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The map label for this gene is yhcK [H]

Identifier: 73539291

GI number: 73539291

Start: 2259711

End: 2260970

Strand: Direct

Name: yhcK [H]

Synonym: Reut_B5469

Alternate gene names: 73539291

Gene position: 2259711-2260970 (Clockwise)

Preceding gene: 73539288

Following gene: 73539298

Centisome position: 82.89

GC content: 64.6

Gene sequence:

>1260_bases
ATGCCCCGCCCGCGAGGCCTGCCATATTTCCCACGTAGAATCTCCCTGCCGCCGCCCATGTACCGGCGGCGCAATGTCTT
TACATTTGGGATGCACGTCGATCTTCTCACCCTTTACTTTCTGGCAATCGGAACACTCCTCGCTAGCGCCGGAATGATGT
TCTGGGAGCGCAGGGCCAATCCCGGGCGCAGCAGGTCATTGCGGATCCTTGCAACGGGGTTCACGACGCTCGCCGCAGGG
TGCGCGGTGGTGCTGGTTCGCCGCAGTTTGCCCGAGGTGCTCGGTTCAGCCGTCGCCAATCTCGTCATGCTCAGCGGCTA
CCTGCTTGTCCTCGGTGGCGTCGCCTCGTTGCGCGGGCGGCGCTACCGTGCCGCCTCATCCGGCTTGCTGGTGGCCATGG
CACTCGTGTGGATCGTTGCGGGTGCGCGTTGGAAGGACATCATCTGGAGCTACATCAGCGCATTCCCGCTGGCGGTCGTG
AGCGCCGCGACGGCGTGGGAAATGCTGCGCTGTGAGGCGATGAAGTCGCTGCATGCGCGGTATGTTGTCGTGGCGGTGAC
CGGCGTGCACGCCGTGCTTTACACGGGCCGGTCGTTCGTGTTGCCCTGGCTTGTGGGGGCGTACGGGCCTTCCGTCAGCG
CGATCGCGAGCAATGTCACGCTCTACGAGGGCGTGCTGTACTCGGTGCTGCTGCCGATGGCCCTGATCAGGCTGATTCGC
GAGGAAACCCACAGCCAGCTCCTGCTCGAATCGCAGACCGACTACCTGACCCGTCTCGGCAATCGGCGGTGGTTCTTCGA
ACAGGGCAGCCGCATCCTCGACGGTGGCGCAAGCGGCCCGGTTTCCGTGCTCGCGTTCGATCTCGACCATTTCAAGGCCA
TCAACGACCTGTACGGCCACCAGACCGGCGACAAGGTGCTGAAGTCATTCGCCGAGACTGCCCGCAGCGTAGTCGGCGCC
GACGCGATTCTCGCCCGTATCGGCGGCGAAGAGTTTGCCGCGGTGCTGTCTGGCCATGACGCAGCGCGCGCCCAGTCACT
GGGCGAGAGCGTCGCCAGGCTTTTTGCCGAGACGACTTCGAGCCGTACCGACAGTCTTGGCGTGCCGGCGACGGTCAGCA
TTGGGCTGGCGCATTTCGAGGACGAGGTGCCCGCGCTGGTGGAAGGATTGGCTGCGGCGGATCAGGCGCTGTATCGCGCG
AAGTCGCTGGGCGGCAATCGACTTGAGCTGGCGCAGCCGAAGCTCCATCCCTTTTCCTGA

Upstream 100 bases:

>100_bases
TACGACGACATGACGAGTCAGGGCCCCCTGGGTGTGACATCGGCGGGAGATTTTTTTGGAGATTCCGCAGAACCCGCTCC
GACGGGGGCTGTCGCCCAAG

Downstream 100 bases:

>100_bases
AGGACGCAGCGCGATGATGACTGCCGACAGCGGCGCGCCAACTATCTGCCGGCGGCCGCTGCGCTGGCAACGCTAGTCCA
ATTCCTTGCCCTCAGTTTCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPRPRGLPYFPRRISLPPPMYRRRNVFTFGMHVDLLTLYFLAIGTLLASAGMMFWERRANPGRSRSLRILATGFTTLAAG
CAVVLVRRSLPEVLGSAVANLVMLSGYLLVLGGVASLRGRRYRAASSGLLVAMALVWIVAGARWKDIIWSYISAFPLAVV
SAATAWEMLRCEAMKSLHARYVVVAVTGVHAVLYTGRSFVLPWLVGAYGPSVSAIASNVTLYEGVLYSVLLPMALIRLIR
EETHSQLLLESQTDYLTRLGNRRWFFEQGSRILDGGASGPVSVLAFDLDHFKAINDLYGHQTGDKVLKSFAETARSVVGA
DAILARIGGEEFAAVLSGHDAARAQSLGESVARLFAETTSSRTDSLGVPATVSIGLAHFEDEVPALVEGLAAADQALYRA
KSLGGNRLELAQPKLHPFS

