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The map label for this gene is mdh [H]

Identifier: 73539123

GI number: 73539123

Start: 2060143

End: 2061201

Strand: Direct

Name: mdh [H]

Synonym: Reut_B5300

Alternate gene names: 73539123

Gene position: 2060143-2061201 (Clockwise)

Preceding gene: 73539122

Following gene: 73539124

Centisome position: 75.57

GC content: 69.78

Gene sequence:

>1059_bases
ATGACGACCCGATGGAATGTCCAAGGCCTGGAGCAATGGACGTCGGCCGTATTCGAATCGTGCGGCGTGGCGCCCGAGCA
TGCCGTCGAGGCCGCCGCCGCGCTCGTGCGCAGCGAACTGCGCGGCTACAAGACGCATGGCATGACACGCGTGCCGTCTT
ACGTCGACCGCCTGCGCGCCGGTGACTTCAATCCGCGCGCGACAATGTCGCACCGTGCCTTTGCCGGCGGCATCGTGCTG
GACGCCGATGGTGCCATGGGACAGGTTGCGGGGCCGCATGCGGTTCGGCTTGGTCTGGAAGCACTGGAATCGAGCGCTTC
CGTGCTCGTCGCGGTGCAGTCGTGCGGCCACCTTGGCGCACTTGGCATCCATGCACTGCTCGCGGCCGAGGCCGGCGCGT
TCTGCATGGTGGGACAGCGCACCCCGCCGGTGCTCGGCATGGAAGGCTTTTCCGGCCCCGCCATCGGTCACAACCCGATC
GCCTTCGGTTGCCCGCTGCCGGGCCAGGCGCCGATCGTGTTCGATGTGGCGTGCAGCGTCGCCGCGCGCGGTCACATCCT
GCTTGCCGCCCGCGAGGGCAAACCCATTCCCGAAGGCTGGGCGCTCGACGCCGACGGTCAGCCGACCACCGACGCGCAAC
GCGCACTCGCCGGGTCGCTGTTACCCACGGGCGGCCACAAGGGCATCGGCATCGCCATGATGGTGGAGTGCCTCGCGGGT
GCGCTGGCCGCCACGGCCGATTCGCTCTCGCCAGAGCGCAACCACGTCGGCAGCGGCGGTGCGGTCGGCCGTCAGGGCGG
CTTTGTCTGGCTGGTCAAGCCCGAGGCCTTTTCCGGCCAGGGCGTCTTCGCCGACTACATGGCGCAGTGGACCGGCAACT
ACCTGGCCGCCGGCGGCGGGGATGCACGGCTCCCCGGCCACCGCGGCGACACGCTGGAACGCGAAGGCCGAGCGCACGGC
ATCGCACTGCCAGACGCCATTGCCCGTGAGCTAGGCGCGCTGGGCCAGCAACTGGGCATTCCGCTTCCAGTCCCGCATCC
AGTCTCGCTTCCGGCCTGA

Upstream 100 bases:

>100_bases
AAATGCGCGGCTACACGCTGCGCGACCAGCCGCTCTACTACCAGTCGGTCTATGCCGCGCCGTTCACCGAGCGGCTGATG
ATCGTGCGGGAGAAATCGTC

Downstream 100 bases:

>100_bases
TCCAACCACACACAGATTGCTGATATCCGGGCTACCGGCAGACGCTGGCCGTCCCTACCCTACAAACACACTGGCTGAAT
CCGCCCCATGAACGCTCCAA

Product: malate/L-lactate dehydrogenase

Products: NA

Alternate protein names: (R)-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTTRWNVQGLEQWTSAVFESCGVAPEHAVEAAAALVRSELRGYKTHGMTRVPSYVDRLRAGDFNPRATMSHRAFAGGIVL
DADGAMGQVAGPHAVRLGLEALESSASVLVAVQSCGHLGALGIHALLAAEAGAFCMVGQRTPPVLGMEGFSGPAIGHNPI
AFGCPLPGQAPIVFDVACSVAARGHILLAAREGKPIPEGWALDADGQPTTDAQRALAGSLLPTGGHKGIGIAMMVECLAG
ALAATADSLSPERNHVGSGGAVGRQGGFVWLVKPEAFSGQGVFADYMAQWTGNYLAAGGGDARLPGHRGDTLEREGRAHG
IALPDAIARELGALGQQLGIPLPVPHPVSLPA

Sequences:

>Translated_352_residues
MTTRWNVQGLEQWTSAVFESCGVAPEHAVEAAAALVRSELRGYKTHGMTRVPSYVDRLRAGDFNPRATMSHRAFAGGIVL
DADGAMGQVAGPHAVRLGLEALESSASVLVAVQSCGHLGALGIHALLAAEAGAFCMVGQRTPPVLGMEGFSGPAIGHNPI
AFGCPLPGQAPIVFDVACSVAARGHILLAAREGKPIPEGWALDADGQPTTDAQRALAGSLLPTGGHKGIGIAMMVECLAG
ALAATADSLSPERNHVGSGGAVGRQGGFVWLVKPEAFSGQGVFADYMAQWTGNYLAAGGGDARLPGHRGDTLEREGRAHG
IALPDAIARELGALGQQLGIPLPVPHPVSLPA
>Mature_351_residues
TTRWNVQGLEQWTSAVFESCGVAPEHAVEAAAALVRSELRGYKTHGMTRVPSYVDRLRAGDFNPRATMSHRAFAGGIVLD
ADGAMGQVAGPHAVRLGLEALESSASVLVAVQSCGHLGALGIHALLAAEAGAFCMVGQRTPPVLGMEGFSGPAIGHNPIA
FGCPLPGQAPIVFDVACSVAARGHILLAAREGKPIPEGWALDADGQPTTDAQRALAGSLLPTGGHKGIGIAMMVECLAGA
LAATADSLSPERNHVGSGGAVGRQGGFVWLVKPEAFSGQGVFADYMAQWTGNYLAAGGGDARLPGHRGDTLEREGRAHGI
ALPDAIARELGALGQQLGIPLPVPHPVSLPA

Specific function: Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786727, Length=339, Percent_Identity=29.2035398230088, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787020, Length=347, Percent_Identity=28.5302593659942, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790000, Length=324, Percent_Identity=24.6913580246914, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536633, Length=245, Percent_Identity=28.1632653061224, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17507179, Length=248, Percent_Identity=27.8225806451613, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667904, Length=313, Percent_Identity=26.8370607028754, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45553211, Length=313, Percent_Identity=26.8370607028754, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667912, Length=313, Percent_Identity=26.8370607028754, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667908, Length=313, Percent_Identity=26.8370607028754, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24658010, Length=330, Percent_Identity=25.4545454545455, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.272; =1.1.1.37; =1.1.1.82 [H]

Molecular weight: Translated: 36018; Mature: 35887

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRWNVQGLEQWTSAVFESCGVAPEHAVEAAAALVRSELRGYKTHGMTRVPSYVDRLRA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GDFNPRATMSHRAFAGGIVLDADGAMGQVAGPHAVRLGLEALESSASVLVAVQSCGHLGA
CCCCCCCCCCCHHEECCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHCCCHHH
LGIHALLAAEAGAFCMVGQRTPPVLGMEGFSGPAIGHNPIAFGCPLPGQAPIVFDVACSV
HHHHHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEHHHH
AARGHILLAAREGKPIPEGWALDADGQPTTDAQRALAGSLLPTGGHKGIGIAMMVECLAG
HCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALAATADSLSPERNHVGSGGAVGRQGGFVWLVKPEAFSGQGVFADYMAQWTGNYLAAGGG
HHHHHHHCCCCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCC
DARLPGHRGDTLEREGRAHGIALPDAIARELGALGQQLGIPLPVPHPVSLPA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTRWNVQGLEQWTSAVFESCGVAPEHAVEAAAALVRSELRGYKTHGMTRVPSYVDRLRA
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GDFNPRATMSHRAFAGGIVLDADGAMGQVAGPHAVRLGLEALESSASVLVAVQSCGHLGA
CCCCCCCCCCCHHEECCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHCCCHHH
LGIHALLAAEAGAFCMVGQRTPPVLGMEGFSGPAIGHNPIAFGCPLPGQAPIVFDVACSV
HHHHHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEHHHH
AARGHILLAAREGKPIPEGWALDADGQPTTDAQRALAGSLLPTGGHKGIGIAMMVECLAG
HCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALAATADSLSPERNHVGSGGAVGRQGGFVWLVKPEAFSGQGVFADYMAQWTGNYLAAGGG
HHHHHHHCCCCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCC
DARLPGHRGDTLEREGRAHGIALPDAIARELGALGQQLGIPLPVPHPVSLPA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2110059; 10850983 [H]