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The map label for this gene is alkB [H]

Identifier: 73538928

GI number: 73538928

Start: 1845560

End: 1846231

Strand: Direct

Name: alkB [H]

Synonym: Reut_B5103

Alternate gene names: 73538928

Gene position: 1845560-1846231 (Clockwise)

Preceding gene: 73538927

Following gene: 73538930

Centisome position: 67.7

GC content: 68.01

Gene sequence:

>672_bases
ATGACGTTCGACCTCTTCGACAATCTCGACAACTCGCAAGACGGGTATGCCCCCGGCACCGCGCCCGAGCCGCTTGCGGA
CGGTGCCATCGTGCTGCGCGGCTTTGCGCGCACGCAGGCGCCGCAATTGCTCGACGAGATCGGAGACATTGCTGCAAAAG
CGCCCTGGCGCCACATGATCACGCCGGGTGGCTTGCGCATGTCGGTGGCCATGACCAACTGCGGGCAGGCCGGCTGGGTC
TCCGACAGGACGGGCTACCGCTATGATCCATGCGATCCGCTCGATGGCGAGCCATGGCCGGCCATGCCCGCGGCATTCCT
CGACCTGGCCACGCGTGCCGCGGCCGAGGCAGGCTTTGCCTGCTTTGCGCCGGATGCCTGCCTGATCAACCGGTATGAGC
CGGGCACCCGGCTTTCCTTGCATCAGGATCGCGACGAGCGCGATCTTCGCGCCCCGATCGTATCGGTATCGCTGGGCCTG
CCGGCTGTGTTCCTGTTCGGCGGCTTGCGGCGCGCCGACCGGCCTGCGCGCATACGGCTCGCCCATGGCGACGTGGTGGT
ATGGGGCGGCCCTTCGCGCCTGGCCTTCCATGGCGTGGCGCCGCTGGCCGATGGTGACCACCCGCTGCTCGGGCAGAAAC
GGATCAACCTGACCTTCCGCAGGGCGCTGTAG

Upstream 100 bases:

>100_bases
TTGTGCGCGATACACCGCGCCACAGCGTTCCGGCGGCGCCGGTGCATCGGAGCGATAATTCCCGCAGGTTGCCCTGCCAT
CCTGCCTGTGCCACTGCCCC

Downstream 100 bases:

>100_bases
CCGCTGCGGCAAAGCGTCCGGTCAGGGTACCGGGGCAGCCACCGTAGTGAACGACCAGCTTGTGCTCAGCGGCGTGCCGG
CGCGCGCGCCGGCGAAGCTC

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTFDLFDNLDNSQDGYAPGTAPEPLADGAIVLRGFARTQAPQLLDEIGDIAAKAPWRHMITPGGLRMSVAMTNCGQAGWV
SDRTGYRYDPCDPLDGEPWPAMPAAFLDLATRAAAEAGFACFAPDACLINRYEPGTRLSLHQDRDERDLRAPIVSVSLGL
PAVFLFGGLRRADRPARIRLAHGDVVVWGGPSRLAFHGVAPLADGDHPLLGQKRINLTFRRAL

Sequences:

>Translated_223_residues
MTFDLFDNLDNSQDGYAPGTAPEPLADGAIVLRGFARTQAPQLLDEIGDIAAKAPWRHMITPGGLRMSVAMTNCGQAGWV
SDRTGYRYDPCDPLDGEPWPAMPAAFLDLATRAAAEAGFACFAPDACLINRYEPGTRLSLHQDRDERDLRAPIVSVSLGL
PAVFLFGGLRRADRPARIRLAHGDVVVWGGPSRLAFHGVAPLADGDHPLLGQKRINLTFRRAL
>Mature_222_residues
TFDLFDNLDNSQDGYAPGTAPEPLADGAIVLRGFARTQAPQLLDEIGDIAAKAPWRHMITPGGLRMSVAMTNCGQAGWVS
DRTGYRYDPCDPLDGEPWPAMPAAFLDLATRAAAEAGFACFAPDACLINRYEPGTRLSLHQDRDERDLRAPIVSVSLGLP
AVFLFGGLRRADRPARIRLAHGDVVVWGGPSRLAFHGVAPLADGDHPLLGQKRINLTFRRAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=122, Percent_Identity=33.6065573770492, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788541, Length=200, Percent_Identity=62.5, Blast_Score=259, Evalue=9e-71,
Organism=Drosophila melanogaster, GI45555401, Length=105, Percent_Identity=38.0952380952381, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24143; Mature: 24012

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDLFDNLDNSQDGYAPGTAPEPLADGAIVLRGFARTQAPQLLDEIGDIAAKAPWRHMI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
TPGGLRMSVAMTNCGQAGWVSDRTGYRYDPCDPLDGEPWPAMPAAFLDLATRAAAEAGFA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
CFAPDACLINRYEPGTRLSLHQDRDERDLRAPIVSVSLGLPAVFLFGGLRRADRPARIRL
EECCCHHEEECCCCCCEEEECCCCCCHHHHCCEEEEECCHHHHHHHHHHHCCCCCCEEEE
AHGDVVVWGGPSRLAFHGVAPLADGDHPLLGQKRINLTFRRAL
ECCCEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
TFDLFDNLDNSQDGYAPGTAPEPLADGAIVLRGFARTQAPQLLDEIGDIAAKAPWRHMI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
TPGGLRMSVAMTNCGQAGWVSDRTGYRYDPCDPLDGEPWPAMPAAFLDLATRAAAEAGFA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
CFAPDACLINRYEPGTRLSLHQDRDERDLRAPIVSVSLGLPAVFLFGGLRRADRPARIRL
EECCCHHEEECCCCCCEEEECCCCCCHHHHCCEEEEECCHHHHHHHHHHHCCCCCCEEEE
AHGDVVVWGGPSRLAFHGVAPLADGDHPLLGQKRINLTFRRAL
ECCCEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]