Sequences:

>Translated_419_residues
MPRPRGLPYFPRRISLPPPMYRRRNVFTFGMHVDLLTLYFLAIGTLLASAGMMFWERRANPGRSRSLRILATGFTTLAAG
CAVVLVRRSLPEVLGSAVANLVMLSGYLLVLGGVASLRGRRYRAASSGLLVAMALVWIVAGARWKDIIWSYISAFPLAVV
SAATAWEMLRCEAMKSLHARYVVVAVTGVHAVLYTGRSFVLPWLVGAYGPSVSAIASNVTLYEGVLYSVLLPMALIRLIR
EETHSQLLLESQTDYLTRLGNRRWFFEQGSRILDGGASGPVSVLAFDLDHFKAINDLYGHQTGDKVLKSFAETARSVVGA
DAILARIGGEEFAAVLSGHDAARAQSLGESVARLFAETTSSRTDSLGVPATVSIGLAHFEDEVPALVEGLAAADQALYRA
KSLGGNRLELAQPKLHPFS
>Mature_418_residues
PRPRGLPYFPRRISLPPPMYRRRNVFTFGMHVDLLTLYFLAIGTLLASAGMMFWERRANPGRSRSLRILATGFTTLAAGC
AVVLVRRSLPEVLGSAVANLVMLSGYLLVLGGVASLRGRRYRAASSGLLVAMALVWIVAGARWKDIIWSYISAFPLAVVS
AATAWEMLRCEAMKSLHARYVVVAVTGVHAVLYTGRSFVLPWLVGAYGPSVSAIASNVTLYEGVLYSVLLPMALIRLIRE
ETHSQLLLESQTDYLTRLGNRRWFFEQGSRILDGGASGPVSVLAFDLDHFKAINDLYGHQTGDKVLKSFAETARSVVGAD
AILARIGGEEFAAVLSGHDAARAQSLGESVARLFAETTSSRTDSLGVPATVSIGLAHFEDEVPALVEGLAAADQALYRAK
SLGGNRLELAQPKLHPFS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=157, Percent_Identity=34.3949044585987, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=37.7142857142857, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=188, Percent_Identity=35.6382978723404, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=157, Percent_Identity=33.1210191082803, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=166, Percent_Identity=31.9277108433735, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=30.0578034682081, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081977, Length=200, Percent_Identity=29.5, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=30.8571428571429, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787541, Length=181, Percent_Identity=28.1767955801105, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=29.8136645962733, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 45377; Mature: 45246

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPRGLPYFPRRISLPPPMYRRRNVFTFGMHVDLLTLYFLAIGTLLASAGMMFWERRAN
CCCCCCCCCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGRSRSLRILATGFTTLAAGCAVVLVRRSLPEVLGSAVANLVMLSGYLLVLGGVASLRGR
CCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYRAASSGLLVAMALVWIVAGARWKDIIWSYISAFPLAVVSAATAWEMLRCEAMKSLHAR
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YVVVAVTGVHAVLYTGRSFVLPWLVGAYGPSVSAIASNVTLYEGVLYSVLLPMALIRLIR
EEEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EETHSQLLLESQTDYLTRLGNRRWFFEQGSRILDGGASGPVSVLAFDLDHFKAINDLYGH
HHHHHHHHHHHHHHHHHHHCCCCEEHHCCCEEECCCCCCCCEEEEECHHHHHHHHHHHCC
QTGDKVLKSFAETARSVVGADAILARIGGEEFAAVLSGHDAARAQSLGESVARLFAETTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
SRTDSLGVPATVSIGLAHFEDEVPALVEGLAAADQALYRAKSLGGNRLELAQPKLHPFS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
PRPRGLPYFPRRISLPPPMYRRRNVFTFGMHVDLLTLYFLAIGTLLASAGMMFWERRAN
CCCCCCCCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGRSRSLRILATGFTTLAAGCAVVLVRRSLPEVLGSAVANLVMLSGYLLVLGGVASLRGR
CCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYRAASSGLLVAMALVWIVAGARWKDIIWSYISAFPLAVVSAATAWEMLRCEAMKSLHAR
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YVVVAVTGVHAVLYTGRSFVLPWLVGAYGPSVSAIASNVTLYEGVLYSVLLPMALIRLIR
EEEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EETHSQLLLESQTDYLTRLGNRRWFFEQGSRILDGGASGPVSVLAFDLDHFKAINDLYGH
HHHHHHHHHHHHHHHHHHHCCCCEEHHCCCEEECCCCCCCCEEEEECHHHHHHHHHHHCC
QTGDKVLKSFAETARSVVGADAILARIGGEEFAAVLSGHDAARAQSLGESVARLFAETTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
SRTDSLGVPATVSIGLAHFEDEVPALVEGLAAADQALYRAKSLGGNRLELAQPKLHPFS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]