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The map label for this gene is oprM [H]

Identifier: 73539060

GI number: 73539060

Start: 1988309

End: 1989859

Strand: Direct

Name: oprM [H]

Synonym: Reut_B5237

Alternate gene names: 73539060

Gene position: 1988309-1989859 (Clockwise)

Preceding gene: 73539059

Following gene: 73539063

Centisome position: 72.93

GC content: 66.6

Gene sequence:

>1551_bases
ATGAGCCACATGAAAGCACAGCAAACGCGAACCTTCCCTCCGCTCCTGCGTATCGCGACAGTTGCAATTCCGCTGGCGCT
GACAGCCTGCACGCTGGAGCCCACGTACGAGCGTCCCGACCAGCCGGTGCCCAACGCCTGGCCAACCGGAGCGGCCTATG
TGCAGGGCACAGGCGTGCAGGCACAAGCGCAGACGCCGGCTGCGAGCCTCGGATGGGAGGACTTCTTCACCGATCCGGGG
CTGCGCCGGCTGATCGAACTGGCGCTGGCCAATAACCGCGACCTGCGCATGGCGACGCTGGCCATCGACCAGGCGCGCGC
GCAGTACCGCATCCAGCGGGCCGCGCAGTTCCCGTCCCTCGATGCCAACGCGAGCATGGTTGCGACGCGCATACCCGGCG
CGCTGATCGGCCAGGGGGCGCAGTCATCGGTCATCCATGCCTATGCGGCTGGCGTCGGCTTCAATGCCTTTGAGCTCGAT
GTGTTTGGCCGTGTGCGAAGCCTCAAGCATGAGGCGCTGGAACAGTACCTGGCTGTGCAGGAGGTGCAACGCAGCGCGCA
GATCAGCCTGGTGGCGGAGGTTGCAAGCGCCTACCTGACGCTGCAGGCGGACCGGGCGCTGCTGAAGATCTCGCAGGACA
CGCTGAAGGTCCAGCAAGACACCGTGCAGATGGTCGAGCGCAGCAAGCGCGCAGGCGGCATGGCGCAGATCGACATGCAT
CGCGCGCAGACGCAACTCGAAACCGCACGCGTCGGCGTCGAACAGTACACACGTCAGGTCGCGCAGGACGAAAATGCGCT
GACGCTGCTTGTAGGTACGCAGTTGCCGGCGGACCTGCCGGCGCCGCAACCGTTCGAGAACACGTCGTTCGTCGCCGAAC
TGCCGCCTGGCCTGCCCTCGGCGCTGCTTGAGCAACGGCCTGACATCATGGCCGCAGAGCATCGGCTGAAGGCGGCAAAC
GCCGACATCGGCGCGGCGCGCGCAGCCTTCTTCCCAAGCATCTCGCTAACCGCGGCGATCGGCGTCGCGAGCACCGCGCT
GGCCGGGCTGTTTTCAAGCGGCATGGCGTGGGCGTTCGCGCCCCAGATCACCATGCCGATCTTCCATGGCGGGGCTAACC
AGGCCAGGCTGGATGCGTCGAAGGTACAGAAGGACATCAACGTCGCCGCCTACGAAAAGACTATCCAGCAGGCGTTCCGC
GAAGTGGCGGACGGCCTCGCAGCGCGGGGCACCTATGACCGCCAGGCGGCAGCGCAGGAAACCCTCTCGCGCGAGATAGA
GGAAACGCGGCGCCTCTCCGAGATGCGGTTTCGCAACGGCGTGGACAACTACTTCCCGGTCTTCGACGCGCAGCGCCAGC
TTTATTCGGCGCAGCAGACGCTCGTCACCATCCGGCTCGCACGGCTGACGAGCCGCGTCGATCTTTACAAGGCGCTCGGC
GGTGGCTGGTCCCAGCATACAGCCATGGCAGAGGCCCCGGCCGCGCAATCTGCAGCGCCCGGCGCCCAGGCCGCCAGCGC
CTCGCTCCGGACCGGAACGGCTTCCCGCTAA

Upstream 100 bases:

>100_bases
TCAGCGAGCCCGCCCACAACATTGCCAATCAGGGCAGGCCGCGCGTCGCCATCGCACAGTTGCTGCGCCGCGCGTTTGCC
GGCTGAAGCGGATAACACGG

Downstream 100 bases:

>100_bases
GAAGTCCACGCGGCGAATGCAAAACGGGCACCGGCGATGCTTCGCCGGTGCCCGCCATGACAACGTCCGCTGTATCGCCA
GCTCAAGCCATCTCAATAGC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSHMKAQQTRTFPPLLRIATVAIPLALTACTLEPTYERPDQPVPNAWPTGAAYVQGTGVQAQAQTPAASLGWEDFFTDPG
LRRLIELALANNRDLRMATLAIDQARAQYRIQRAAQFPSLDANASMVATRIPGALIGQGAQSSVIHAYAAGVGFNAFELD
VFGRVRSLKHEALEQYLAVQEVQRSAQISLVAEVASAYLTLQADRALLKISQDTLKVQQDTVQMVERSKRAGGMAQIDMH
RAQTQLETARVGVEQYTRQVAQDENALTLLVGTQLPADLPAPQPFENTSFVAELPPGLPSALLEQRPDIMAAEHRLKAAN
ADIGAARAAFFPSISLTAAIGVASTALAGLFSSGMAWAFAPQITMPIFHGGANQARLDASKVQKDINVAAYEKTIQQAFR
EVADGLAARGTYDRQAAAQETLSREIEETRRLSEMRFRNGVDNYFPVFDAQRQLYSAQQTLVTIRLARLTSRVDLYKALG
GGWSQHTAMAEAPAAQSAAPGAQAASASLRTGTASR

