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The map label for this gene is leuD [H]

Identifier: 73539208

GI number: 73539208

Start: 2170935

End: 2171441

Strand: Reverse

Name: leuD [H]

Synonym: Reut_B5386

Alternate gene names: 73539208

Gene position: 2171441-2170935 (Counterclockwise)

Preceding gene: 73539209

Following gene: 73539205

Centisome position: 79.65

GC content: 67.46

Gene sequence:

>507_bases
ATGACCGAGCCTTCCGTTCCCGCGTCTGCCGGCCGCATCTGGCGCTTTGGCGACAACATCGACACCGACGCCATGGCACC
CGCGGTATGGATGAAGGCCGCCCCAGAAGCCCTTGCCGCGCATTGCCTCAAAAGCCTTCGCCCCGAGTTCCCGGACCAGA
TGCAGCCAGGTGACGTGGTGGTAGCAGGCGCCAACTTCGGCATCGGCTCTTCGCGCGAGCAGGCGCCGCAGGCGCTGCGC
CAGCTGAGGGTGCGCGCCGTGGTGGCGCCCTCGTTCGGCGGGCTGTTCTACCGCAACGCCATCAACCTCGGCCTGCCGGT
GCTGGTATGCCCCGACACCAGCCGCCTGGCGGATGGCATGCTTGCGACGCTGGATCTGGACCGTGCCGTGCTGCAGCTCA
GCGACGGAACAACCGTGGCGTGCGAACCCGTACCGGCATTCCTGCGCGAGATCCTGCAGGCAGGCGGCTTGATTCCCCAC
CTGAAGGCGAAGCTGGCAAGCCGTTGA

Upstream 100 bases:

>100_bases
CGGTGTGGCTCGCGTCCCCGCTGACTGTAGCGGCCAGCGCCGTGACCGGACGCATCACCGACCCACGTACCCTGCTCTGA
AGCCGTATTTCCATTTCCGC

Downstream 100 bases:

>100_bases
CGGGTTAGCCGGCGCTTACACCGCCCACATTGCCGCCTCGCGCAGCAATGCCAGCGCCTCGTCGTCGCTGGTCTGGGTGA
AATCCAGATAGAAGGCACCC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTEPSVPASAGRIWRFGDNIDTDAMAPAVWMKAAPEALAAHCLKSLRPEFPDQMQPGDVVVAGANFGIGSSREQAPQALR
QLRVRAVVAPSFGGLFYRNAINLGLPVLVCPDTSRLADGMLATLDLDRAVLQLSDGTTVACEPVPAFLREILQAGGLIPH
LKAKLASR

Sequences:

>Translated_168_residues
MTEPSVPASAGRIWRFGDNIDTDAMAPAVWMKAAPEALAAHCLKSLRPEFPDQMQPGDVVVAGANFGIGSSREQAPQALR
QLRVRAVVAPSFGGLFYRNAINLGLPVLVCPDTSRLADGMLATLDLDRAVLQLSDGTTVACEPVPAFLREILQAGGLIPH
LKAKLASR
>Mature_167_residues
TEPSVPASAGRIWRFGDNIDTDAMAPAVWMKAAPEALAAHCLKSLRPEFPDQMQPGDVVVAGANFGIGSSREQAPQALRQ
LRVRAVVAPSFGGLFYRNAINLGLPVLVCPDTSRLADGMLATLDLDRAVLQLSDGTTVACEPVPAFLREILQAGGLIPHL
KAKLASR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=98, Percent_Identity=42.8571428571429, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17842; Mature: 17711

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPSVPASAGRIWRFGDNIDTDAMAPAVWMKAAPEALAAHCLKSLRPEFPDQMQPGDVV
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEE
VAGANFGIGSSREQAPQALRQLRVRAVVAPSFGGLFYRNAINLGLPVLVCPDTSRLADGM
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEECCCCHHHHCCH
LATLDLDRAVLQLSDGTTVACEPVPAFLREILQAGGLIPHLKAKLASR
HHHHHHHHHHEEECCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
TEPSVPASAGRIWRFGDNIDTDAMAPAVWMKAAPEALAAHCLKSLRPEFPDQMQPGDVV
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEE
VAGANFGIGSSREQAPQALRQLRVRAVVAPSFGGLFYRNAINLGLPVLVCPDTSRLADGM
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEECCCCHHHHCCH
LATLDLDRAVLQLSDGTTVACEPVPAFLREILQAGGLIPHLKAKLASR
HHHHHHHHHHEEECCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA