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The map label for this gene is cspA [H]

Identifier: 73539008

GI number: 73539008

Start: 1926286

End: 1926489

Strand: Reverse

Name: cspA [H]

Synonym: Reut_B5185

Alternate gene names: 73539008

Gene position: 1926489-1926286 (Counterclockwise)

Preceding gene: 73539015

Following gene: 73539005

Centisome position: 70.67

GC content: 51.96

Gene sequence:

>204_bases
GTGGAAACCGGTACAGTAAAGTGGTTTAATGATTCAAAGGGTTTTGGCTTCATTACGCCTGATGCAGGCGGAAACGATCT
GTTCGCCCATTTCTCCGAAATTCAGGGGAACGGGTTCAAGTCGCTGCAGGAAGGCCAGAAAGTGCGGTATGTGGCTGGTG
TAGGCCAGAAGGGTCCGGCTGCGACCAAGATCGAACCGATCTAA

Upstream 100 bases:

>100_bases
ACGCCCCGGCGGCGCCACTTTCTCTCCATGTTTCCTCCTTGATCGTCATGCCTCGCTGCCCACATTTTCAGATCGGCGAG
AATTCTTAAGGAGTCCTTAC

Downstream 100 bases:

>100_bases
GTCGACCGCCCAAGGTACGCAAGAAGCCCCGTTGGGAAAGCCGGCGGGGCTTTTCCTTTATGGGTTCACCGTGATGGGTA
CGGAAGGAGTAGCGTCGGGA

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
METGTVKWFNDSKGFGFITPDAGGNDLFAHFSEIQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI

Sequences:

>Translated_67_residues
METGTVKWFNDSKGFGFITPDAGGNDLFAHFSEIQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI
>Mature_67_residues
METGTVKWFNDSKGFGFITPDAGGNDLFAHFSEIQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=64.0625, Blast_Score=85, Evalue=8e-19,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=60, Blast_Score=83, Evalue=3e-18,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=60.655737704918, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=58.0645161290323, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=53.7313432835821, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=58.0645161290323, Blast_Score=75, Evalue=8e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=54.8387096774194, Blast_Score=73, Evalue=3e-15,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7176; Mature: 7176

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGTVKWFNDSKGFGFITPDAGGNDLFAHFSEIQGNGFKSLQEGQKVRYVAGVGQKGPA
CCCCCEEEEECCCCCEEECCCCCCCCHHEEHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
ATKIEPI
CCEECCC
>Mature Secondary Structure
METGTVKWFNDSKGFGFITPDAGGNDLFAHFSEIQGNGFKSLQEGQKVRYVAGVGQKGPA
CCCCCEEEEECCCCCEEECCCCCCCCHHEEHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
ATKIEPI
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]