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The map label for this gene is ada [H]

Identifier: 73538559

GI number: 73538559

Start: 1433467

End: 1434189

Strand: Direct

Name: ada [H]

Synonym: Reut_B4734

Alternate gene names: 73538559

Gene position: 1433467-1434189 (Clockwise)

Preceding gene: 73538558

Following gene: 73538560

Centisome position: 52.58

GC content: 69.57

Gene sequence:

>723_bases
TTGCCGTCCCAGTGCTTCCGCGCGCCTGCCGAAGCGCGAGAACGTCGAGTTCTTCGATACGCCCGCAGCGGCCGAAGCCG
CCGGATACCGAGCCAGCCGCCGTGCTCGCGCCGACCGGACCAGCGCGGCCGCCGGGCGCGCTGCGCTCGTGGCGGAGGTC
TGCCGACTGCCTCCGACCAGTTGCTCGGCATGCGCGCGCGCGATTACCGCGCCGGCGGCACCGGCGCGGTGATTCACTTC
GCGGTCGGCCAGTGTTCGCTAGGCGCCATCCTGGTCGCGCAAAGCCAGCGCGGCATCTGCGCGATCCTGCTCAACGATGA
CCCGGATGCGCTCGTGCGCGAGCTGCAAGACCAGTTTCCCAATGCCCAGCTGATCGGTGGCGATGCCGGCTTCGAAAATC
TCGTGGCCCAGGTGGTCGGCTTCGTTGAGGCGCCATCCATCGGTCTCAACCTGCCGCTCGACGTCCGTGGCACCGCGTTC
CAGGAACGCGTCTGGCAGGCCTTGCGCGAGATCCCGCCCGGCACGACCGTGAGCTATGCGGAGATCGCCGAACGTATCGG
CAGGCCCACGGCCACGCGCGCCGTCGCTCAGGCTTGCGGCGCCAACCACATTGCCATCGCGATTCCGTGCCACCGCGTCG
TCCGCCGCGATGGCGACATCGCTGGCTATCGCTGGGGCGTGGATCGCAAGCGCGAACTGCTGCGGCGCGAAGCCGAAGCC
TGA

Upstream 100 bases:

>100_bases
CCATGTACGCGAGCGAAGCATCGCGCTGGCAGGCAGTACAGGCGCGCGACGCCGCCGCTGACGGATACTTTGTCTACGCC
GTGCGCACGACAGGCGTCTA

Downstream 100 bases:

>100_bases
CCGGGACGATGCGATGCCCTCCTCCGCTATGACATTCGACCGGCTGGACTGGCAGGAGATCGCTGCACAACTCGATCAGG
ACGGGTATGCCGGGCTGCCC

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MPSQCFRAPAEARERRVLRYARSGRSRRIPSQPPCSRRPDQRGRRARCARGGGLPTASDQLLGMRARDYRAGGTGAVIHF
AVGQCSLGAILVAQSQRGICAILLNDDPDALVRELQDQFPNAQLIGGDAGFENLVAQVVGFVEAPSIGLNLPLDVRGTAF
QERVWQALREIPPGTTVSYAEIAERIGRPTATRAVAQACGANHIAIAIPCHRVVRRDGDIAGYRWGVDRKRELLRREAEA

Sequences:

>Translated_240_residues
MPSQCFRAPAEARERRVLRYARSGRSRRIPSQPPCSRRPDQRGRRARCARGGGLPTASDQLLGMRARDYRAGGTGAVIHF
AVGQCSLGAILVAQSQRGICAILLNDDPDALVRELQDQFPNAQLIGGDAGFENLVAQVVGFVEAPSIGLNLPLDVRGTAF
QERVWQALREIPPGTTVSYAEIAERIGRPTATRAVAQACGANHIAIAIPCHRVVRRDGDIAGYRWGVDRKRELLRREAEA
>Mature_239_residues
PSQCFRAPAEARERRVLRYARSGRSRRIPSQPPCSRRPDQRGRRARCARGGGLPTASDQLLGMRARDYRAGGTGAVIHFA
VGQCSLGAILVAQSQRGICAILLNDDPDALVRELQDQFPNAQLIGGDAGFENLVAQVVGFVEAPSIGLNLPLDVRGTAFQ
ERVWQALREIPPGTTVSYAEIAERIGRPTATRAVAQACGANHIAIAIPCHRVVRRDGDIAGYRWGVDRKRELLRREAEA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=195, Percent_Identity=53.3333333333333, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI1787596, Length=98, Percent_Identity=48.9795918367347, Blast_Score=94, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=126, Percent_Identity=39.6825396825397, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=161, Percent_Identity=37.888198757764, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=45.7831325301205, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137554, Length=140, Percent_Identity=38.5714285714286, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 26169; Mature: 26038

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSQCFRAPAEARERRVLRYARSGRSRRIPSQPPCSRRPDQRGRRARCARGGGLPTASDQ
CCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHH
LLGMRARDYRAGGTGAVIHFAVGQCSLGAILVAQSQRGICAILLNDDPDALVRELQDQFP
HHCCHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCC
NAQLIGGDAGFENLVAQVVGFVEAPSIGLNLPLDVRGTAFQERVWQALREIPPGTTVSYA
CCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
EIAERIGRPTATRAVAQACGANHIAIAIPCHRVVRRDGDIAGYRWGVDRKRELLRREAEA
HHHHHHCCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PSQCFRAPAEARERRVLRYARSGRSRRIPSQPPCSRRPDQRGRRARCARGGGLPTASDQ
CCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHH
LLGMRARDYRAGGTGAVIHFAVGQCSLGAILVAQSQRGICAILLNDDPDALVRELQDQFP
HHCCHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCC
NAQLIGGDAGFENLVAQVVGFVEAPSIGLNLPLDVRGTAFQERVWQALREIPPGTTVSYA
CCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
EIAERIGRPTATRAVAQACGANHIAIAIPCHRVVRRDGDIAGYRWGVDRKRELLRREAEA
HHHHHHCCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]