Sequences:

>Translated_516_residues
MSHMKAQQTRTFPPLLRIATVAIPLALTACTLEPTYERPDQPVPNAWPTGAAYVQGTGVQAQAQTPAASLGWEDFFTDPG
LRRLIELALANNRDLRMATLAIDQARAQYRIQRAAQFPSLDANASMVATRIPGALIGQGAQSSVIHAYAAGVGFNAFELD
VFGRVRSLKHEALEQYLAVQEVQRSAQISLVAEVASAYLTLQADRALLKISQDTLKVQQDTVQMVERSKRAGGMAQIDMH
RAQTQLETARVGVEQYTRQVAQDENALTLLVGTQLPADLPAPQPFENTSFVAELPPGLPSALLEQRPDIMAAEHRLKAAN
ADIGAARAAFFPSISLTAAIGVASTALAGLFSSGMAWAFAPQITMPIFHGGANQARLDASKVQKDINVAAYEKTIQQAFR
EVADGLAARGTYDRQAAAQETLSREIEETRRLSEMRFRNGVDNYFPVFDAQRQLYSAQQTLVTIRLARLTSRVDLYKALG
GGWSQHTAMAEAPAAQSAAPGAQAASASLRTGTASR
>Mature_515_residues
SHMKAQQTRTFPPLLRIATVAIPLALTACTLEPTYERPDQPVPNAWPTGAAYVQGTGVQAQAQTPAASLGWEDFFTDPGL
RRLIELALANNRDLRMATLAIDQARAQYRIQRAAQFPSLDANASMVATRIPGALIGQGAQSSVIHAYAAGVGFNAFELDV
FGRVRSLKHEALEQYLAVQEVQRSAQISLVAEVASAYLTLQADRALLKISQDTLKVQQDTVQMVERSKRAGGMAQIDMHR
AQTQLETARVGVEQYTRQVAQDENALTLLVGTQLPADLPAPQPFENTSFVAELPPGLPSALLEQRPDIMAAEHRLKAANA
DIGAARAAFFPSISLTAAIGVASTALAGLFSSGMAWAFAPQITMPIFHGGANQARLDASKVQKDINVAAYEKTIQQAFRE
VADGLAARGTYDRQAAAQETLSREIEETRRLSEMRFRNGVDNYFPVFDAQRQLYSAQQTLVTIRLARLTSRVDLYKALGG
GWSQHTAMAEAPAAQSAAPGAQAASASLRTGTASR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=470, Percent_Identity=40.6382978723404, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI2367348, Length=422, Percent_Identity=24.4075829383886, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55655; Mature: 55523

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHMKAQQTRTFPPLLRIATVAIPLALTACTLEPTYERPDQPVPNAWPTGAAYVQGTGVQ
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEECCCCE
AQAQTPAASLGWEDFFTDPGLRRLIELALANNRDLRMATLAIDQARAQYRIQRAAQFPSL
ECCCCCCHHCCCHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCC
DANASMVATRIPGALIGQGAQSSVIHAYAAGVGFNAFELDVFGRVRSLKHEALEQYLAVQ
CCCCHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EVQRSAQISLVAEVASAYLTLQADRALLKISQDTLKVQQDTVQMVERSKRAGGMAQIDMH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RAQTQLETARVGVEQYTRQVAQDENALTLLVGTQLPADLPAPQPFENTSFVAELPPGLPS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHH
ALLEQRPDIMAAEHRLKAANADIGAARAAFFPSISLTAAIGVASTALAGLFSSGMAWAFA
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
PQITMPIFHGGANQARLDASKVQKDINVAAYEKTIQQAFREVADGLAARGTYDRQAAAQE
CCEECEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TLSREIEETRRLSEMRFRNGVDNYFPVFDAQRQLYSAQQTLVTIRLARLTSRVDLYKALG
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGWSQHTAMAEAPAAQSAAPGAQAASASLRTGTASR
CCCHHHHHHHHCCCCCCCCCCCHHHHCHHCCCCCCC
>Mature Secondary Structure 
SHMKAQQTRTFPPLLRIATVAIPLALTACTLEPTYERPDQPVPNAWPTGAAYVQGTGVQ
CCCCCHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEECCCCE
AQAQTPAASLGWEDFFTDPGLRRLIELALANNRDLRMATLAIDQARAQYRIQRAAQFPSL
ECCCCCCHHCCCHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCC
DANASMVATRIPGALIGQGAQSSVIHAYAAGVGFNAFELDVFGRVRSLKHEALEQYLAVQ
CCCCHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EVQRSAQISLVAEVASAYLTLQADRALLKISQDTLKVQQDTVQMVERSKRAGGMAQIDMH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RAQTQLETARVGVEQYTRQVAQDENALTLLVGTQLPADLPAPQPFENTSFVAELPPGLPS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHH
ALLEQRPDIMAAEHRLKAANADIGAARAAFFPSISLTAAIGVASTALAGLFSSGMAWAFA
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
PQITMPIFHGGANQARLDASKVQKDINVAAYEKTIQQAFREVADGLAARGTYDRQAAAQE
CCEECEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TLSREIEETRRLSEMRFRNGVDNYFPVFDAQRQLYSAQQTLVTIRLARLTSRVDLYKALG
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGWSQHTAMAEAPAAQSAAPGAQAASASLRTGTASR
CCCHHHHHHHHCCCCCCCCCCCHHHHCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]