGGCGATCCCGGCCGTCGCCGCGGGGATCAGCATTTTCCGGGTCGGCCGCGTGCAGGTCGCCATAGGGGCCGAGG
AGCGGGACACCACTTCGAACAATCCGCAGGACGTCGTGCAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGGCAACGCCGCGTAGTCCTTCACCGTCGCACAAACCCGTAGCACCCGCACAAGCCGCAGCATAGCGGCTGTGCGCTG
ACAACATCCCAGGAGACAAC

Downstream 100 bases:

>100_bases
ACGCCCGGCAAATCGCTTCTTTTCAGGCGCGCCGGACATCACAGTCAGCGGCCACGCGTTTCCGGAAGTCCCACAGGGGC
GCGATCCAGGCTTCACACCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTAPSAPNGAAAAPARNAVNVREFIDAARLSPLQLRIMLLCFAIVALDGFDIAIIGFLAPHIRSEWSLSMVALGPLFSAG
LLGLMTGAFLCGPLADRIGRRRVLMLSVAWFGAACAASAVAPGIGWLIALRFLTGLGLGGAMPNAITLTAEFSPSRRRGT
LLTVMFCGFSLGSAIGGVVTAYLIDQVGWRGVLALGGALPLALVPVLALALPESVRYLATREGMQARIAAILARIDARRI
TADMHYVADEPEGPGSPVSQLFAPRYRMGTLWLWLAFFCSLLLLYLMINWLPILVQRSGLSLKEASMLAGLLQGGGVLGA
IVLGMLLDRFNPYAVVAGAYTLGAISVACLASVSAPLAMAAAIFVTGFCVSGSQVCANVLASAFYPTSSRATGVAWALGI
GRVGAIAGSLGGALLLSAGWSNAALYLLLAIPAVAAGISIFRVGRVQVAIGAEERDTTSNNPQDVVQA

Sequences:

>Translated_468_residues
MTAPSAPNGAAAAPARNAVNVREFIDAARLSPLQLRIMLLCFAIVALDGFDIAIIGFLAPHIRSEWSLSMVALGPLFSAG
LLGLMTGAFLCGPLADRIGRRRVLMLSVAWFGAACAASAVAPGIGWLIALRFLTGLGLGGAMPNAITLTAEFSPSRRRGT
LLTVMFCGFSLGSAIGGVVTAYLIDQVGWRGVLALGGALPLALVPVLALALPESVRYLATREGMQARIAAILARIDARRI
TADMHYVADEPEGPGSPVSQLFAPRYRMGTLWLWLAFFCSLLLLYLMINWLPILVQRSGLSLKEASMLAGLLQGGGVLGA
IVLGMLLDRFNPYAVVAGAYTLGAISVACLASVSAPLAMAAAIFVTGFCVSGSQVCANVLASAFYPTSSRATGVAWALGI
GRVGAIAGSLGGALLLSAGWSNAALYLLLAIPAVAAGISIFRVGRVQVAIGAEERDTTSNNPQDVVQA
>Mature_467_residues
TAPSAPNGAAAAPARNAVNVREFIDAARLSPLQLRIMLLCFAIVALDGFDIAIIGFLAPHIRSEWSLSMVALGPLFSAGL
LGLMTGAFLCGPLADRIGRRRVLMLSVAWFGAACAASAVAPGIGWLIALRFLTGLGLGGAMPNAITLTAEFSPSRRRGTL
LTVMFCGFSLGSAIGGVVTAYLIDQVGWRGVLALGGALPLALVPVLALALPESVRYLATREGMQARIAAILARIDARRIT
ADMHYVADEPEGPGSPVSQLFAPRYRMGTLWLWLAFFCSLLLLYLMINWLPILVQRSGLSLKEASMLAGLLQGGGVLGAI
VLGMLLDRFNPYAVVAGAYTLGAISVACLASVSAPLAMAAAIFVTGFCVSGSQVCANVLASAFYPTSSRATGVAWALGIG
RVGAIAGSLGGALLLSAGWSNAALYLLLAIPAVAAGISIFRVGRVQVAIGAEERDTTSNNPQDVVQA

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=459, Percent_Identity=27.0152505446623, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI8923870, Length=367, Percent_Identity=26.158038147139, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI166064021, Length=367, Percent_Identity=26.158038147139, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI213021148, Length=231, Percent_Identity=25.5411255411255, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI7662270, Length=199, Percent_Identity=28.643216080402, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI20070188, Length=399, Percent_Identity=25.5639097744361, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI24497476, Length=399, Percent_Identity=25.5639097744361, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI262399383, Length=174, Percent_Identity=28.735632183908, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI11415038, Length=339, Percent_Identity=28.6135693215339, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI216548223, Length=135, Percent_Identity=34.8148148148148, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI296080719, Length=342, Percent_Identity=25.1461988304094, Blast_Score=65, Evalue=1e-10,
Organism=Homo sapiens, GI24497499, Length=342, Percent_Identity=25.1461988304094, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI87081723, Length=412, Percent_Identity=34.2233009708738, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082159, Length=397, Percent_Identity=28.463476070529, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786229, Length=449, Percent_Identity=23.3853006681514, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788074, Length=356, Percent_Identity=25.8426966292135, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082231, Length=177, Percent_Identity=27.683615819209, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789152, Length=409, Percent_Identity=23.7163814180929, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788068, Length=424, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082404, Length=206, Percent_Identity=21.3592233009709, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI32564663, Length=434, Percent_Identity=23.963133640553, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320595, Length=231, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922874, Length=414, Percent_Identity=24.3961352657005, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24640198, Length=469, Percent_Identity=23.6673773987207, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640196, Length=469, Percent_Identity=23.6673773987207, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640200, Length=469, Percent_Identity=23.6673773987207, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48455; Mature: 48324

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSAPNGAAAAPARNAVNVREFIDAARLSPLQLRIMLLCFAIVALDGFDIAIIGFLAP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HIRSEWSLSMVALGPLFSAGLLGLMTGAFLCGPLADRIGRRRVLMLSVAWFGAACAASAV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
APGIGWLIALRFLTGLGLGGAMPNAITLTAEFSPSRRRGTLLTVMFCGFSLGSAIGGVVT
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AYLIDQVGWRGVLALGGALPLALVPVLALALPESVRYLATREGMQARIAAILARIDARRI
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TADMHYVADEPEGPGSPVSQLFAPRYRMGTLWLWLAFFCSLLLLYLMINWLPILVQRSGL
HHHHHEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLKEASMLAGLLQGGGVLGAIVLGMLLDRFNPYAVVAGAYTLGAISVACLASVSAPLAMA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AAIFVTGFCVSGSQVCANVLASAFYPTSSRATGVAWALGIGRVGAIAGSLGGALLLSAGW
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEEECCC
SNAALYLLLAIPAVAAGISIFRVGRVQVAIGAEERDTTSNNPQDVVQA
CHHHHHHHHHHHHHHHHHHHEEECEEEEEECCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
TAPSAPNGAAAAPARNAVNVREFIDAARLSPLQLRIMLLCFAIVALDGFDIAIIGFLAP
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HIRSEWSLSMVALGPLFSAGLLGLMTGAFLCGPLADRIGRRRVLMLSVAWFGAACAASAV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
APGIGWLIALRFLTGLGLGGAMPNAITLTAEFSPSRRRGTLLTVMFCGFSLGSAIGGVVT
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AYLIDQVGWRGVLALGGALPLALVPVLALALPESVRYLATREGMQARIAAILARIDARRI
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TADMHYVADEPEGPGSPVSQLFAPRYRMGTLWLWLAFFCSLLLLYLMINWLPILVQRSGL
HHHHHEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLKEASMLAGLLQGGGVLGAIVLGMLLDRFNPYAVVAGAYTLGAISVACLASVSAPLAMA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AAIFVTGFCVSGSQVCANVLASAFYPTSSRATGVAWALGIGRVGAIAGSLGGALLLSAGW
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEEECCC
SNAALYLLLAIPAVAAGISIFRVGRVQVAIGAEERDTTSNNPQDVVQA
CHHHHHHHHHHHHHHHHHHHEEECEEEEEECCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]