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The map label for this gene is dfrA [H]

Identifier: 73538722

GI number: 73538722

Start: 1620855

End: 1621856

Strand: Reverse

Name: dfrA [H]

Synonym: Reut_B4897

Alternate gene names: 73538722

Gene position: 1621856-1620855 (Counterclockwise)

Preceding gene: 73538729

Following gene: 73538714

Centisome position: 59.49

GC content: 67.27

Gene sequence:

>1002_bases
ATGACCGTAAACGATTACGCGCTGGTGACAGGTGCATCGGGTTTTCTCGGATCCGCGGTAGTGCGGCAGGCACTGGCGCG
CGGCTGGCGCGTGCGCGCGCTGGTGCGGGCGACGAGCCCGCGCGCCAATCTGGATGGTCTGCCCGTCGAGGTGTTCGAGG
GAGATATGCGCGACCCCGCCGCGCTGACGTCCGCCATGCGCGGGGTGCGCTACCTGTTCCATGTGGCGGCCGATTATCGG
CTATGGGCCCCTGATCCCGAGGAAATCGTCCGCACCAATGTCGACGGGACCATGGCGGTCATGGAAGCCGCGCAGGAGGC
AGGGGTAGAGCGCGTTGTCTATACCAGCAGCGTGGCGACGTTGCGGGTGGCGGGTGCCTCGGCGCCGGTCGACGAGACGG
CCGCCATGACACCGCACGAGGCCATCGGCGCGTACAAGCGCAGCAAGGTGCTGGCCGAGCGTGTGGTCGAGCGCATGGTC
GCCGAGCGCGGGTTGCCGGCCGTGATCGTCAATCCGTCCACGCCGATCGGGCCGCGGGACGTCCGGCCTACGCCGACCGG
ACGCATCATCGTCGAGGCCGCGACCGGCAAGATTCCGGCCTTTGTCGAGACCGGGCTCAACCTTGTCCATGTCGATGACG
TCGCCGCCGGGCATTTCCAGGCGCTGGAGCGAGGCCGCATCGGCGAACGCTACATCCTGGGCGGTGACGACGTGATGCTG
CAGCAGATGCTGCGCGATATCGCGGATTTGTGCGGACGCCGTCCGCCAACCGTGCAACTGCCGCGCTGGCCGCTGTATCC
GCTGGCGCATGCCGCGGAGGCGGTGGCGCGCATCACGCGCAAAGAGCCCTTTGTCACCGTGGATGGGCTCCATATGTCGA
AGTACCGGATGTTTTTCCGCTCGGACAAGGCCCGGCAGGAGCTTGGCTATCAGCCGCGTCCTTATATTGATGGCCTGCGC
GACGCGCTGGACTGGTTCCGCGAGGCCGGTTACCTGCGCTGA

Upstream 100 bases:

>100_bases
CATTTCCGACACATGGTGGTTACTATATACCCGCCGCGCCGGAAATGTCTGGCAGCCCTCTCAGTCGGGCGGCATTCCCA
TCTCACGATGAAACGGGGTT

Downstream 100 bases:

>100_bases
GGGCTCAGGACCGTGCGGGCGCCTGGCTGCGTCCTTTCCACTGGCCGCCGCGGCGCAGCCAGTAGCGGCGTGCGGAATCC
AGCGTGGCGCCAAGATAGAA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTVNDYALVTGASGFLGSAVVRQALARGWRVRALVRATSPRANLDGLPVEVFEGDMRDPAALTSAMRGVRYLFHVAADYR
LWAPDPEEIVRTNVDGTMAVMEAAQEAGVERVVYTSSVATLRVAGASAPVDETAAMTPHEAIGAYKRSKVLAERVVERMV
AERGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVETGLNLVHVDDVAAGHFQALERGRIGERYILGGDDVML
QQMLRDIADLCGRRPPTVQLPRWPLYPLAHAAEAVARITRKEPFVTVDGLHMSKYRMFFRSDKARQELGYQPRPYIDGLR
DALDWFREAGYLR

Sequences:

>Translated_333_residues
MTVNDYALVTGASGFLGSAVVRQALARGWRVRALVRATSPRANLDGLPVEVFEGDMRDPAALTSAMRGVRYLFHVAADYR
LWAPDPEEIVRTNVDGTMAVMEAAQEAGVERVVYTSSVATLRVAGASAPVDETAAMTPHEAIGAYKRSKVLAERVVERMV
AERGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVETGLNLVHVDDVAAGHFQALERGRIGERYILGGDDVML
QQMLRDIADLCGRRPPTVQLPRWPLYPLAHAAEAVARITRKEPFVTVDGLHMSKYRMFFRSDKARQELGYQPRPYIDGLR
DALDWFREAGYLR
>Mature_332_residues
TVNDYALVTGASGFLGSAVVRQALARGWRVRALVRATSPRANLDGLPVEVFEGDMRDPAALTSAMRGVRYLFHVAADYRL
WAPDPEEIVRTNVDGTMAVMEAAQEAGVERVVYTSSVATLRVAGASAPVDETAAMTPHEAIGAYKRSKVLAERVVERMVA
ERGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVETGLNLVHVDDVAAGHFQALERGRIGERYILGGDDVMLQ
QMLRDIADLCGRRPPTVQLPRWPLYPLAHAAEAVARITRKEPFVTVDGLHMSKYRMFFRSDKARQELGYQPRPYIDGLRD
ALDWFREAGYLR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=331, Percent_Identity=28.0966767371601, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI8393516, Length=331, Percent_Identity=28.0966767371601, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI116268111, Length=363, Percent_Identity=25.6198347107438, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI310132178, Length=338, Percent_Identity=26.3313609467456, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310113012, Length=338, Percent_Identity=26.3313609467456, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI239745448, Length=338, Percent_Identity=26.3313609467456, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71987463, Length=243, Percent_Identity=30.8641975308642, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321437, Length=351, Percent_Identity=24.2165242165242, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36631; Mature: 36499

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNDYALVTGASGFLGSAVVRQALARGWRVRALVRATSPRANLDGLPVEVFEGDMRDPA
CCCCCEEEEECCCHHHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCCCHHHHCCCCCCHH
ALTSAMRGVRYLFHVAADYRLWAPDPEEIVRTNVDGTMAVMEAAQEAGVERVVYTSSVAT
HHHHHHHHHHHHHHHHHCCEECCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCEEE
LRVAGASAPVDETAAMTPHEAIGAYKRSKVLAERVVERMVAERGLPAVIVNPSTPIGPRD
EEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
VRPTPTGRIIVEAATGKIPAFVETGLNLVHVDDVAAGHFQALERGRIGERYILGGDDVML
CCCCCCCCEEEEECCCCCCHHHHCCCCEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHH
QQMLRDIADLCGRRPPTVQLPRWPLYPLAHAAEAVARITRKEPFVTVDGLHMSKYRMFFR
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
SDKARQELGYQPRPYIDGLRDALDWFREAGYLR
CCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVNDYALVTGASGFLGSAVVRQALARGWRVRALVRATSPRANLDGLPVEVFEGDMRDPA
CCCCEEEEECCCHHHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCCCHHHHCCCCCCHH
ALTSAMRGVRYLFHVAADYRLWAPDPEEIVRTNVDGTMAVMEAAQEAGVERVVYTSSVAT
HHHHHHHHHHHHHHHHHCCEECCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCEEE
LRVAGASAPVDETAAMTPHEAIGAYKRSKVLAERVVERMVAERGLPAVIVNPSTPIGPRD
EEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
VRPTPTGRIIVEAATGKIPAFVETGLNLVHVDDVAAGHFQALERGRIGERYILGGDDVML
CCCCCCCCEEEEECCCCCCHHHHCCCCEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHH
QQMLRDIADLCGRRPPTVQLPRWPLYPLAHAAEAVARITRKEPFVTVDGLHMSKYRMFFR
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
SDKARQELGYQPRPYIDGLRDALDWFREAGYLR
CCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]