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The map label for this gene is yafC [C]

Identifier: 73538510

GI number: 73538510

Start: 1381354

End: 1382310

Strand: Reverse

Name: yafC [C]

Synonym: Reut_B4684

Alternate gene names: 73538510

Gene position: 1382310-1381354 (Counterclockwise)

Preceding gene: 73538514

Following gene: 73538508

Centisome position: 50.71

GC content: 65.83

Gene sequence:

>957_bases
ATGTCATTGCGAACCGAGGATATCGAGGCCTATCTTCACGTGGTGGAGCTTGGCGGCATCAGTGCGGCGGCCCGGCGGAT
GGGTCTGTCGAAATCCGTGATCAGCAAGCGCATCAGCGACCTGGAGCGCGAGCTCGGCGTGTCGCTGCTGCAGCGGTCCA
CGCGCAGTACCCGGCCATCGGAGAACGGCTGCTACTTCTACGAACAGGCCCGTGCCGCTCTGAGACAGCTCGACCATGCG
GCACAAACCGTATCCGACGCTGCCGGTGAACTACGCGGCGAATTGCGCGTGCTGGCGCCGATGACGTTCGGCACGCGCTG
GCTGTCGCCGCTGATCGCGGCGTTCGCGCGTGCCCATCCGCGCATGAACCTGATCCTGGAGCTCGACGACAAGCTTGTCG
ACGTGGGCTACGAACGCTATGACGTCGCTATCCGCATCACGCGCCTTGGCGACAGTTCGCTGATTGCGCGCCGGCTCACG
GTGAGCCGGCGGGTGCTGTGCTGCAGTCCCGAATATGCGGCACGCGCAGGCCTGCCACAGGGAATCGAGGACATCGCGCA
GCATGCCTGCCTTGCCTACAGCAATTCGCCGCCGGCACACGTGTGGGCGTTTGCCGCCGGTGACGAACATGGCAGTGTCC
GGAGCATCGCACCGCGCGGCGTGTTTACTGCCAACAACGGCGAAGTGCTGCGCGATGCGGCCCTGGCCGGACAGGGCCTG
GCCGTGCTGCCCCGCTTCATTGTCTGGGACGACTTGCATGCCGGTCGCCTGGTTGAAGTCCTTCCGCATCTGCCGCCGGT
AGACGACGGCATCTTTGCCGTCTACCCGCGCAGCGGCTTTGGCTCGGTCAAGCTGCGCACGCTGGTACAGTACCTCCAGG
CATCGCTCGCGCGGCCGCCATGGGAAGAGCCGCAAACGGCGGGTTCGCTCCGCCTGCTGCAGGGCGGAGCCAACTGA

Upstream 100 bases:

>100_bases
TAGAGCCACCTTCGTCCTAATACTATGCTTTGTCCATCGAATGAAGCAATAGATGCCCGCCATCCAAAGCTTGCTGGGCG
GGCCGGAACGGGGAACAGTC

Downstream 100 bases:

>100_bases
TTCGCTCGGCACGACGCTGCTGCGTGGTTCAGGCAAGCAGCGGATTGCGCTTCTCCGGGTCGATCCCGTACTTGTAGATG
GCCTGCCTCAGCACGCCGCG

Product: regulatory protein LysR

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSLRTEDIEAYLHVVELGGISAAARRMGLSKSVISKRISDLERELGVSLLQRSTRSTRPSENGCYFYEQARAALRQLDHA
AQTVSDAAGELRGELRVLAPMTFGTRWLSPLIAAFARAHPRMNLILELDDKLVDVGYERYDVAIRITRLGDSSLIARRLT
VSRRVLCCSPEYAARAGLPQGIEDIAQHACLAYSNSPPAHVWAFAAGDEHGSVRSIAPRGVFTANNGEVLRDAALAGQGL
AVLPRFIVWDDLHAGRLVEVLPHLPPVDDGIFAVYPRSGFGSVKLRTLVQYLQASLARPPWEEPQTAGSLRLLQGGAN

