Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 73538785

GI number: 73538785

Start: 1688855

End: 1690096

Strand: Reverse

Name: dosC [H]

Synonym: Reut_B4960

Alternate gene names: 73538785

Gene position: 1690096-1688855 (Counterclockwise)

Preceding gene: 73538786

Following gene: 73538781

Centisome position: 62.0

GC content: 68.84

Gene sequence:

>1242_bases
GTGCACCTGGACCAGCAGACCATTGCGGTCGTCATGATGGTGTTCTTTTCCAGCACGCTGGTCATTGCGGCCGGGCTGGT
CTTTGCGCTGCGCGCGATCGAAGCGGGGCGGCTATGGGCGTTCGGCCACGTGGTTGGCTCCGGCGCCGGGCTGGCGCTGG
CGGTCAGCGCGGCCGGCGGGGCGCCGCACATCGGCATGGCCGGGGCCGCTGCCTTCATGGTGGGCTGGCTGCTGATCTAT
CGCGGCGTTCGCGTCTACTACGGCCTGCCGACGCACGCGGCGCCACTGGCCGTTGCAGGCCTGGTCGTGCTCGCGCTGAT
GGCGACCTCCGGCGGCCTGCCGCAGGGAACGCGCATGCTGCAGGCGGGGGTCTATGTCGTGCTGGCCATGATGTCGCTGG
CGACGATCGGTACGCTCGTAACCACCGGGCGCGGACGGCGCAGCATCGGCGCCCCGCTGGTGCTGGTGTCGAGCCTGCTG
CAGCTGTCCACCCAGCTCGTCGGGGCCAGCCATGCGCTTAGCCAGGTTGCCGCCGAGCGTCAAGACCTGACCCTGTTCTT
CGCTGGCCCGGCCGGTTCGGCCTGGGCGCTGGCACCGCTGATCGCCGTCCTGCTGGGCCTGTTCGGCTTCACGGTGATGG
CGATGGAGCAGATCATCGCCAACAACGAAAGCGGCGCGCGCGTGGACGCGCTGACCGGCCTGCTCAATCGCGGCGCGCTC
GACATGTCGGCACTTAGCCTGGTTGCGCGCTGGCAGCGCGACGGGCAGTCGCTGTCATGTCTGGTCATTGATATCGACCA
CTTCAAGCAGGTCAACGATACCTTCGGCCACCATGCCGGCGATGCCGTGCTGCGCGAAGTGGCCCAGGCGCTGGACAACT
CGCGCCGCGCCTCGGACGTGGCCGGACGCTACGGCGGCGAGGAATTCTGCATCCTCTGCCCGCATACCGACGAACACCAG
GCCACCGCGCTGGCCAACCGTATCCTTCGCAAGGTCCGCGCCATTCCGCTGCCGGGGAAGGAGTGCTTCGCGTCGGTGAG
CATCGGCGTCGCGCAGCTGCACGGTGCAACGGGTAGCAAGGAGCTGCTGTGGCGCACCTTGTTCGCGGATGCCGACCGCG
CCCTCTACCAGGCCAAGCAGCAGGGCCGCGATCGCTATGAGGTGGCGTCCGCCATGCTCGACGAGAAGCCGGCGCCGCTG
CCGGTCCTCGTGAACAGCCAGATCATGACGCCGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGAGCGGGATATCATTCGCGGTCGCCCCCCTTTACCCCGTGCCGTCATGTCGATTCGAAACCGATCGACGGCATCCTCAT
GGCCTTGTTAAGGGATTCTC

Downstream 100 bases:

>100_bases
ACGGCGTCAGTCTCCCTGCGCTGGCACATCGCGTTGCGGCGTGCCGTCGTAGGTCCAAAGGAACTGGCGCGGGATCGGGC
CCTTGTTGCGGCCGCCTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MHLDQQTIAVVMMVFFSSTLVIAAGLVFALRAIEAGRLWAFGHVVGSGAGLALAVSAAGGAPHIGMAGAAAFMVGWLLIY
RGVRVYYGLPTHAAPLAVAGLVVLALMATSGGLPQGTRMLQAGVYVVLAMMSLATIGTLVTTGRGRRSIGAPLVLVSSLL
QLSTQLVGASHALSQVAAERQDLTLFFAGPAGSAWALAPLIAVLLGLFGFTVMAMEQIIANNESGARVDALTGLLNRGAL
DMSALSLVARWQRDGQSLSCLVIDIDHFKQVNDTFGHHAGDAVLREVAQALDNSRRASDVAGRYGGEEFCILCPHTDEHQ
ATALANRILRKVRAIPLPGKECFASVSIGVAQLHGATGSKELLWRTLFADADRALYQAKQQGRDRYEVASAMLDEKPAPL
PVLVNSQIMTPAA

Sequences:

>Translated_413_residues
MHLDQQTIAVVMMVFFSSTLVIAAGLVFALRAIEAGRLWAFGHVVGSGAGLALAVSAAGGAPHIGMAGAAAFMVGWLLIY
RGVRVYYGLPTHAAPLAVAGLVVLALMATSGGLPQGTRMLQAGVYVVLAMMSLATIGTLVTTGRGRRSIGAPLVLVSSLL
QLSTQLVGASHALSQVAAERQDLTLFFAGPAGSAWALAPLIAVLLGLFGFTVMAMEQIIANNESGARVDALTGLLNRGAL
DMSALSLVARWQRDGQSLSCLVIDIDHFKQVNDTFGHHAGDAVLREVAQALDNSRRASDVAGRYGGEEFCILCPHTDEHQ
ATALANRILRKVRAIPLPGKECFASVSIGVAQLHGATGSKELLWRTLFADADRALYQAKQQGRDRYEVASAMLDEKPAPL
PVLVNSQIMTPAA
>Mature_413_residues
MHLDQQTIAVVMMVFFSSTLVIAAGLVFALRAIEAGRLWAFGHVVGSGAGLALAVSAAGGAPHIGMAGAAAFMVGWLLIY
RGVRVYYGLPTHAAPLAVAGLVVLALMATSGGLPQGTRMLQAGVYVVLAMMSLATIGTLVTTGRGRRSIGAPLVLVSSLL
QLSTQLVGASHALSQVAAERQDLTLFFAGPAGSAWALAPLIAVLLGLFGFTVMAMEQIIANNESGARVDALTGLLNRGAL
DMSALSLVARWQRDGQSLSCLVIDIDHFKQVNDTFGHHAGDAVLREVAQALDNSRRASDVAGRYGGEEFCILCPHTDEHQ
ATALANRILRKVRAIPLPGKECFASVSIGVAQLHGATGSKELLWRTLFADADRALYQAKQQGRDRYEVASAMLDEKPAPL
PVLVNSQIMTPAA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=201, Percent_Identity=36.81592039801, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786584, Length=220, Percent_Identity=30.9090909090909, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=41.9753086419753, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=39.4117647058824, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787262, Length=212, Percent_Identity=32.5471698113208, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081974, Length=198, Percent_Identity=32.3232323232323, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=28.7292817679558, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=197, Percent_Identity=32.48730964467, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=33.9506172839506, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=28.4810126582279, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=28.6585365853659, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43355; Mature: 43355

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLDQQTIAVVMMVFFSSTLVIAAGLVFALRAIEAGRLWAFGHVVGSGAGLALAVSAAGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCEEEEEECCCC
APHIGMAGAAAFMVGWLLIYRGVRVYYGLPTHAAPLAVAGLVVLALMATSGGLPQGTRML
CCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QAGVYVVLAMMSLATIGTLVTTGRGRRSIGAPLVLVSSLLQLSTQLVGASHALSQVAAER
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDLTLFFAGPAGSAWALAPLIAVLLGLFGFTVMAMEQIIANNESGARVDALTGLLNRGAL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
DMSALSLVARWQRDGQSLSCLVIDIDHFKQVNDTFGHHAGDAVLREVAQALDNSRRASDV
HHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
AGRYGGEEFCILCPHTDEHQATALANRILRKVRAIPLPGKECFASVSIGVAQLHGATGSK
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCH
ELLWRTLFADADRALYQAKQQGRDRYEVASAMLDEKPAPLPVLVNSQIMTPAA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MHLDQQTIAVVMMVFFSSTLVIAAGLVFALRAIEAGRLWAFGHVVGSGAGLALAVSAAGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCEEEEEECCCC
APHIGMAGAAAFMVGWLLIYRGVRVYYGLPTHAAPLAVAGLVVLALMATSGGLPQGTRML
CCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QAGVYVVLAMMSLATIGTLVTTGRGRRSIGAPLVLVSSLLQLSTQLVGASHALSQVAAER
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDLTLFFAGPAGSAWALAPLIAVLLGLFGFTVMAMEQIIANNESGARVDALTGLLNRGAL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
DMSALSLVARWQRDGQSLSCLVIDIDHFKQVNDTFGHHAGDAVLREVAQALDNSRRASDV
HHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
AGRYGGEEFCILCPHTDEHQATALANRILRKVRAIPLPGKECFASVSIGVAQLHGATGSK
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCH
ELLWRTLFADADRALYQAKQQGRDRYEVASAMLDEKPAPLPVLVNSQIMTPAA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]