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The map label for this gene is pilR [H]

Identifier: 73539239

GI number: 73539239

Start: 2204476

End: 2205846

Strand: Direct

Name: pilR [H]

Synonym: Reut_B5417

Alternate gene names: 73539239

Gene position: 2204476-2205846 (Clockwise)

Preceding gene: 73539238

Following gene: 73539242

Centisome position: 80.86

GC content: 65.57

Gene sequence:

>1371_bases
ATGAAAACCGACCGCTGGGCCTACGAAAACCTCGAAGTCTACGTCTGGGAAGGCAAGTACGAGATTGCCGACCGCGTGAC
GCGTTTCCTGACGCCGCTCGGTGTGGAAGTGATCCGCGCGGGGGCGCTGGAAGCCCTGCCCGAAGGAGCCCGCACCAGAC
CGTGCCTGGCGGTGATCAGCGTCTCGGTGATCGGCGCGGCACGCTTCACCGCGCTGGACTGGGAAGCCGCGCATGGCATG
CCGGTGATCTGGGTCGCCGCACCGGGTCGCGAGTCGGATCCCGGACGCTTCCCGCCCGAATACGCCCACATCCTGCCCGA
TGACTTTACCGGCGCCGACCTGCGCAACCAGATCGGCAAGCTGTTGCCGCAGATGATGGCGACAGACGACCACGGCGACC
GGCACGCCGAACTGGTAGCAGGTTCGCCCGCCATGCAGCGACTGCTGTCGCAGGTGGATACGTTCGCGGACTTCGGCAGC
AATGTGCTGCTGTACGGCGAGACCGGGGCCGGCAAAGAGCGCATTGCGCAACTGTTCCACGAACGCAACAAGACATATGG
CAAGGGCCCGTTCGTGCCCGTGAACTGCGGCGCGATACCGGACGGGTTGTTCGAGTCACAATTCTTCGGCCACGCCAAGG
GCGCGTTCACCGGCGCATCGTTCGCGCACCGCGGCTTCTTCGAACAGGCCAACGGCGGCACGCTGTTCCTGGACGAGATC
GGCGACCTGCCCTTGTTCCAGCAGGTCAAGCTGCTGCGCGTGCTCGAAGAGAACGTGGTGACACGGCTGGGCTCGACCAT
GAACGTAAAGCTCGATTTCCGCCTGGTTGCCGCGACCAACAAGGATCTGCGCGATGCGGTACGGCAGGGGCGTTTTCGCG
CTGACCTGTTCTTCCGCCTGGCGGTGATCGAGATGCGTATTCCGAGCCTGGAAGAGCGCGGCATGGCCGACAAGGTGGCG
CTGCTGACCGCCTTCCTGCGGCACATGATGGGGGCGGCGGCGCTGGACGCCCTGCCGCCGATGCCGGACTGGATCAAGGG
CGCCGTCGGCACGGCTTACTTTGCCGGTAATGTGCGCGAGCTTCGGAACCTGGCCGAGCGCATGGGCATTGCCGTCCGGC
AAGCCGGAGACTGGGACCCTGAGCGAATCCGCCCGCTTTTCAGCATGCTGCACCCCGGCGCGTTCGATGGCAGCGACCGC
CATGCCGACTGGCGCAACGACGCGGATGAGCGCCGCCGTATCCTGGCCGCGCTCGACGCCAACGGCTGGCGCCGTCAGGA
TACGGCTGCCAGCCTGGGCATTAGCCGCAAGGTGCTGTGGGAGAAGATGCGCAAGTATCAGATTGCCGACAGCGAGGCTG
AACCGGCGTAA

Upstream 100 bases:

>100_bases
TGGGCACCGCCGACCTCTGGCTGCACAACATCGACTGCAACAACGCCGAACTGGTGTACTGAGCCATGATCGCGACCTCT
CGCCTGATGGCAGGAATCGA

Downstream 100 bases:

>100_bases
AGACCAGGACGCTAGTTAAGGTTCGTGATGTAGCTCGCCAGGTTGCGGATATCCTCGTCGCTCAGGTTGCGCGAGACGCT
GGTCATGTTGCCCGCATCGT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKTDRWAYENLEVYVWEGKYEIADRVTRFLTPLGVEVIRAGALEALPEGARTRPCLAVISVSVIGAARFTALDWEAAHGM
PVIWVAAPGRESDPGRFPPEYAHILPDDFTGADLRNQIGKLLPQMMATDDHGDRHAELVAGSPAMQRLLSQVDTFADFGS
NVLLYGETGAGKERIAQLFHERNKTYGKGPFVPVNCGAIPDGLFESQFFGHAKGAFTGASFAHRGFFEQANGGTLFLDEI
GDLPLFQQVKLLRVLEENVVTRLGSTMNVKLDFRLVAATNKDLRDAVRQGRFRADLFFRLAVIEMRIPSLEERGMADKVA
LLTAFLRHMMGAAALDALPPMPDWIKGAVGTAYFAGNVRELRNLAERMGIAVRQAGDWDPERIRPLFSMLHPGAFDGSDR
HADWRNDADERRRILAALDANGWRRQDTAASLGISRKVLWEKMRKYQIADSEAEPA

Sequences:

>Translated_456_residues
MKTDRWAYENLEVYVWEGKYEIADRVTRFLTPLGVEVIRAGALEALPEGARTRPCLAVISVSVIGAARFTALDWEAAHGM
PVIWVAAPGRESDPGRFPPEYAHILPDDFTGADLRNQIGKLLPQMMATDDHGDRHAELVAGSPAMQRLLSQVDTFADFGS
NVLLYGETGAGKERIAQLFHERNKTYGKGPFVPVNCGAIPDGLFESQFFGHAKGAFTGASFAHRGFFEQANGGTLFLDEI
GDLPLFQQVKLLRVLEENVVTRLGSTMNVKLDFRLVAATNKDLRDAVRQGRFRADLFFRLAVIEMRIPSLEERGMADKVA
LLTAFLRHMMGAAALDALPPMPDWIKGAVGTAYFAGNVRELRNLAERMGIAVRQAGDWDPERIRPLFSMLHPGAFDGSDR
HADWRNDADERRRILAALDANGWRRQDTAASLGISRKVLWEKMRKYQIADSEAEPA
>Mature_456_residues
MKTDRWAYENLEVYVWEGKYEIADRVTRFLTPLGVEVIRAGALEALPEGARTRPCLAVISVSVIGAARFTALDWEAAHGM
PVIWVAAPGRESDPGRFPPEYAHILPDDFTGADLRNQIGKLLPQMMATDDHGDRHAELVAGSPAMQRLLSQVDTFADFGS
NVLLYGETGAGKERIAQLFHERNKTYGKGPFVPVNCGAIPDGLFESQFFGHAKGAFTGASFAHRGFFEQANGGTLFLDEI
GDLPLFQQVKLLRVLEENVVTRLGSTMNVKLDFRLVAATNKDLRDAVRQGRFRADLFFRLAVIEMRIPSLEERGMADKVA
LLTAFLRHMMGAAALDALPPMPDWIKGAVGTAYFAGNVRELRNLAERMGIAVRQAGDWDPERIRPLFSMLHPGAFDGSDR
HADWRNDADERRRILAALDANGWRRQDTAASLGISRKVLWEKMRKYQIADSEAEPA

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=316, Percent_Identity=38.6075949367089, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87082117, Length=359, Percent_Identity=37.883008356546, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1790437, Length=309, Percent_Identity=38.5113268608414, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788905, Length=293, Percent_Identity=40.6143344709898, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1786524, Length=403, Percent_Identity=34.2431761786601, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=33.8414634146341, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=41.2698412698413, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790299, Length=228, Percent_Identity=42.5438596491228, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789087, Length=237, Percent_Identity=41.7721518987342, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=39.5652173913043, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1787583, Length=346, Percent_Identity=30.3468208092486, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=36.4406779661017, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081858, Length=293, Percent_Identity=30.3754266211604, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50665; Mature: 50665

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDRWAYENLEVYVWEGKYEIADRVTRFLTPLGVEVIRAGALEALPEGARTRPCLAVIS
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VSVIGAARFTALDWEAAHGMPVIWVAAPGRESDPGRFPPEYAHILPDDFTGADLRNQIGK
HHHHHHHHHEEECCHHCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHH
LLPQMMATDDHGDRHAELVAGSPAMQRLLSQVDTFADFGSNVLLYGETGAGKERIAQLFH
HHHHHHCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
ERNKTYGKGPFVPVNCGAIPDGLFESQFFGHAKGAFTGASFAHRGFFEQANGGTLFLDEI
HHCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEECCCC
GDLPLFQQVKLLRVLEENVVTRLGSTMNVKLDFRLVAATNKDLRDAVRQGRFRADLFFRL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHH
AVIEMRIPSLEERGMADKVALLTAFLRHMMGAAALDALPPMPDWIKGAVGTAYFAGNVRE
HHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
LRNLAERMGIAVRQAGDWDPERIRPLFSMLHPGAFDGSDRHADWRNDADERRRILAALDA
HHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NGWRRQDTAASLGISRKVLWEKMRKYQIADSEAEPA
CCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTDRWAYENLEVYVWEGKYEIADRVTRFLTPLGVEVIRAGALEALPEGARTRPCLAVIS
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VSVIGAARFTALDWEAAHGMPVIWVAAPGRESDPGRFPPEYAHILPDDFTGADLRNQIGK
HHHHHHHHHEEECCHHCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHH
LLPQMMATDDHGDRHAELVAGSPAMQRLLSQVDTFADFGSNVLLYGETGAGKERIAQLFH
HHHHHHCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
ERNKTYGKGPFVPVNCGAIPDGLFESQFFGHAKGAFTGASFAHRGFFEQANGGTLFLDEI
HHCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEECCCC
GDLPLFQQVKLLRVLEENVVTRLGSTMNVKLDFRLVAATNKDLRDAVRQGRFRADLFFRL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHH
AVIEMRIPSLEERGMADKVALLTAFLRHMMGAAALDALPPMPDWIKGAVGTAYFAGNVRE
HHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
LRNLAERMGIAVRQAGDWDPERIRPLFSMLHPGAFDGSDRHADWRNDADERRRILAALDA
HHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NGWRRQDTAASLGISRKVLWEKMRKYQIADSEAEPA
CCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]