Sequences:

>Translated_318_residues
MSLRTEDIEAYLHVVELGGISAAARRMGLSKSVISKRISDLERELGVSLLQRSTRSTRPSENGCYFYEQARAALRQLDHA
AQTVSDAAGELRGELRVLAPMTFGTRWLSPLIAAFARAHPRMNLILELDDKLVDVGYERYDVAIRITRLGDSSLIARRLT
VSRRVLCCSPEYAARAGLPQGIEDIAQHACLAYSNSPPAHVWAFAAGDEHGSVRSIAPRGVFTANNGEVLRDAALAGQGL
AVLPRFIVWDDLHAGRLVEVLPHLPPVDDGIFAVYPRSGFGSVKLRTLVQYLQASLARPPWEEPQTAGSLRLLQGGAN
>Mature_317_residues
SLRTEDIEAYLHVVELGGISAAARRMGLSKSVISKRISDLERELGVSLLQRSTRSTRPSENGCYFYEQARAALRQLDHAA
QTVSDAAGELRGELRVLAPMTFGTRWLSPLIAAFARAHPRMNLILELDDKLVDVGYERYDVAIRITRLGDSSLIARRLTV
SRRVLCCSPEYAARAGLPQGIEDIAQHACLAYSNSPPAHVWAFAAGDEHGSVRSIAPRGVFTANNGEVLRDAALAGQGLA
VLPRFIVWDDLHAGRLVEVLPHLPPVDDGIFAVYPRSGFGSVKLRTLVQYLQASLARPPWEEPQTAGSLRLLQGGAN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=30.8970099667774, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789440, Length=299, Percent_Identity=31.1036789297659, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=31.9727891156463, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=31.8339100346021, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=29.4117647058824, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787128, Length=299, Percent_Identity=28.0936454849498, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787589, Length=273, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=26.5625, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789173, Length=240, Percent_Identity=25.8333333333333, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=149, Percent_Identity=28.1879194630872, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34727; Mature: 34596

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRTEDIEAYLHVVELGGISAAARRMGLSKSVISKRISDLERELGVSLLQRSTRSTRPS
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENGCYFYEQARAALRQLDHAAQTVSDAAGELRGELRVLAPMTFGTRWLSPLIAAFARAHP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
RMNLILELDDKLVDVGYERYDVAIRITRLGDSSLIARRLTVSRRVLCCSPEYAARAGLPQ
CEEEEEEECHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCEEEEECCCHHHHCCCCC
GIEDIAQHACLAYSNSPPAHVWAFAAGDEHGSVRSIAPRGVFTANNGEVLRDAALAGQGL
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEECCCCEEECCCCCHHHHHHHCCCCH
AVLPRFIVWDDLHAGRLVEVLPHLPPVDDGIFAVYPRSGFGSVKLRTLVQYLQASLARPP
HHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
WEEPQTAGSLRLLQGGAN
CCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
SLRTEDIEAYLHVVELGGISAAARRMGLSKSVISKRISDLERELGVSLLQRSTRSTRPS
CCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENGCYFYEQARAALRQLDHAAQTVSDAAGELRGELRVLAPMTFGTRWLSPLIAAFARAHP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
RMNLILELDDKLVDVGYERYDVAIRITRLGDSSLIARRLTVSRRVLCCSPEYAARAGLPQ
CEEEEEEECHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCEEEEECCCHHHHCCCCC
GIEDIAQHACLAYSNSPPAHVWAFAAGDEHGSVRSIAPRGVFTANNGEVLRDAALAGQGL
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEECCCCEEECCCCCHHHHHHHCCCCH
AVLPRFIVWDDLHAGRLVEVLPHLPPVDDGIFAVYPRSGFGSVKLRTLVQYLQASLARPP
HHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
WEEPQTAGSLRLLQGGAN
CCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]