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The map label for this gene is sdaA [H]

Identifier: 73538656

GI number: 73538656

Start: 1535794

End: 1537182

Strand: Reverse

Name: sdaA [H]

Synonym: Reut_B4831

Alternate gene names: 73538656

Gene position: 1537182-1535794 (Counterclockwise)

Preceding gene: 73538660

Following gene: 73538655

Centisome position: 56.39

GC content: 64.07

Gene sequence:

>1389_bases
ATGAATGTCAGCGTTTTCGACCTGTTCAAAATCGGCATCGGTCCATCGAGTTCGCATACGGTCGGCCCGATGATTGCCGC
ATGCCGATTCGTCTCGCACCTTGAGGATGCGAACCTGCTCGGCTTCGTGCGCCGCGTGCGGGCCGAATTGTACGGTTCGC
TCGGTGCGACCGGAAAGGGCCATGGCACGGACAAGGCGGTGCTGCTCGGCCTTGAGGGAAGCTTGCCCGACCGCATCGAT
CCGGACGTCATTGAGCCGCGGCTCGCGCAGATCCGCAAGGACAAGCGCCTGTTGCTGCTTGGCAAGCATGAGATTGCGTT
CGACGAAAAGGAGCACATCGGCTTCTTTCGCCGTCTGATGAGTGGCACGGGCAATGTCGTGCATCCGAACGGCATGCGCT
TCCAGGCGTTCGACGAGCAGGGCCAGTTGCTCGTCGAGAAAGAGTACTACTCGATCGGTGGCGGCTTCGTGGTCAATCGC
GACGGCGATCGCGTCAACGGCGTGCGCGCGGCTGCCGAGGTGCCATATCCGTTCCGCACCGGTGCGGACCTGATGCAGGC
GTGCAGCGACAGCGGGCTGTCGATCGCGGCGCTGATCTTTCGCAATGAATGCGCGCTGCGGCCGGCCGAAGACGTACGCG
CGGGGCTGCTGGCGATCTGGCAAACGATGGCCGCCTGCGTCGAGCGTGGCTGCAAGGTCGAGGGCGAGCTGCCCGGCCCG
ATGCGCGTGAAGCGCCGCGCGGCCGAACTCAATCACCGCTTGCGCACACGTTCCGAGGAGTCGCTGCGTGATCCGCTGTC
GATGCTCGACTGGGTCAACCTGTATGCGATGGCCGTCAACGAGGAGAACGCAGCGGGCGGCCGCGTCGTCACCGCACCGA
CAAACGGCGCGGCAGGCGTGATCCCCGCGGTGCTCCACTACTACGTGAAGTTCGTGCCCGGTTCGAATGACGAGGGCATC
GTCGAGTTCCTGCTGACGGCAGCCGCCATCGGGATGATCTACAAGGAGACGGCCTCCATCTCGGGTGCCGAGGTTGGTTG
CCAGGGGGAGGTGGGCGTGGCGTGCTCGATGGCCGCGGCCGCGCTGGCCGCCGTGATGGGCGGTACGCCTGATCAGGTCG
AGAATGCCGCGGAAATCGGCATGGAGCACAACCTCGGCATGACCTGCGATCCGGTCGGCGGGCTGGTGCAGATTCCGTGT
ATCGAGCGCAATGCGATGGGCGCAATCAAGGCGCTCAATGCATCTCGCATGGCGCTGAAGGGCGATGGCCAGCACTACGT
GTCGCTCGATTCTGTGATCAAGACCATGCGCGAAACCGGCGCCGACATGAAGACGAAGTACAAGGAGACGTCGCGCGGCG
GCCTGGCCGTGAACGTCATTGAATGCTAA

Upstream 100 bases:

>100_bases
GTCCCACGTCGATGCCGCTTTTGTTCTATCGGCAAATTCCACGCTTTGATGTACTGGCGTCCAACGACACCTACCAGCCA
AGAGCAAAGAGGCAAGCCAG

Downstream 100 bases:

>100_bases
CGAGGGTCCATGAAATGAGCCGCTATTCGATATTCAGTCTGTTCCGCAACGGCCTGTCCTATCACGAAAACTGGGAAAAG
CAATGGAAGAGCCCGGAGCC

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNVSVFDLFKIGIGPSSSHTVGPMIAACRFVSHLEDANLLGFVRRVRAELYGSLGATGKGHGTDKAVLLGLEGSLPDRID
PDVIEPRLAQIRKDKRLLLLGKHEIAFDEKEHIGFFRRLMSGTGNVVHPNGMRFQAFDEQGQLLVEKEYYSIGGGFVVNR
DGDRVNGVRAAAEVPYPFRTGADLMQACSDSGLSIAALIFRNECALRPAEDVRAGLLAIWQTMAACVERGCKVEGELPGP
MRVKRRAAELNHRLRTRSEESLRDPLSMLDWVNLYAMAVNEENAAGGRVVTAPTNGAAGVIPAVLHYYVKFVPGSNDEGI
VEFLLTAAAIGMIYKETASISGAEVGCQGEVGVACSMAAAALAAVMGGTPDQVENAAEIGMEHNLGMTCDPVGGLVQIPC
IERNAMGAIKALNASRMALKGDGQHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNVIEC

Sequences:

>Translated_462_residues
MNVSVFDLFKIGIGPSSSHTVGPMIAACRFVSHLEDANLLGFVRRVRAELYGSLGATGKGHGTDKAVLLGLEGSLPDRID
PDVIEPRLAQIRKDKRLLLLGKHEIAFDEKEHIGFFRRLMSGTGNVVHPNGMRFQAFDEQGQLLVEKEYYSIGGGFVVNR
DGDRVNGVRAAAEVPYPFRTGADLMQACSDSGLSIAALIFRNECALRPAEDVRAGLLAIWQTMAACVERGCKVEGELPGP
MRVKRRAAELNHRLRTRSEESLRDPLSMLDWVNLYAMAVNEENAAGGRVVTAPTNGAAGVIPAVLHYYVKFVPGSNDEGI
VEFLLTAAAIGMIYKETASISGAEVGCQGEVGVACSMAAAALAAVMGGTPDQVENAAEIGMEHNLGMTCDPVGGLVQIPC
IERNAMGAIKALNASRMALKGDGQHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNVIEC
>Mature_462_residues
MNVSVFDLFKIGIGPSSSHTVGPMIAACRFVSHLEDANLLGFVRRVRAELYGSLGATGKGHGTDKAVLLGLEGSLPDRID
PDVIEPRLAQIRKDKRLLLLGKHEIAFDEKEHIGFFRRLMSGTGNVVHPNGMRFQAFDEQGQLLVEKEYYSIGGGFVVNR
DGDRVNGVRAAAEVPYPFRTGADLMQACSDSGLSIAALIFRNECALRPAEDVRAGLLAIWQTMAACVERGCKVEGELPGP
MRVKRRAAELNHRLRTRSEESLRDPLSMLDWVNLYAMAVNEENAAGGRVVTAPTNGAAGVIPAVLHYYVKFVPGSNDEGI
VEFLLTAAAIGMIYKETASISGAEVGCQGEVGVACSMAAAALAAVMGGTPDQVENAAEIGMEHNLGMTCDPVGGLVQIPC
IERNAMGAIKALNASRMALKGDGQHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNVIEC

Specific function: Deaminates Also Threonine, Particularly When It Is Present In High Concentration. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1789161, Length=463, Percent_Identity=52.6997840172786, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1788116, Length=461, Percent_Identity=52.2776572668113, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI48994925, Length=459, Percent_Identity=52.0697167755991, Blast_Score=458, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 49479; Mature: 49479

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSVFDLFKIGIGPSSSHTVGPMIAACRFVSHLEDANLLGFVRRVRAELYGSLGATGKG
CCCEEEEHEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
HGTDKAVLLGLEGSLPDRIDPDVIEPRLAQIRKDKRLLLLGKHEIAFDEKEHIGFFRRLM
CCCCCEEEEEECCCCCCCCCCHHCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
SGTGNVVHPNGMRFQAFDEQGQLLVEKEYYSIGGGFVVNRDGDRVNGVRAAAEVPYPFRT
HCCCCEECCCCCEEEEECCCCCEEEEEHHEECCCCEEEECCCCCCCCCHHHHCCCCCCCC
GADLMQACSDSGLSIAALIFRNECALRPAEDVRAGLLAIWQTMAACVERGCKVEGELPGP
CHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCH
MRVKRRAAELNHRLRTRSEESLRDPLSMLDWVNLYAMAVNEENAAGGRVVTAPTNGAAGV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHH
IPAVLHYYVKFVPGSNDEGIVEFLLTAAAIGMIYKETASISGAEVGCQGEVGVACSMAAA
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
ALAAVMGGTPDQVENAAEIGMEHNLGMTCDPVGGLVQIPCIERNAMGAIKALNASRMALK
HHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEECCCHHHHHHHCCCCCEEEE
GDGQHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNVIEC
CCCCEEEEHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MNVSVFDLFKIGIGPSSSHTVGPMIAACRFVSHLEDANLLGFVRRVRAELYGSLGATGKG
CCCEEEEHEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
HGTDKAVLLGLEGSLPDRIDPDVIEPRLAQIRKDKRLLLLGKHEIAFDEKEHIGFFRRLM
CCCCCEEEEEECCCCCCCCCCHHCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
SGTGNVVHPNGMRFQAFDEQGQLLVEKEYYSIGGGFVVNRDGDRVNGVRAAAEVPYPFRT
HCCCCEECCCCCEEEEECCCCCEEEEEHHEECCCCEEEECCCCCCCCCHHHHCCCCCCCC
GADLMQACSDSGLSIAALIFRNECALRPAEDVRAGLLAIWQTMAACVERGCKVEGELPGP
CHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCH
MRVKRRAAELNHRLRTRSEESLRDPLSMLDWVNLYAMAVNEENAAGGRVVTAPTNGAAGV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHH
IPAVLHYYVKFVPGSNDEGIVEFLLTAAAIGMIYKETASISGAEVGCQGEVGVACSMAAA
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
ALAAVMGGTPDQVENAAEIGMEHNLGMTCDPVGGLVQIPCIERNAMGAIKALNASRMALK
HHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEECCCHHHHHHHCCCCCEEEE
GDGQHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNVIEC
CCCCEEEEHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]