Click here to switch to the map view.

The map label for this gene is yafC [H]

Identifier: 73538942

GI number: 73538942

Start: 1858248

End: 1859150

Strand: Direct

Name: yafC [H]

Synonym: Reut_B5117

Alternate gene names: 73538942

Gene position: 1858248-1859150 (Clockwise)

Preceding gene: 73538937

Following gene: 73538943

Centisome position: 68.16

GC content: 64.34

Gene sequence:

>903_bases
ATGAAGACGACCATCGACGAACTGCTGGCCTTTGCGGCTGTCGTGGATACGGGTTCGATCACAGCAGCCGCGGAACAGCT
CGACCTGACGGTATCCGCCACCAGCCGCACGCTGAGCCGGCTCGAAGAAAAGCTGCAGACCACGCTGCTGCGCCGGACCA
CGCGGCGATTGGAGCTGACGGAGGAAGGCGCGGCATTCCTGACCCATGCACGCGCCATTCTCGCCATGGTCGACGACGCG
GAAGAGCAGATGGCGGCACGTCGCATGCGCCCCGTGGGACGCCTGCGCGTCGATGCGGCGACGCCCTTCATGCTGCACGT
GCTTGTGCCACTGGTGGAAGGCTTCCGGGCGCGCTACCCGGACGTGGAACTGGAACTGCACTCGAACGAGGGCATCATCG
ACCTGATCGAGAAGCGGGCCGATGTGGCGTTCCGCATCGGCTCGCTCAAGGACTCGACGCTGCATGCGCGGCCGATCGGC
ACCAGCCGCGTGCGGGTGCTGGCCAGCCCCGATTACCTGAAACGACACGGCACACCGACCAGACCTGCCCAGCTTGCGCA
GCATGCGTTGCTCGGCTTCAGCCAGCCCGATTCGCTCAACGACTGGCCATTGCGCGACGACGACGGCAGCATCGTGCACA
TCAAACCAGCCATTGCGTCATCGAGCGGCGAAACGCTCAGGAGCCTGGCACTGGCCGGCCAGGGGATCGTCTGCCTGTCG
GACTTCATGACACGCGAAGACCGCCACAGTGGCCGGCTGGTGCCACTGTTTGCACGGCAGACGCTAGACGAGCGTCAGCC
GATCAACGCCGTCTACTATCGCAATACGGCATTGGCGTCGCGCATCACCTGCTTCGTCGATCATGTGGTTGAGGTGCTGG
GCAAGCAACCGTTCGACGAATAG

Upstream 100 bases:

>100_bases
TGTGGCAGCAAGAGATAACTTGGAATGGAGCCAGTTTGCCGGCTTTACATTTGCAAATTAAGCCGTGTATAAGTCAAATT
CATTTGATTTGAAATCAACA

Downstream 100 bases:

>100_bases
CCGGCCTGGTAGCGCTCGCCCTGAATTCAACCGCAAGCAGCGGAGTTTCGAGAACGGCCTCGGTCCGTACATTGGCATCA
CACGTCATCACAAAGAGTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKTTIDELLAFAAVVDTGSITAAAEQLDLTVSATSRTLSRLEEKLQTTLLRRTTRRLELTEEGAAFLTHARAILAMVDDA
EEQMAARRMRPVGRLRVDAATPFMLHVLVPLVEGFRARYPDVELELHSNEGIIDLIEKRADVAFRIGSLKDSTLHARPIG
TSRVRVLASPDYLKRHGTPTRPAQLAQHALLGFSQPDSLNDWPLRDDDGSIVHIKPAIASSSGETLRSLALAGQGIVCLS
DFMTREDRHSGRLVPLFARQTLDERQPINAVYYRNTALASRITCFVDHVVEVLGKQPFDE

Sequences:

>Translated_300_residues
MKTTIDELLAFAAVVDTGSITAAAEQLDLTVSATSRTLSRLEEKLQTTLLRRTTRRLELTEEGAAFLTHARAILAMVDDA
EEQMAARRMRPVGRLRVDAATPFMLHVLVPLVEGFRARYPDVELELHSNEGIIDLIEKRADVAFRIGSLKDSTLHARPIG
TSRVRVLASPDYLKRHGTPTRPAQLAQHALLGFSQPDSLNDWPLRDDDGSIVHIKPAIASSSGETLRSLALAGQGIVCLS
DFMTREDRHSGRLVPLFARQTLDERQPINAVYYRNTALASRITCFVDHVVEVLGKQPFDE
>Mature_300_residues
MKTTIDELLAFAAVVDTGSITAAAEQLDLTVSATSRTLSRLEEKLQTTLLRRTTRRLELTEEGAAFLTHARAILAMVDDA
EEQMAARRMRPVGRLRVDAATPFMLHVLVPLVEGFRARYPDVELELHSNEGIIDLIEKRADVAFRIGSLKDSTLHARPIG
TSRVRVLASPDYLKRHGTPTRPAQLAQHALLGFSQPDSLNDWPLRDDDGSIVHIKPAIASSSGETLRSLALAGQGIVCLS
DFMTREDRHSGRLVPLFARQTLDERQPINAVYYRNTALASRITCFVDHVVEVLGKQPFDE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=46.8013468013468, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=34.2560553633218, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=28.2828282828283, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=29.8611111111111, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787128, Length=301, Percent_Identity=26.578073089701, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787589, Length=203, Percent_Identity=31.5270935960591, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=26.1016949152542, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=28.90625, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787879, Length=275, Percent_Identity=28.7272727272727, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788297, Length=137, Percent_Identity=32.8467153284672, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786508, Length=281, Percent_Identity=27.7580071174377, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=274, Percent_Identity=24.8175182481752, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=164, Percent_Identity=27.4390243902439, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786448, Length=265, Percent_Identity=26.0377358490566, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=209, Percent_Identity=28.2296650717703, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=275, Percent_Identity=25.4545454545455, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33254; Mature: 33254

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTIDELLAFAAVVDTGSITAAAEQLDLTVSATSRTLSRLEEKLQTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EEGAAFLTHARAILAMVDDAEEQMAARRMRPVGRLRVDAATPFMLHVLVPLVEGFRARYP
CCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
DVELELHSNEGIIDLIEKRADVAFRIGSLKDSTLHARPIGTSRVRVLASPDYLKRHGTPT
CEEEEEECCCCHHHHHHHHHHHEEEECCCCCCCEEECCCCCCCEEEEECCHHHHHCCCCC
RPAQLAQHALLGFSQPDSLNDWPLRDDDGSIVHIKPAIASSSGETLRSLALAGQGIVCLS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
DFMTREDRHSGRLVPLFARQTLDERQPINAVYYRNTALASRITCFVDHVVEVLGKQPFDE
HHHHHHHCCCCCEEEHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTTIDELLAFAAVVDTGSITAAAEQLDLTVSATSRTLSRLEEKLQTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EEGAAFLTHARAILAMVDDAEEQMAARRMRPVGRLRVDAATPFMLHVLVPLVEGFRARYP
CCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
DVELELHSNEGIIDLIEKRADVAFRIGSLKDSTLHARPIGTSRVRVLASPDYLKRHGTPT
CEEEEEECCCCHHHHHHHHHHHEEEECCCCCCCEEECCCCCCCEEEEECCHHHHHCCCCC
RPAQLAQHALLGFSQPDSLNDWPLRDDDGSIVHIKPAIASSSGETLRSLALAGQGIVCLS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
DFMTREDRHSGRLVPLFARQTLDERQPINAVYYRNTALASRITCFVDHVVEVLGKQPFDE
HHHHHHHCCCCCEEEHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]