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The map label for this gene is leuC1 [H]

Identifier: 73539209

GI number: 73539209

Start: 2171462

End: 2172766

Strand: Reverse

Name: leuC1 [H]

Synonym: Reut_B5387

Alternate gene names: 73539209

Gene position: 2172766-2171462 (Counterclockwise)

Preceding gene: 73539210

Following gene: 73539208

Centisome position: 79.7

GC content: 71.42

Gene sequence:

>1305_bases
ATGAACGTCGCCGCAGCATCCGGGCACATCCCTCCCGCCACGCTGGCCCAGAAGCTGGTGGCGCGAGCCGCCGGGCTCGC
GCACGCCGAGCCCGGTTCACTCGCGATGTGCCGCGTCGACCTCGCGATGTCGCACGACTCCAGCGGACCGCGCCGCGTCG
CGCCGTTGCTGGAGGCGCTCGGCACGACCGTGTGGGATCCGTCGCGCTACGTGGTCGTGACCGACCATTTCGTGCCGGCC
TCCGATCCCGCTGCTGAAGCCATCCTGCACTTCACGCGCGACTGGACGCGCAGCAGCGGCGCGCATCTGATCGACGGAGA
AGGCATCTGCCACGTGGTACTACCCGAGCACGGCCACGTGCTGCCCGGCCGCCTGATCGTGGGCGGCGACAGCCACAGCC
CGACCGGTGGCGCGTTCGGCGCGTATATGTTCGGCGTGGGCGCCACGGAGATGGCCGGCGTGCTCGCCACGGGCGAAATC
TGGCTGCGCGTGCCGCAGACCATCCGGCTGCAGTGGGACGGCGCGCTGGCGCCCGGTGTCTGCGCCAAGGACATGATGCT
GTTCCTGTGCGCGAAGCTGGGACTGGGCGGCGGCCGCTACGAGGCGATCGAGTATGCCGGCAGCGCCGTGGCCGCGCTGC
CCATGCAGGAACGCATGACGCTGGCCAATATGAGCGCGGAGCTCGGCGCGCAGACCGGGCTGGTCGCGCCCGATGTCGTC
ACCCTGGACTGGCTTGCCGCTGCTGGCGTACCGGCGCAAACGCTGGCGGAAATTGACCTCGCACACTGGCGCAGCGATAC
CGATGCGCCACTGCTGGCGAATCATGTCTTCGATGCCGCCGCACTGACACCGCAGGTGGCCGCTCCCCATTCGCCCGCCA
ACAGCGCGCCGGTGGATCAGGCCGCCGGCGAATCCGTGCAGATCGCCTACCTTGGCGCCTGCACCGGCGCCAAGCTCGAG
GACCTGCGCATGGCCGCCAGCGTGCTGCGCGGACGCCGCGTGGCGCCAGGCGTCAGCCTGCAGGTAGCACCCGCTTCCGC
GCGCGACCAGCAGCAGGCGCAGCGCGAAGGCACGCTGGCCGTACTGGCCGACGCGGGCGCCGACCTGCTGCCCAATGCCT
GCGGAGCCTGCGCCGGCTATGGCGCGGCACGCTTTCCCGCCGGCAGCCGGGCGATTGCCTCGACCGCGCGCAACTTCTCC
GGGCGCATGGGCGATGCCGGCAGCGCGGTGTGGCTCGCGTCCCCGCTGACTGTAGCGGCCAGCGCCGTGACCGGACGCAT
CACCGACCCACGTACCCTGCTCTGA

Upstream 100 bases:

>100_bases
CACAGCCGCTCGGCACCTCGCCGGAATCGTTCGCCGCGTTCGTGCGGCAGGACGTCAAGCGCTGGGCCGAGGTCGTGAAA
TATTCAGGCGCCACGCCAGA

Downstream 100 bases:

>100_bases
AGCCGTATTTCCATTTCCGCATGACCGAGCCTTCCGTTCCCGCGTCTGCCGGCCGCATCTGGCGCTTTGGCGACAACATC
GACACCGACGCCATGGCACC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNVAAASGHIPPATLAQKLVARAAGLAHAEPGSLAMCRVDLAMSHDSSGPRRVAPLLEALGTTVWDPSRYVVVTDHFVPA
SDPAAEAILHFTRDWTRSSGAHLIDGEGICHVVLPEHGHVLPGRLIVGGDSHSPTGGAFGAYMFGVGATEMAGVLATGEI
WLRVPQTIRLQWDGALAPGVCAKDMMLFLCAKLGLGGGRYEAIEYAGSAVAALPMQERMTLANMSAELGAQTGLVAPDVV
TLDWLAAAGVPAQTLAEIDLAHWRSDTDAPLLANHVFDAAALTPQVAAPHSPANSAPVDQAAGESVQIAYLGACTGAKLE
DLRMAASVLRGRRVAPGVSLQVAPASARDQQQAQREGTLAVLADAGADLLPNACGACAGYGAARFPAGSRAIASTARNFS
GRMGDAGSAVWLASPLTVAASAVTGRITDPRTLL

Sequences:

>Translated_434_residues
MNVAAASGHIPPATLAQKLVARAAGLAHAEPGSLAMCRVDLAMSHDSSGPRRVAPLLEALGTTVWDPSRYVVVTDHFVPA
SDPAAEAILHFTRDWTRSSGAHLIDGEGICHVVLPEHGHVLPGRLIVGGDSHSPTGGAFGAYMFGVGATEMAGVLATGEI
WLRVPQTIRLQWDGALAPGVCAKDMMLFLCAKLGLGGGRYEAIEYAGSAVAALPMQERMTLANMSAELGAQTGLVAPDVV
TLDWLAAAGVPAQTLAEIDLAHWRSDTDAPLLANHVFDAAALTPQVAAPHSPANSAPVDQAAGESVQIAYLGACTGAKLE
DLRMAASVLRGRRVAPGVSLQVAPASARDQQQAQREGTLAVLADAGADLLPNACGACAGYGAARFPAGSRAIASTARNFS
GRMGDAGSAVWLASPLTVAASAVTGRITDPRTLL
>Mature_434_residues
MNVAAASGHIPPATLAQKLVARAAGLAHAEPGSLAMCRVDLAMSHDSSGPRRVAPLLEALGTTVWDPSRYVVVTDHFVPA
SDPAAEAILHFTRDWTRSSGAHLIDGEGICHVVLPEHGHVLPGRLIVGGDSHSPTGGAFGAYMFGVGATEMAGVLATGEI
WLRVPQTIRLQWDGALAPGVCAKDMMLFLCAKLGLGGGRYEAIEYAGSAVAALPMQERMTLANMSAELGAQTGLVAPDVV
TLDWLAAAGVPAQTLAEIDLAHWRSDTDAPLLANHVFDAAALTPQVAAPHSPANSAPVDQAAGESVQIAYLGACTGAKLE
DLRMAASVLRGRRVAPGVSLQVAPASARDQQQAQREGTLAVLADAGADLLPNACGACAGYGAARFPAGSRAIASTARNFS
GRMGDAGSAVWLASPLTVAASAVTGRITDPRTLL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=350, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8659555, Length=390, Percent_Identity=25.3846153846154, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=31.5450643776824, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI2367097, Length=457, Percent_Identity=25.164113785558, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787531, Length=390, Percent_Identity=24.8717948717949, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081781, Length=346, Percent_Identity=25.7225433526012, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32564738, Length=343, Percent_Identity=29.4460641399417, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25149337, Length=343, Percent_Identity=29.4460641399417, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=287, Percent_Identity=27.8745644599303, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17568399, Length=442, Percent_Identity=22.8506787330317, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=418, Percent_Identity=34.9282296650718, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321429, Length=478, Percent_Identity=29.2887029288703, Blast_Score=186, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323335, Length=342, Percent_Identity=28.3625730994152, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=371, Percent_Identity=26.9541778975741, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=344, Percent_Identity=28.7790697674419, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281365315, Length=344, Percent_Identity=28.7790697674419, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864292, Length=344, Percent_Identity=28.7790697674419, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571643, Length=345, Percent_Identity=27.8260869565217, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44479; Mature: 44479

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAAASGHIPPATLAQKLVARAAGLAHAEPGSLAMCRVDLAMSHDSSGPRRVAPLLEAL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHH
GTTVWDPSRYVVVTDHFVPASDPAAEAILHFTRDWTRSSGAHLIDGEGICHVVLPEHGHV
CCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCC
LPGRLIVGGDSHSPTGGAFGAYMFGVGATEMAGVLATGEIWLRVPQTIRLQWDGALAPGV
CCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHEEEECEEEEECCCEEEEEECCCCCCCH
CAKDMMLFLCAKLGLGGGRYEAIEYAGSAVAALPMQERMTLANMSAELGAQTGLVAPDVV
HHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHH
TLDWLAAAGVPAQTLAEIDLAHWRSDTDAPLLANHVFDAAALTPQVAAPHSPANSAPVDQ
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
AAGESVQIAYLGACTGAKLEDLRMAASVLRGRRVAPGVSLQVAPASARDQQQAQREGTLA
HCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHCCCEE
VLADAGADLLPNACGACAGYGAARFPAGSRAIASTARNFSGRMGDAGSAVWLASPLTVAA
EEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
SAVTGRITDPRTLL
HHHCCCCCCCCCCC
>Mature Secondary Structure
MNVAAASGHIPPATLAQKLVARAAGLAHAEPGSLAMCRVDLAMSHDSSGPRRVAPLLEAL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHH
GTTVWDPSRYVVVTDHFVPASDPAAEAILHFTRDWTRSSGAHLIDGEGICHVVLPEHGHV
CCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCC
LPGRLIVGGDSHSPTGGAFGAYMFGVGATEMAGVLATGEIWLRVPQTIRLQWDGALAPGV
CCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHEEEECEEEEECCCEEEEEECCCCCCCH
CAKDMMLFLCAKLGLGGGRYEAIEYAGSAVAALPMQERMTLANMSAELGAQTGLVAPDVV
HHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHH
TLDWLAAAGVPAQTLAEIDLAHWRSDTDAPLLANHVFDAAALTPQVAAPHSPANSAPVDQ
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
AAGESVQIAYLGACTGAKLEDLRMAASVLRGRRVAPGVSLQVAPASARDQQQAQREGTLA
HCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHCCCEE
VLADAGADLLPNACGACAGYGAARFPAGSRAIASTARNFSGRMGDAGSAVWLASPLTVAA
EEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
SAVTGRITDPRTLL
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA