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The map label for this gene is purA2

Identifier: 73539003

GI number: 73539003

Start: 1921281

End: 1922639

Strand: Reverse

Name: purA2

Synonym: Reut_B5180

Alternate gene names: 73539003

Gene position: 1922639-1921281 (Counterclockwise)

Preceding gene: 73539004

Following gene: 73539002

Centisome position: 70.53

GC content: 63.8

Gene sequence:

>1359_bases
ATGGACACCGCGACAAAAACCGGGTATGCGGACGTGCTGGTTGGGCTGCAGTACGGCGACGAGGGAAAGGCCCGGGTGGT
TGACCACCTCGCTGCCGGGTATGACGTGATTGCACGCTTCAACGGCGGCGCGAACGCAGGGCACACCATTGCCACGTCTG
AGGGCATCTTGCGGCTCCGTCAGGTACCTTCTGGCGTGCTGCACCCTCGGGTTTCCCTCTACATCGGCTCGGGCTGCGTG
ATCGGCTTGCGGCAACTGGCATCCGAAATCGAAATGTTGGCCGGGCAGGGAATCGACCTGGCGGGGCGCCTGACCATCAG
TGACCGATGTCCGGTCGTGCAGCCAGCGCACTTCCTGTCTGATCGTCGGGATGGCGGGCGCATCGGCACCACCGGCAACG
GTATCGGACCCTGTTACGCGGACTTGGCGGCCCGCATGCGGGGCGGCGAGCGATCTGCGTTTCAGCTTTGCGACCTCATG
CGGGACGAGAGCAGGGTGTTCGAACAGATGATGCGGCTGGCAGCCCAACGCGATGACGAGGATATCGCGGTCCTCATTCA
AGAGATGCGCCAGGCATGGCAGGTGGTGCGGCCCTTCGTAACCGATAATCCGGTTGCCCTCCTGGAGCGGGTTGAAGGCG
GGGCGCGGGTTCTGTTCGAGGGCGCTCAGTCAGTCATGCTGGATGTTGTGCAGGGCGCCCAACCCTGGGTGACGTCCAGC
CATACCCTGCCTTCCTACGCCTACGTGGGCGGTGACCTCCCATGCCGGTATCACCGCAAGACAATTGGCGTGGCCAAGGC
CATGGTCTCCCGCGTTGGCAGCGGTCCCTTGCCGACGGAACTCGGCGCGGAGCGCTCAGAGGCATATTGCGCCGACGCGG
GGCGCGAGGGGCGCGGCAGAGCTGATGAGGCGGCACGCTTTGATCCGCGGGCATTGCTCGCGCAAGGGGACGCCTTCTCC
ACCGGTATAGCGATCCGCATGCTGTCGAACGAATATGGCACCGGTACCGGCAGGCCGCGTCGGGTAGGTCTGCTCGACGT
CGCTCAACTCCAGCTTGCCATACGGCAGTTTGGGGTCGACGAGGTCTACCTGAACAAGTGCGACAGCCTGGCGGTTTTTG
CGCAGACTCGCGACCGGTGCATTCCGGTCGTTGTCGGCTCACGCGACGGCAATGCCATGCATGTGATGCGATTTCCGGCT
TTCGATGAGAGCGTCATTCCGCGCGACGACGCAACGCCGCTTCCTCCGCAACTTGAAACCCTGCTCGAATGGTTGGCGGA
TGTCCTCGGCCGGCCACTACGCGGCATCGGATTGGGTCCGCAACGTGCGCAGATGCGGCTATTCAAGACTCAACCATGA

Upstream 100 bases:

>100_bases
AGAGCGGGGGCGTCATGCACGCCTGGCGATAGGCGTGGCGAGCCTTCCCTTCGGTGCGCCGGTGGAAATCGAAGCGACCT
TTGCACTGGACGTGTAGTCG

Downstream 100 bases:

>100_bases
ACCCCATGAACATTGAGACACCATCCTACTTTCGTAGCGAAATGCGTCGCGCCGACTTGGCCTGGACCGACTGGGCTGCC
GTCGAAGCCTTCCTCAGGGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDTATKTGYADVLVGLQYGDEGKARVVDHLAAGYDVIARFNGGANAGHTIATSEGILRLRQVPSGVLHPRVSLYIGSGCV
IGLRQLASEIEMLAGQGIDLAGRLTISDRCPVVQPAHFLSDRRDGGRIGTTGNGIGPCYADLAARMRGGERSAFQLCDLM
RDESRVFEQMMRLAAQRDDEDIAVLIQEMRQAWQVVRPFVTDNPVALLERVEGGARVLFEGAQSVMLDVVQGAQPWVTSS
HTLPSYAYVGGDLPCRYHRKTIGVAKAMVSRVGSGPLPTELGAERSEAYCADAGREGRGRADEAARFDPRALLAQGDAFS
TGIAIRMLSNEYGTGTGRPRRVGLLDVAQLQLAIRQFGVDEVYLNKCDSLAVFAQTRDRCIPVVVGSRDGNAMHVMRFPA
FDESVIPRDDATPLPPQLETLLEWLADVLGRPLRGIGLGPQRAQMRLFKTQP

Sequences:

>Translated_452_residues
MDTATKTGYADVLVGLQYGDEGKARVVDHLAAGYDVIARFNGGANAGHTIATSEGILRLRQVPSGVLHPRVSLYIGSGCV
IGLRQLASEIEMLAGQGIDLAGRLTISDRCPVVQPAHFLSDRRDGGRIGTTGNGIGPCYADLAARMRGGERSAFQLCDLM
RDESRVFEQMMRLAAQRDDEDIAVLIQEMRQAWQVVRPFVTDNPVALLERVEGGARVLFEGAQSVMLDVVQGAQPWVTSS
HTLPSYAYVGGDLPCRYHRKTIGVAKAMVSRVGSGPLPTELGAERSEAYCADAGREGRGRADEAARFDPRALLAQGDAFS
TGIAIRMLSNEYGTGTGRPRRVGLLDVAQLQLAIRQFGVDEVYLNKCDSLAVFAQTRDRCIPVVVGSRDGNAMHVMRFPA
FDESVIPRDDATPLPPQLETLLEWLADVLGRPLRGIGLGPQRAQMRLFKTQP
>Mature_452_residues
MDTATKTGYADVLVGLQYGDEGKARVVDHLAAGYDVIARFNGGANAGHTIATSEGILRLRQVPSGVLHPRVSLYIGSGCV
IGLRQLASEIEMLAGQGIDLAGRLTISDRCPVVQPAHFLSDRRDGGRIGTTGNGIGPCYADLAARMRGGERSAFQLCDLM
RDESRVFEQMMRLAAQRDDEDIAVLIQEMRQAWQVVRPFVTDNPVALLERVEGGARVLFEGAQSVMLDVVQGAQPWVTSS
HTLPSYAYVGGDLPCRYHRKTIGVAKAMVSRVGSGPLPTELGAERSEAYCADAGREGRGRADEAARFDPRALLAQGDAFS
TGIAIRMLSNEYGTGTGRPRRVGLLDVAQLQLAIRQFGVDEVYLNKCDSLAVFAQTRDRCIPVVVGSRDGNAMHVMRFPA
FDESVIPRDDATPLPPQLETLLEWLADVLGRPLRGIGLGPQRAQMRLFKTQP

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=467, Percent_Identity=30.6209850107066, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI22748717, Length=471, Percent_Identity=29.723991507431, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI40316944, Length=441, Percent_Identity=28.1179138321995, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1790620, Length=463, Percent_Identity=30.4535637149028, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146553, Length=483, Percent_Identity=28.3643892339545, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146555, Length=483, Percent_Identity=28.3643892339545, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324109, Length=473, Percent_Identity=28.9640591966173, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21356033, Length=465, Percent_Identity=28.3870967741935, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA2_CUPPJ (Q46QQ8)

Other databases:

- EMBL:   CP000091
- RefSeq:   YP_299370.1
- ProteinModelPortal:   Q46QQ8
- SMR:   Q46QQ8
- GeneID:   3614614
- GenomeReviews:   CP000091_GR
- KEGG:   reu:Reut_B5180
- NMPDR:   fig|264198.3.peg.6155
- HOGENOM:   HBG658237
- OMA:   LFEGAQS
- ProtClustDB:   CLSK939171
- BioCyc:   REUT264198:REUT_B5180-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48973; Mature: 48973

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 20-20 ACT_SITE 48-48 BINDING 131-131 BINDING 145-145 BINDING 223-223 BINDING 238-238 BINDING 338-338 BINDING 340-340

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTATKTGYADVLVGLQYGDEGKARVVDHLAAGYDVIARFNGGANAGHTIATSEGILRLR
CCCCCCCCHHHEEEEEEECCCCHHHHHHHHHCCHHEEEEECCCCCCCCEEECHHHHHHHH
QVPSGVLHPRVSLYIGSGCVIGLRQLASEIEMLAGQGIDLAGRLTISDRCPVVQPAHFLS
HCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
DRRDGGRIGTTGNGIGPCYADLAARMRGGERSAFQLCDLMRDESRVFEQMMRLAAQRDDE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DIAVLIQEMRQAWQVVRPFVTDNPVALLERVEGGARVLFEGAQSVMLDVVQGAQPWVTSS
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
HTLPSYAYVGGDLPCRYHRKTIGVAKAMVSRVGSGPLPTELGAERSEAYCADAGREGRGR
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHCCCCCCCCC
ADEAARFDPRALLAQGDAFSTGIAIRMLSNEYGTGTGRPRRVGLLDVAQLQLAIRQFGVD
CCHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHCCC
EVYLNKCDSLAVFAQTRDRCIPVVVGSRDGNAMHVMRFPAFDESVIPRDDATPLPPQLET
HHHHHCCCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LLEWLADVLGRPLRGIGLGPQRAQMRLFKTQP
HHHHHHHHHCCCCCCCCCCCHHHHHEEECCCC
>Mature Secondary Structure
MDTATKTGYADVLVGLQYGDEGKARVVDHLAAGYDVIARFNGGANAGHTIATSEGILRLR
CCCCCCCCHHHEEEEEEECCCCHHHHHHHHHCCHHEEEEECCCCCCCCEEECHHHHHHHH
QVPSGVLHPRVSLYIGSGCVIGLRQLASEIEMLAGQGIDLAGRLTISDRCPVVQPAHFLS
HCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
DRRDGGRIGTTGNGIGPCYADLAARMRGGERSAFQLCDLMRDESRVFEQMMRLAAQRDDE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DIAVLIQEMRQAWQVVRPFVTDNPVALLERVEGGARVLFEGAQSVMLDVVQGAQPWVTSS
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
HTLPSYAYVGGDLPCRYHRKTIGVAKAMVSRVGSGPLPTELGAERSEAYCADAGREGRGR
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHCCCCCCCCC
ADEAARFDPRALLAQGDAFSTGIAIRMLSNEYGTGTGRPRRVGLLDVAQLQLAIRQFGVD
CCHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHCCC
EVYLNKCDSLAVFAQTRDRCIPVVVGSRDGNAMHVMRFPAFDESVIPRDDATPLPPQLET
HHHHHCCCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LLEWLADVLGRPLRGIGLGPQRAQMRLFKTQP
HHHHHHHHHCCCCCCCCCCCHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA