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The map label for this gene is gatA2 [H]

Identifier: 73538967

GI number: 73538967

Start: 1882446

End: 1883945

Strand: Direct

Name: gatA2 [H]

Synonym: Reut_B5142

Alternate gene names: 73538967

Gene position: 1882446-1883945 (Clockwise)

Preceding gene: 73538966

Following gene: 73538968

Centisome position: 69.05

GC content: 65.8

Gene sequence:

>1500_bases
ATGAAATCAACCACCGACTCCGGCACCCGGGTCATGGAGGCCACCGTAGAGGGCGTCCATGCCGCCATGCGTGAAGGCCG
ACTCACGGCGCGTCAACTGGTGCAGCGATACCTGGACCGGATCGCCGCCTATGACAGGAACGGCCCGGGCATCTACAGCG
TACTGCAGTTCAATCCCCACGCATTGTCAGAAGCGGATCAACTGGACGCGCAAGCTGCGGCCGCGCCATCCGTTCCGCTC
GCGCGTCTGCATGGCATACCGGTCCTGGTCAAGGACAACATCGAATGCGCCGGTATGGAGACGACTGCGGGCGCACGTTG
CCTGCAAGGCAATCTTGCGGTGGAGGACGCCTTCATCGTGCGCAGGCTGCGCGAGGCCGGCGCGATCGTACTGGCCAAGA
CCAACCTGCACGAGCTGGCATCTGGCGGCGAGACCGTCAGCACGCTGGGCGGACAAACCCGCAACCCCTATGACCTCACG
CGGACGCCGGGTGGTTCAAGCGGCGGCACGGCCGCGGGTATTGCTGCCAACTTCGGCCTGCTGGGAATCGGCACGGATGG
TGTCAATTCCATTCGCTCCCCCGCTTCGGCGAACAACCTCGTCGGCTTGCGTCCGACGATGGGACTGGTCAGCCGTACCG
GACTGATCCCTTGCGGGCTGACGCAAGACACCATCGGGCCGATCACACGCACCGTGACCGATGCCGCGCTCTTGCTCGAC
ATCATCGCCGGCTACGACCCGGCGGACCCGGTTACCGGCGAAGCCGCTTCGCATACCCGGCCTAGCTACACCGAAGGACT
GCATCCTGACGCGCTGAAAGGGGCTCGCATCGGCGTACTGCGCTCCTTCTTTGGCGATGGGCCCGAGCATCGCGCCGTCA
ATGCCGTCATGCAGAACGCGCTTGCCACCATCCGTGCACGAGGTGCCGAGCTGGTGCAGATCGATGAAGCGATCCACCCC
GACGCGCTGCTGGCCGACACGCTGGTGCACCTCTACGAAATGAAGGGCGACCTTGACGCCTACCTTGCCGATGCCCCATC
CGACGTGTCGGTACGCTCCATGGAAGACATCATCGCTTCGGCGAGCGTGCATCCCAGCGTCGCGGGAACGCTGAAGAACG
CCATGGCACTGGCAGGCAACGAGACCGAATACCGCGAACGGTTGCAACGCCAGCAAGCGTTGCGTGAACGCGTCCTCGGG
TTGATGGCAGCGCACCGTCTCGATGCGCTGGCGTTTCCGCACCAGCGCCGCCTGGTCGTGCCCGTGGGCGAGGCCCAGGC
CGAGCGCAATGGCGTGCTCGCGTCGGCCACGGGCTTCCCCGCAATCGTCATCCCGGCAGGCTTCTCCGAACCGGATGCCC
ACGCCGCACATGGCGTTCCGGTCGGCCTGGAGTTGTTTGGCCGACCGTTTTCGGAAGCCGTGTTGTTGCGGCTGGCGTTC
GCGGCGGAGCACGCCGTGGGCGCCCGCCGCCCGCCTCTGACTGCGCCAGCACTGACCTAG

Upstream 100 bases:

>100_bases
GCAAAGCTGGTCAACCGGTAGCGCATGTCGGGGAGCGGCGTGCCGCTCCCCGACGACTCATCACCCCGGCGCCCTTCCCG
TGGGCGCAAGGAACACAAAC

Downstream 100 bases:

>100_bases
CACCGCGTCGCAGCCGCACTTTCATGACTGCTCTGTTCCAATGCCGGCCAGCGATGCCGGCAGCAAGTTTGCGGACACAC
CGTAACTGACCTCATTTCGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKSTTDSGTRVMEATVEGVHAAMREGRLTARQLVQRYLDRIAAYDRNGPGIYSVLQFNPHALSEADQLDAQAAAAPSVPL
ARLHGIPVLVKDNIECAGMETTAGARCLQGNLAVEDAFIVRRLREAGAIVLAKTNLHELASGGETVSTLGGQTRNPYDLT
RTPGGSSGGTAAGIAANFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRTGLIPCGLTQDTIGPITRTVTDAALLLD
IIAGYDPADPVTGEAASHTRPSYTEGLHPDALKGARIGVLRSFFGDGPEHRAVNAVMQNALATIRARGAELVQIDEAIHP
DALLADTLVHLYEMKGDLDAYLADAPSDVSVRSMEDIIASASVHPSVAGTLKNAMALAGNETEYRERLQRQQALRERVLG
LMAAHRLDALAFPHQRRLVVPVGEAQAERNGVLASATGFPAIVIPAGFSEPDAHAAHGVPVGLELFGRPFSEAVLLRLAF
AAEHAVGARRPPLTAPALT

Sequences:

>Translated_499_residues
MKSTTDSGTRVMEATVEGVHAAMREGRLTARQLVQRYLDRIAAYDRNGPGIYSVLQFNPHALSEADQLDAQAAAAPSVPL
ARLHGIPVLVKDNIECAGMETTAGARCLQGNLAVEDAFIVRRLREAGAIVLAKTNLHELASGGETVSTLGGQTRNPYDLT
RTPGGSSGGTAAGIAANFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRTGLIPCGLTQDTIGPITRTVTDAALLLD
IIAGYDPADPVTGEAASHTRPSYTEGLHPDALKGARIGVLRSFFGDGPEHRAVNAVMQNALATIRARGAELVQIDEAIHP
DALLADTLVHLYEMKGDLDAYLADAPSDVSVRSMEDIIASASVHPSVAGTLKNAMALAGNETEYRERLQRQQALRERVLG
LMAAHRLDALAFPHQRRLVVPVGEAQAERNGVLASATGFPAIVIPAGFSEPDAHAAHGVPVGLELFGRPFSEAVLLRLAF
AAEHAVGARRPPLTAPALT
>Mature_499_residues
MKSTTDSGTRVMEATVEGVHAAMREGRLTARQLVQRYLDRIAAYDRNGPGIYSVLQFNPHALSEADQLDAQAAAAPSVPL
ARLHGIPVLVKDNIECAGMETTAGARCLQGNLAVEDAFIVRRLREAGAIVLAKTNLHELASGGETVSTLGGQTRNPYDLT
RTPGGSSGGTAAGIAANFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRTGLIPCGLTQDTIGPITRTVTDAALLLD
IIAGYDPADPVTGEAASHTRPSYTEGLHPDALKGARIGVLRSFFGDGPEHRAVNAVMQNALATIRARGAELVQIDEAIHP
DALLADTLVHLYEMKGDLDAYLADAPSDVSVRSMEDIIASASVHPSVAGTLKNAMALAGNETEYRERLQRQQALRERVLG
LMAAHRLDALAFPHQRRLVVPVGEAQAERNGVLASATGFPAIVIPAGFSEPDAHAAHGVPVGLELFGRPFSEAVLLRLAF
AAEHAVGARRPPLTAPALT

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=26.171875, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI195972892, Length=512, Percent_Identity=25.5859375, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=260, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=461, Percent_Identity=24.5119305856833, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=191, Percent_Identity=31.9371727748691, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=386, Percent_Identity=27.4611398963731, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=444, Percent_Identity=26.8018018018018, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=412, Percent_Identity=25, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=290, Percent_Identity=28.2758620689655, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=173, Percent_Identity=31.7919075144509, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=514, Percent_Identity=27.2373540856031, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648435, Length=410, Percent_Identity=30.2439024390244, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24648437, Length=410, Percent_Identity=30.2439024390244, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45550774, Length=410, Percent_Identity=30.2439024390244, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24644968, Length=522, Percent_Identity=26.2452107279693, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648441, Length=346, Percent_Identity=31.5028901734104, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648439, Length=346, Percent_Identity=31.5028901734104, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356731, Length=253, Percent_Identity=32.8063241106719, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=25.3036437246964, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=25.3036437246964, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=25.3036437246964, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=25.3036437246964, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=26.5107212475634, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52323; Mature: 52323

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTTDSGTRVMEATVEGVHAAMREGRLTARQLVQRYLDRIAAYDRNGPGIYSVLQFNPH
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
ALSEADQLDAQAAAAPSVPLARLHGIPVLVKDNIECAGMETTAGARCLQGNLAVEDAFIV
HHCHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCEECCCCCCCCCHHHCCCCCHHHHHHH
RRLREAGAIVLAKTNLHELASGGETVSTLGGQTRNPYDLTRTPGGSSGGTAAGIAANFGL
HHHHHCCEEEEEECCHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCE
LGIGTDGVNSIRSPASANNLVGLRPTMGLVSRTGLIPCGLTQDTIGPITRTVTDAALLLD
EEECCCCHHHHCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IIAGYDPADPVTGEAASHTRPSYTEGLHPDALKGARIGVLRSFFGDGPEHRAVNAVMQNA
HHHCCCCCCCCCCCCCCCCCCCHHCCCCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
LATIRARGAELVQIDEAIHPDALLADTLVHLYEMKGDLDAYLADAPSDVSVRSMEDIIAS
HHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
ASVHPSVAGTLKNAMALAGNETEYRERLQRQQALRERVLGLMAAHRLDALAFPHQRRLVV
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PVGEAQAERNGVLASATGFPAIVIPAGFSEPDAHAAHGVPVGLELFGRPFSEAVLLRLAF
ECCCHHHHHCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHH
AAEHAVGARRPPLTAPALT
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSTTDSGTRVMEATVEGVHAAMREGRLTARQLVQRYLDRIAAYDRNGPGIYSVLQFNPH
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
ALSEADQLDAQAAAAPSVPLARLHGIPVLVKDNIECAGMETTAGARCLQGNLAVEDAFIV
HHCHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCEECCCCCCCCCHHHCCCCCHHHHHHH
RRLREAGAIVLAKTNLHELASGGETVSTLGGQTRNPYDLTRTPGGSSGGTAAGIAANFGL
HHHHHCCEEEEEECCHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCE
LGIGTDGVNSIRSPASANNLVGLRPTMGLVSRTGLIPCGLTQDTIGPITRTVTDAALLLD
EEECCCCHHHHCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IIAGYDPADPVTGEAASHTRPSYTEGLHPDALKGARIGVLRSFFGDGPEHRAVNAVMQNA
HHHCCCCCCCCCCCCCCCCCCCHHCCCCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
LATIRARGAELVQIDEAIHPDALLADTLVHLYEMKGDLDAYLADAPSDVSVRSMEDIIAS
HHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
ASVHPSVAGTLKNAMALAGNETEYRERLQRQQALRERVLGLMAAHRLDALAFPHQRRLVV
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PVGEAQAERNGVLASATGFPAIVIPAGFSEPDAHAAHGVPVGLELFGRPFSEAVLLRLAF
ECCCHHHHHCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHH
AAEHAVGARRPPLTAPALT
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]